AB74731

Anti-RPL27A antibody

Name: Anti-RPL27A antibody (ab74731) | Abcam
Brand: Abcam Limited
SKU: AB74731
Price: 525 USD
Availability: InStock
Rating: 4 (4 reviews)

(4 Reviews)

(6 Publications)

Rabbit Polyclonal RPL27A antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human RPL27A.

View Alternative Names

Large ribosomal subunit protein uL15, 60S ribosomal protein L27a, RPL27A

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-RPL27A antibody (AB74731)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RPL27A antibody (AB74731)

Immunofluorescence analysis of A549 cells, using ab74731 at 1/500 dilution with (+) or without (-) immunising peptide.

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Western blot - Anti-RPL27A antibody (AB74731)

All lanes:

Western blot - Anti-RPL27A antibody (ab74731) at 1/500 dilution

Lane 1:

extracts from COLO cells at 30 µg

Lane 2:

extracts from COLO cells at 30 µg with immunising peptide

Predicted band size: 17 kDa

Observed band size: 19 kDa,38 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human RPL27A. The exact immunogen used to generate this antibody is proprietary information.

P46776

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPL27A also known as ribosomal protein L27a is a component of the 60S large ribosomal subunit. It has a molecular weight of approximately 17 kDa and is highly conserved across species. RPL27A plays an important role in protein synthesis by facilitating the assembly of ribosomes. The protein exhibits widespread expression in various tissues reflecting its essential function in maintaining cellular processes.

Biological function summary

The function of RPL27A is to support the structural stability of ribosomes. It contributes to the formation of the ribosomal complex which is pivotal for translation. This protein interacts closely with other ribosomal proteins and ribosomal RNA to ensure accurate mRNA decoding and peptide bond formation. The integrity of the ribosomal complex is dependent on RPL27A highlighting its importance in cellular growth.

Pathways

The involvement of RPL27A in the ribosome biogenesis and protein synthesis pathways is significant. It plays a role in orchestrating the translation process where it works alongside proteins like RPL10 and RPL5. These pathways are essential for normal cell proliferation and function supporting various cellular activities and responses.

RPL27A has associations with disorders such as Diamond-Blackfan anemia and certain cancers. Mutations or dysregulation in RPL27A can impair ribosome function leading to anemia due to bone marrow failure and can contribute to tumorigenesis by affecting cell growth. In Diamond-Blackfan anemia RPL27A interacts with other defective ribosomal proteins hinting at a potential pathway disruption that leads to the disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the large ribosomal subunit (PubMed : 23636399, PubMed : 32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399, PubMed : 32669547).

See full target information RPL27A

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 41:33 PubMed35073964

2022

Loss of RPS27a expression regulates the cell cycle, apoptosis, and proliferation via the RPL11-MDM2-p53 pathway in lung adenocarcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyan Li,Hong Zhang,Guomin Huang,Zhitong Bing,Duling Xu,Jiadi Liu,Hongtao Luo,Xiaoli An

Journal of medicinal chemistry 64:17031-17050 PubMed34843649

2021

First-in-Class Inhibitors of the Ribosomal Oxygenase MINA53.

Applications

Unspecified application

Species

Unspecified reactive species

Radosław P Nowak,Anthony Tumber,Eline Hendrix,Mohammad Salik Zeya Ansari,Manuela Sabatino,Lorenzo Antonini,Regina Andrijes,Eidarus Salah,Nicola Mautone,Francesca Romana Pellegrini,Klemensas Simelis,Akane Kawamura,Catrine Johansson,Daniela Passeri,Roberto Pellicciari,Alessia Ciogli,Donatella Del Bufalo,Rino Ragno,Mathew L Coleman,Daniela Trisciuoglio,Antonello Mai,Udo Oppermann,Christopher J Schofield,Dante Rotili

Cell death and differentiation 26:1346-1364 PubMed30341421

2018

Circular RNA circAGO2 drives cancer progression through facilitating HuR-repressed functions of AGO2-miRNA complexes.

Applications

Unspecified application

Species

Unspecified reactive species

Yajun Chen,Feng Yang,Erhu Fang,Wenjing Xiao,Hong Mei,Huanhuan Li,Dan Li,Huajie Song,Jianqun Wang,Mei Hong,Xiaojing Wang,Kai Huang,Liduan Zheng,Qiangsong Tong

Cell death & disease 6:e1603 PubMed25611378

2015

The malignant phenotype in breast cancer is driven by eIF4A1-mediated changes in the translational landscape.

Applications

Species

Human

A Modelska,E Turro,R Russell,J Beaton,T Sbarrato,K Spriggs,J Miller,S Gräf,E Provenzano,F Blows,P Pharoah,C Caldas,J Le Quesne

Journal of cell science 126:3782-95 PubMed23750012

2013

Enhancement of c-Myc degradation by BLM helicase leads to delayed tumor initiation.

Applications

Unspecified application

Species

Unspecified reactive species

Suruchika Chandra,Raina Priyadarshini,Vinoth Madhavan,Shweta Tikoo,Mansoor Hussain,Richa Mudgal,Priyanka Modi,Vivek Srivastava,Sagar Sengupta

BMC molecular biology 13:9 PubMed22436005

2012

Novel polysome messages and changes in translational activity appear after induction of adipogenesis in 3T3-L1 cells.

Applications

Species

Unspecified reactive species

Carolin Fromm-Dornieden,Silvia von der Heyde,Oleksandr Lytovchenko,Gabriela Salinas-Riester,Bertram Brenig,Tim Beissbarth,Bernhard G Baumgartner

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPL27A antibody

Immunocytochemistry/ Immunofluorescence - Anti-RPL27A antibody (AB74731)

Western blot - Anti-RPL27A antibody (AB74731)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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