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AB126172

Anti-RPL24 antibody

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(2 Publications)

Rabbit Polyclonal RPL24 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human 60S ribosomal protein L24 aa 1-150.

View Alternative Names

Large ribosomal subunit protein eL24, 60S ribosomal protein L24, 60S ribosomal protein L30, RPL24

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-RPL24 antibody (AB126172)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RPL24 antibody (AB126172)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling RPL24 protein at cytoplasm using ab126172 at a 1/200 dilution (Green).
alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at a 1/1000 dilution (Red). DAPI was used for nuclear staining (Blue).

Western blot - Anti-RPL24 antibody (AB126172)
  • WB

Supplier Data

Western blot - Anti-RPL24 antibody (AB126172)

Samples were separated by 15% SDS PAGE.

All lanes:

Western blot - Anti-RPL24 antibody (ab126172) at 1/1000 dilution

Lane 1:

A549 whole cell extracts at 30 µg

Lane 2:

H1299 whole cell extracts at 30 µg

Lane 3:

HCT116 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 18 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human 60S ribosomal protein L24 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

P83731

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPL24 also known as Ribosomal Protein L24 functions as part of the 60S large ribosomal subunit. It plays an important role in the structural stability and assembly of ribosomes supporting protein synthesis within the cell. RPL24 has a molecular mass of approximately 17 kDa and exhibits high expression in actively dividing cells like those in the bone marrow and the liver. Its expression is important for the efficient production of ribosomal proteins and cellular growth.
Biological function summary

The RPL24 protein assists in the translation process by joining with other ribosomal proteins and rRNA components to form the large subunit of the ribosome. It integrates within the ribosome complex ensuring proper alignment of mRNA and tRNA during protein synthesis. RPL24 contributes to the efficiency and accuracy of translating genetic codes into functional proteins which is essential for cellular functioning. It collaborates closely with other ribosomal proteins in translation initiation and elongation processes.

Pathways

RPL24 plays an integral role in the mTOR signaling pathway which regulates cell growth protein synthesis and metabolism. This pathway heavily relies on functional ribosomes where RPL24 assists in ribosome biogenesis and activity. Another important pathway is the translation initiation pathway where RPL24 works alongside proteins like eIF3 and eIF6 ensuring the proper assembly and function of the ribosome. By participating in these pathways RPL24 supports cellular growth and maintenance.

Improper function or mutation of RPL24 associates with Diamond-Blackfan Anemia a disorder characterized by a failure to produce red blood cells. The disorder links RPL24 to reduced ribosomal function and impaired erythropoiesis. RPL24 also connects to cancers where dysregulation in ribosomal proteins often results in uncontrolled cell growth and proliferation. Mutated ribosomal proteins like those in RPL24 can alter pathways leading to oncogenesis and cancer progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.
See full target information 60S ribosomal protein L24
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 38:110597 PubMed35354024

2022

Labeling of heterochronic ribosomes reveals C1ORF109 and SPATA5 control a late step in human ribosome assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyang Ni,Daniel A Schmitz,Jeon Lee,Krzysztof Pawłowski,Jun Wu,Michael Buszczak

Nucleic acids research 45:11725-11742 PubMed28981686

2017

The nuclear mitotic apparatus protein NuMA controls rDNA transcription and mediates the nucleolar stress response in a p53-independent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Swaathi Jayaraman,Shirisha Chittiboyina,Yunfeng Bai,Patricia C Abad,Pierre-Alexandre Vidi,Cynthia V Stauffacher,Sophie A Lelièvre
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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