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AB249601

Anti-RPL30 antibody [EPR11624] - BSA and Azide free

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Rabbit Recombinant Monoclonal RPL30 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB and reacts with Human samples.

View Alternative Names

Large ribosomal subunit protein eL30, 60S ribosomal protein L30, RPL30

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-RPL30 antibody [EPR11624] - BSA and Azide free (AB249601)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RPL30 antibody [EPR11624] - BSA and Azide free (AB249601)

This data was developed using ab170930, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of HepG2 cells labeling RPL30 with ab170930 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL30 antibody [EPR11624] - BSA and Azide free (AB249601)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL30 antibody [EPR11624] - BSA and Azide free (AB249601)

This data was developed using ab170930, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling RPL30 with ab170930 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL30 antibody [EPR11624] - BSA and Azide free (AB249601)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL30 antibody [EPR11624] - BSA and Azide free (AB249601)

This data was developed using ab170930, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling RPL30 with ab170930 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-RPL30 antibody [EPR11624] - BSA and Azide free (AB249601)
  • IP

Supplier Data

Immunoprecipitation - Anti-RPL30 antibody [EPR11624] - BSA and Azide free (AB249601)

This data was developed using ab170930, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate using ab170930 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-RPL30 antibody [EPR11624] (<a href='/en-us/products/primary-antibodies/rpl30-antibody-epr11624-ab170930'>ab170930</a>)

Predicted band size: 13 kDa

false

Western blot - Anti-RPL30 antibody [EPR11624] - BSA and Azide free (AB249601)
  • WB

Supplier Data

Western blot - Anti-RPL30 antibody [EPR11624] - BSA and Azide free (AB249601)

This data was developed using ab170930, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RPL30 antibody [EPR11624] (<a href='/en-us/products/primary-antibodies/rpl30-antibody-epr11624-ab170930'>ab170930</a>) at 1/10000 dilution

Lane 1:

A549 cell lysates at 10 µg

Lane 2:

HepG2 cell lysates at 10 µg

Lane 3:

Jurkat cell lysates at 10 µg

Lane 4:

PC-3 cell lysates at 10 µg

Predicted band size: 13 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11624

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF, IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab249601 is the carrier-free version of ab170930.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPL30 also known as 60S ribosomal protein L30 is a component of the large 60S subunit of the ribosome. It has a molecular mass of approximately 12 kDa and is commonly expressed in all cells due to its essential role in protein synthesis. RPL30 functions mechanically by interacting with ribosomal RNA (rRNA) and other ribosomal proteins to form the ribosome structure ensuring proper translation of mRNA into proteins.
Biological function summary

Ribosomal protein L30 participates in the assembly of the ribosome complex which is responsible for cellular protein production. It plays a critical part in the ribosomal structure contributing to the ribosome's stability and function during translation. By binding to rRNA RPL30 ensures accurate alignment of ribosomal components supporting efficient and accurate protein synthesis. The protein is highly conserved and is ubiquitously expressed across various tissues and cell types due to its fundamental role.

Pathways

RPL30 is integral to the ribosome biogenesis and protein synthesis pathways. The protein interacts with other ribosomal proteins such as RPL10 and RPL5 to facilitate the assembly and function of the ribosome. These pathways are essential for cellular growth and proliferation influencing various biological processes including metabolism and cell cycle regulation. RPL30 works within these pathways to aid in the coordination of protein synthesis an activity critical for maintaining cellular homeostasis.

RPL30 is associated with diseases affecting protein synthesis such as ribosomopathies. Alterations or mutations in RPL30 can lead to defects in ribosome function contributing to conditions like Diamond-Blackfan anemia. This disorder is characterized by a failure in red blood cell production which can be linked to mutations in ribosomal proteins including RPS19. The relationship between RPL30 and these diseases highlights the importance of accurate ribosome function for maintaining normal cellular activities.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the large ribosomal subunit (PubMed : 23636399, PubMed : 32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399, PubMed : 32669547).
See full target information 60S ribosomal protein L30
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Product promise

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For full details, please see our Terms & Conditions

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