Rabbit Polyclonal RPL3 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human RPL3 aa 300-400.
View Alternative Names
OK/SW-cl.32, RPL3, Large ribosomal subunit protein uL3, 60S ribosomal protein L3, HIV-1 TAR RNA-binding protein B, TARBP-B
- IP
Supplier Data
Immunoprecipitation - Anti-RPL3 antibody (AB241412)
RPL3 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241412 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab241412 at 0.4 μg/ml.
Lane 1 : ab241412 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.
Detection : Chemiluminescence with exposure time of 30 seconds.
Lysates prepared using NETN lysis buffer.
All lanes:
Immunoprecipitation - Anti-RPL3 antibody (ab241412)
Predicted band size: 46 kDa
false
- WB
Supplier Data
Western blot - Anti-RPL3 antibody (AB241412)
Lysates prepared using NETN lysis buffer.
All lanes:
Western blot - Anti-RPL3 antibody (ab241412) at 0.4 µg/mL
Lane 1:
HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2:
HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 3:
Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 4:
TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg
Lane 5:
NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate at 50 µg
Predicted band size: 46 kDa
true
Exposure time: 30s
Reactivity data
Properties and storage information
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Purification technique
Purification notes
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
RPL3 is integral to the formation of the ribosome a large molecular complex essential for translating mRNA into protein chains. As a component of the ribosomal 60S subunit RPL3 associates with other ribosomal proteins and ribosomal RNA to ensure correct ribosome assembly and function. It is involved in the peptidyl transferase activity of the ribosome directly facilitating peptide bond formation between amino acids.
Pathways
The ribosomal protein function of RPL3 is an important part of the translational machinery involved in the mTOR signaling pathway and the cellular response to amino acid availability. RPL3 functioning within the ribosome works alongside proteins such as RPL5 and RPL10 which also participate in these pathways. This protein's involvement ensures efficient protein synthesis according to cellular demands and environmental signals impacting cellular metabolism and growth.
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Target data
Publications (3)
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Molecular medicine reports 28: PubMed37326104
2023
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Unspecified application
Species
Unspecified reactive species
Communications biology 5:564 PubMed35681048
2022
Applications
Unspecified application
Species
Unspecified reactive species
Nature communications 12:1334 PubMed33637765
2021
Applications
Unspecified application
Species
Unspecified reactive species
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