Rabbit Polyclonal RPL27/RPL27A antibody. Suitable for WB and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human RPL27 aa 50 to C-terminus.
View Alternative Names
Large ribosomal subunit protein eL27, 60S ribosomal protein L27, RPL27
- WB
Supplier Data
Western blot - Anti-RPL27/RPL27A antibody (AB241409)
Prepared using NETN lysis buffer.
All lanes:
Western blot - Anti-RPL27/RPL27A antibody (ab241409) at 0.1 µg/mL
Lane 1:
HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2:
HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 3:
Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 4:
TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate at 50 µg
Lane 5:
NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Predicted band size: 16 kDa
true
Exposure time: 10s
Reactivity data
Properties and storage information
Form
Purification technique
Purification notes
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
RPL27 and RPL27A function as essential components of the ribosomal machinery that drives protein translation. Both proteins contribute to ribosome biogenesis an important process in cellular protein synthesis. As part of the large 60S ribosomal subunit they participate in the formation and stabilization of the ribosomal structure enabling efficient mRNA translation. These ribosomal proteins work within a complex that includes other ribosomal proteins and ribosomal RNA (rRNA) to facilitate the synthesis of proteins necessary for cell growth and function.
Pathways
RPL27 and RPL27A play significant roles in the translation pathway an essential part of gene expression. They are involved in the initiation elongation and termination phases of protein synthesis. The function of these proteins parallels that of other ribosomal proteins like RPL23 which helps in joining amino acids to form polypeptides. Additionally RPL27 and RPL27A integrate into pathways managing cellular responses to stress by regulating the production of proteins under different conditions such as during nutrient availability or cell proliferation signaling.
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