Rabbit Polyclonal RPL3 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RPL3.
View Alternative Names
OK/SW-cl.32, RPL3, Large ribosomal subunit protein uL3, 60S ribosomal protein L3, HIV-1 TAR RNA-binding protein B, TARBP-B
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-RPL3 antibody (AB228638)
Methanol-fixed A431 (human epidermoid carcinoma cell line) cells stained for RPL3 (green) using ab228638 at 1/200 dilution in ICC/IF.
Nuclear counterstain : Hoechst 33342.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL3 antibody (AB228638)
Paraffin-embedded mouse intestine tissue stained for RPL3 with ab228638 at 1/500 dilution in immunohistochemical analysis.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL3 antibody (AB228638)
Paraffin-embedded mouse brain tissue stained for RPL3 with ab228638 at 1/500 dilution in immunohistochemical analysis.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL3 antibody (AB228638)
Paraffin-embedded 4T1 xenograft tissue stained for RPL3 with ab228638 at 1/500 dilution in immunohistochemical analysis.
- WB
Supplier Data
Western blot - Anti-RPL3 antibody (AB228638)
10% SDS-PAGE
All lanes:
Western blot - Anti-RPL3 antibody (ab228638) at 1/1000 dilution
Lane 1:
Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 30 µg
Lane 2:
Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg
Secondary
All lanes:
HRP-conjugated anti-rabbit IgG
Predicted band size: 46 kDa
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Reactivity data
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Supplementary information
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Biological function summary
RPL3 is integral to the formation of the ribosome a large molecular complex essential for translating mRNA into protein chains. As a component of the ribosomal 60S subunit RPL3 associates with other ribosomal proteins and ribosomal RNA to ensure correct ribosome assembly and function. It is involved in the peptidyl transferase activity of the ribosome directly facilitating peptide bond formation between amino acids.
Pathways
The ribosomal protein function of RPL3 is an important part of the translational machinery involved in the mTOR signaling pathway and the cellular response to amino acid availability. RPL3 functioning within the ribosome works alongside proteins such as RPL5 and RPL10 which also participate in these pathways. This protein's involvement ensures efficient protein synthesis according to cellular demands and environmental signals impacting cellular metabolism and growth.
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Target data
Publications (2)
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Neuron 111:2881-2898.e12 PubMed37442131
2023
Applications
Unspecified application
Species
Unspecified reactive species
Journal of molecular histology 52:1067-1080 PubMed34398360
2021
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
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