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AB229758

Anti-RPL32 antibody

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(2 Publications)

Rabbit Polyclonal RPL32 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RPL32.

View Alternative Names

PP9932, RPL32, Large ribosomal subunit protein eL32, 60S ribosomal protein L32

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-RPL32 antibody (AB229758)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RPL32 antibody (AB229758)

4% paraformaldehyde-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for RPL32 (green) using ab229758 at 1/1000 dilution in ICC/IF.

Nuclear counterstain : Hoechst 33342 (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL32 antibody (AB229758)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL32 antibody (AB229758)

Paraffin-embedded human lung cancer tissue stained for RPL32 with ab229758 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL32 antibody (AB229758)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL32 antibody (AB229758)

Paraffin-embedded human colon cancer tissue stained for RPL32 with ab229758 at 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-RPL32 antibody (AB229758)
  • WB

Supplier Data

Western blot - Anti-RPL32 antibody (AB229758)

Samples were separated by 15% SDS PAGE.

All lanes:

Western blot - Anti-RPL32 antibody (ab229758) at 1/1000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 16 kDa

false

Western blot - Anti-RPL32 antibody (AB229758)
  • WB

Supplier Data

Western blot - Anti-RPL32 antibody (AB229758)

Samples were separated by 15% SDS PAGE.

All lanes:

Western blot - Anti-RPL32 antibody (ab229758) at 1/1000 dilution

Lane 1:

Mouse testis tissue extracts at 50 µg

Lane 2:

Rat testis tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 16 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human RPL32.

P62910

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPL32 also known as 60S ribosomal protein L32 is a component of the 60S large ribosomal subunit. Weighing approximately 13.5 kDa RPL32 plays a role in the assembly and function of ribosomes which are essential for protein synthesis. This protein is expressed ubiquitously in various tissues reflecting the constant need for ribosomal function across different cell types.
Biological function summary

Ribosomes are the machinery for translating mRNA into proteins in the cell. RPL32 forms part of the ribosomal complex engaging in the critical process of assembling amino acids into polypeptide chains. By contributing to ribosome stability and efficiency RPL32 ensures the fidelity and throughput of protein synthesis which is important for cellular growth and maintenance.

Pathways

Protein synthesis involves ribosome-mediated translation where RPL32 acts in coordination with other ribosomal proteins and rRNA. It integrates into the translation pathway working with key proteins like RPL10 and RPL5 to facilitate ribosomal activity. The interaction of these proteins influences the performance of ribosomes in synthesizing functional proteins needed for cellular processes including metabolism and stress response.

Disruptions in RPL32 function can lead to disease states connected to dysregulated protein synthesis. Research implicates RPL32 in certain malignancies particularly where ribosomal dysfunction contributes to unchecked cellular proliferation. Moreover RPL32 abnormalities show correlation with disorders like Diamond-Blackfan anemia which involves defective ribosomal function. The protein's relationship with other ribosomal constituents such as RPL24 suggests a complex network of interactions that can impact disease outcomes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the large ribosomal subunit (PubMed : 23636399, PubMed : 32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399, PubMed : 32669547).
See full target information RPL32
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of molecular biology 436:168434 PubMed38182103

2024

KH-like Domains in PARP9/DTX3L and PARP14 Coordinate Protein-Protein Interactions to Promote Cancer Cell Survival.

Applications

Unspecified application

Species

Unspecified reactive species

Hadil Saleh,Triantafillos Liloglou,Daniel J Rigden,Jason L Parsons,Gabrielle J Grundy

Molecular medicine reports 22:2478-2486 PubMed32705264

2020

Biological effect of ribosomal protein L32 on human breast cancer cell behavior.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Xu,Lintao Wang,Chaojun Jiang,Qiannan Zhu,Rui Chen,Jue Wang,Shui Wang
View all publications
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Product promise

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