AB157110

Anti-RPL23A antibody

Name: Anti-RPL23A antibody (ab157110) | Abcam
Brand: Abcam Limited
SKU: AB157110
Price: 545 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal RPL23A antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human 60S ribosomal protein L23a aa 100 to C-terminus.

View Alternative Names

Large ribosomal subunit protein uL23, 60S ribosomal protein L23a, RPL23A

2 Images

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Immunoprecipitation - Anti-RPL23A antibody (AB157110)

Detection of RPL23A by Western Blot of Immunprecipitate.

Immunoprecipitation of HeLa whole cell lysate at 6 μg/mg lysate (1 mg/IP; 20% of IP loaded/lane), using the following :

Lane 1 : IP with an alternative Rabbit anti-EXOSC1 antibody.
Lane 2 : IP with ab157110.
Lane 3 : Control IgG.

The subsequent Western blot of immunoprecipitates was done using ab157110 at 0.4 μg/ml.
Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-RPL23A antibody (ab157110)

Predicted band size: 18 kDa

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Western blot - Anti-RPL23A antibody (AB157110)

All lanes:

Western blot - Anti-RPL23A antibody (ab157110) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 18 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human 60S ribosomal protein L23a aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P62750

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Plants": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Soybean": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Turkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPL23A also known as Ribosomal Protein L23a is a component of the 60S subunit in the ribosome which is responsible for protein synthesis. It has a molecular mass of approximately 15 kDa. RPL23A is expressed in various tissues but shows significant expression levels in the liver and pancreas. The protein plays a mechanical role by ensuring structural stability and proper functioning of the ribosome during translation.

Biological function summary

RPL23A participates in the assembly and function of the ribosomal complex critical for translation. Within this complex RPL23A contributes to the coordination of protein synthesis by helping to align mRNA and tRNA correctly. This alignment is essential for the catalysis of peptide bond formation between amino acids allowing for the accurate synthesis of proteins.

Pathways

RPL23A is integral to the mTOR signaling pathway and the p53 signaling pathway. The mTOR pathway regulates cell growth proliferation and survival in response to nutrients growth factors and cellular energy levels with RPL23A interacting with proteins like TSC1 and TSC2. In the p53 signaling pathway RPL23A can influence cellular responses to DNA damage and stress by interacting with proteins such as MDM2.

RPL23A is implicated in cancer and Diamond-Blackfan anemia. In cancer the dysregulation of RPL23A can affect ribosome biogenesis and cell proliferation often seen in conjunction with increased levels of proteins like c-Myc. In Diamond-Blackfan anemia mutations or malfunctions in RPL23A lead to defective ribosomal function impacting erythroid progenitor cells and causing anemia with links to other ribosomal proteins such as RPS19.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the large ribosomal subunit (PubMed : 23636399, PubMed : 32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399, PubMed : 32669547). Binds a specific region on the 26S rRNA (PubMed : 23636399, PubMed : 32669547). May promote p53/TP53 degradation possibly through the stimulation of MDM2-mediated TP53 polyubiquitination (PubMed : 26203195).

See full target information 60S ribosomal protein L23a

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 5:101552 PubMed38729158

2024

Inhibition of protein translational machinery in triple-negative breast cancer as a promising therapeutic strategy.

Applications

Unspecified application

Species

Unspecified reactive species

Arpit Dheeraj,Fernando Jose Garcia Marques,Dhanir Tailor,Abel Bermudez,Angel Resendez,Mallesh Pandrala,Benedikt Grau,Praveen Kumar,Carrsyn B Haley,Alexander Honkala,Praveen Kujur,Stefanie S Jeffrey,Sharon Pitteri,Sanjay V Malhotra

Cancer discovery 13:332-347 PubMed36259929

2022

A Dynamic rRNA Ribomethylome Drives Stemness in Acute Myeloid Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Fengbiao Zhou,Nesrine Aroua,Yi Liu,Christian Rohde,Jingdong Cheng,Anna-Katharina Wirth,Daria Fijalkowska,Stefanie Göllner,Michelle Lotze,Haiyang Yun,Xiaobing Yu,Caroline Pabst,Tim Sauer,Thomas Oellerich,Hubert Serve,Christoph Röllig,Martin Bornhäuser,Christian Thiede,Claudia Baldus,Michaela Frye,Simon Raffel,Jeroen Krijgsveld,Irmela Jeremias,Roland Beckmann,Andreas Trumpp,Carsten Müller-Tidow

The Plant cell 27:1389-408 PubMed25944100

2015

Autophagic recycling plays a central role in maize nitrogen remobilization.

Applications

Species

Unspecified reactive species

Faqiang Li,Taijoon Chung,Janice G Pennington,Maria L Federico,Heidi F Kaeppler,Shawn M Kaeppler,Marisa S Otegui,Richard D Vierstra

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-RPL23A antibody

Immunoprecipitation - Anti-RPL23A antibody (AB157110)

Western blot - Anti-RPL23A antibody (AB157110)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise