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AB138125

Anti-RPL28 antibody

4

(2 Reviews)

|

(6 Publications)

Rabbit Polyclonal RPL28 antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human RPL28.

View Alternative Names

Large ribosomal subunit protein eL28, 60S ribosomal protein L28, RPL28

1 Images
Western blot - Anti-RPL28 antibody (AB138125)
  • WB

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Western blot - Anti-RPL28 antibody (AB138125)

All lanes:

Western blot - Anti-RPL28 antibody (ab138125) at 1/500 dilution

All lanes:

HeLa cell lysate at 30 µg

Predicted band size: 16 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human RPL28. The exact immunogen used to generate this antibody is proprietary information.

P46779

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab138125 was affinity-purified from rabbit antiserum by immunogenic peptide affinity-chromatography.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ribosomal protein L28 (RPL28) also known as 60S ribosomal protein L28 plays a mechanical role in the formation of ribosomes which are essential for protein synthesis. RPL28 forms part of the large 60S subunit of the ribosome. This protein has a molecular mass of approximately 15-17 kDa and is expressed in various tissues due to its fundamental role in cellular functions. The expression of RPL28 occurs widely but can change in response to cellular activity related to protein production needs.
Biological function summary

RPL28 participates in the assembly and function of ribosomes within the cell. It acts as a structural component of the ribosomal large subunit and therefore helps in the synthesis of proteins by translating mRNA. As part of the ribosomal complex RPL28 ensures the proper alignment of rRNA and tRNA during protein assembly. Its function is integral to the ribosome’s role in facilitating correct translation steps needed for cellular protein production.

Pathways

RPL28 has important roles in the protein synthesis (translation) and ribosome biogenesis pathways. It interacts with other ribosomal proteins and factors involved in these pathways. RPL28 coordinates with proteins such as RPL5 and RPL11 which are also involved in the assembly of the large ribosomal subunit and ensure effective translation and synthesis of proteins required by the cell.

Mutations or alterations in RPL28 expression have connections to disorders of ribosome dysfunction known as ribosomopathies. Such conditions may include Diamond-Blackfan Anemia where ribosomal biogenesis is impaired possibly involving other proteins like RPL5. Deficiencies in RPL28 or associated proteins can lead to abnormal ribosome assembly affecting protein synthesis and contributing to disease symptoms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the large ribosomal subunit (PubMed : 12962325, PubMed : 23636399, PubMed : 32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 12962325, PubMed : 23636399, PubMed : 32669547).
See full target information RPL28
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Nature cell biology 27:1240-1255 PubMed40760247

2025

A programmed decline in ribosome levels governs human early neurodevelopment.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyang Ni,Yudong Wei,Barbara Vona,Dayea Park,Yulei Wei,Daniel A Schmitz,Yi Ding,Masahiro Sakurai,Emily Ballard,Leijie Li,Yan Liu,Ashwani Kumar,Chao Xing,Shenlu Qin,Sangin Kim,Martina Foglizzo,Jianchao Zhao,Hyung-Goo Kim,Cumhur Ekmekci,Ehsan Ghayoor Karimiani,Shima Imannezhad,Fatemeh Eghbal,Reza Shervin Badv,Eva Maria Christina Schwaibold,Mohammadreza Dehghani,Mohammad Yahya Vahidi Mehrjardi,Zahra Metanat,Hosein Eslamiyeh,Ebtissal Khouj,Saleh Mohammed Nasser Alhajj,Aziza Chedrawi,Khushnooda Ramzan,Jamil A Hashmi,Majed M Alluqmani,Sulman Basit,Danai Veltra,Nikolaos M Marinakis,Georgios Niotakis,Pelagia Vorgia,Christalena Sofocleous,Hane Lee,Won Chan Jeong,Muhammad Umair,Muhammad Bilal,César Augusto Pinheiro Ferreira Alves,Matthew Sieber,Michael Kruer,Henry Houlden,Fowzan S Alkuraya,Elton Zeqiraj,Roger A Greenberg,Can Cenik,Leqian Yu,Reza Maroofian,Jun Wu,Michael Buszczak

eLife 12: PubMed38512721

2024

Paradoxical imbalance between activated lymphocyte protein synthesis capacity and rapid division rate.

Applications

Unspecified application

Species

Unspecified reactive species

Mina O Seedhom,Devin Dersh,Jaroslav Holly,Mariana Pavon-Eternod,Jiajie Wei,Matthew Angel,Lucas Shores,Alexandre David,Jefferson Santos,Heather Hickman,Jonathan W Yewdell

Nature 583:303-309 PubMed32612236

2020

Systematic quantitative analysis of ribosome inventory during nutrient stress.

Applications

Unspecified application

Species

Unspecified reactive species

Heeseon An,Alban Ordureau,Maria Körner,Joao A Paulo,J Wade Harper

Genome biology 21:140 PubMed32539747

2020

Dynamic rewiring of the human interactome by interferon signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Craig H Kerr,Michael A Skinnider,Daniel D T Andrews,Angel M Madero,Queenie W T Chan,R Greg Stacey,Nikolay Stoynov,Eric Jan,Leonard J Foster

Molecular cell 73:1162-1173.e5 PubMed30712990

2019

Ribosomal Proteins Regulate MHC Class I Peptide Generation for Immunosurveillance.

Applications

Unspecified application

Species

Unspecified reactive species

Jiajie Wei,Rigel J Kishton,Matthew Angel,Crystal S Conn,Nicole Dalla-Venezia,Virginie Marcel,Anne Vincent,Frédéric Catez,Sabrina Ferré,Lilia Ayadi,Virginie Marchand,Devin Dersh,James S Gibbs,Ivaylo P Ivanov,Nathan Fridlyand,Yohann Couté,Jean-Jacques Diaz,Shu-Bing Qian,Louis M Staudt,Nicholas P Restifo,Jonathan W Yewdell

Nature cell biology 20:135-143 PubMed29230017

2017

Systematic analysis of ribophagy in human cells reveals bystander flux during selective autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Heeseon An,J Wade Harper
View all publications
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