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AB121244

Anti-RPL35 antibody

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(2 Publications)

Rabbit Polyclonal RPL35 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human 60S ribosomal protein L35 aa 1 to C-terminus.

View Alternative Names

Large ribosomal subunit protein uL29, 60S ribosomal protein L35, RPL35

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL35 antibody (AB121244)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL35 antibody (AB121244)

Immunohistochemical analysis of human cerebral fallopian tube labeling rpl35 with ab121244. The data shows moderate cytoplasmic positivity in glandular cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL35 antibody (AB121244)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL35 antibody (AB121244)

Immunohistochemical analysis of human cerebral colon labeling rpl35 with ab121244. The data shows moderate cytoplasmic positivity in glandular cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL35 antibody (AB121244)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL35 antibody (AB121244)

Immunohistochemical analysis of human skin tissue labeling rpl35 with ab121244. The data shows weak staining in squamous epithelial cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL35 antibody (AB121244)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL35 antibody (AB121244)

Immunohistochemical analysis of human cerebral cortex tissue labeling rpl35 with ab121244. The data shows moderate cytoplasmic positivity in neurons.

Immunocytochemistry/ Immunofluorescence - Anti-RPL35 antibody (AB121244)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RPL35 antibody (AB121244)

Immunofluorescent staining with 4 µg/ml ab121244 of Human cell line U-2 OS shows positivity in cytoplasm. Antibody staining is shown in green.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL35 antibody (AB121244)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL35 antibody (AB121244)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling RPL35 with ab121244 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-RPL35 antibody (AB121244)
  • WB

Supplier Data

Western blot - Anti-RPL35 antibody (AB121244)

All lanes:

Western blot - Anti-RPL35 antibody (ab121244) at 0.4 µg/mL

All lanes:

MCF7 cell lysate

Predicted band size: 15 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human 60S ribosomal protein L35 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P42766

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPL35 also known as Ribosomal Protein L35 plays an essential role in ribosome function. It is a component of the large 60S ribosomal subunit and has a molecular mass of around 13.4 kDa. RPL35 exhibits widespread expression in human tissues suggesting it is necessary for general protein synthesis functions across different cell types.
Biological function summary

The function of RPL35 contributes to the formation and stability of the ribosome which is a complex structure composed of ribosomal RNA and ribosomal proteins. RPL35's integration into the ribosome assists in the assembly of new proteins by ensuring correct translation. This integration is necessary for maintaining the efficiency and accuracy of protein synthesis within cells playing an important role in cell growth and proliferation.

Pathways

The activity of RPL35 influences the mRNA translation pathway and connects closely to the protein biosynthesis process. Through its interaction with the ribosomal machinery it also relates to the mTOR signaling pathway which is significant for cell growth regulation. Proteins like RPLP0 and RPLP1 are associated with RPL35 within these pathways supporting a robust translation and protein synthesis network.

Mutations and dysregulation of RPL35 relate to disorders like Diamond-Blackfan anemia and some cancers. These conditions link to the disruption in ribosome biogenesis or function which leads to faulty protein synthesis. In Diamond-Blackfan anemia abnormal function of RPL35 along with other ribosomal proteins such as RPL5 disrupts normal erythropoiesis resulting in anemia.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the large ribosomal subunit (PubMed : 12962325, PubMed : 23636399, PubMed : 32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 12962325, PubMed : 23636399, PubMed : 32669547).
See full target information 60S ribosomal protein L35
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

PeerJ 8:e9629 PubMed32821546

2020

Spermatogenesis is normal in knockout mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zhendong Zhu,Xin Zhang,Wentao Zeng,Shuqin Zhao,Jianli Zhou,Zuomin Zhou,Mingxi Liu

Nature communications 10:5026 PubMed31690716

2019

The long noncoding RNA lncNB1 promotes tumorigenesis by interacting with ribosomal protein RPL35.

Applications

Unspecified application

Species

Unspecified reactive species

Pei Y Liu,Andrew E Tee,Giorgio Milazzo,Katherine M Hannan,Jesper Maag,Sujanna Mondal,Bernard Atmadibrata,Nenad Bartonicek,Hui Peng,Nicholas Ho,Chelsea Mayoh,Roberto Ciaccio,Yuting Sun,Michelle J Henderson,Jixuan Gao,Celine Everaert,Amy J Hulme,Matthew Wong,Qing Lan,Belamy B Cheung,Leming Shi,Jenny Y Wang,Thorsten Simon,Matthias Fischer,Xu D Zhang,Glenn M Marshall,Murray D Norris,Michelle Haber,Jo Vandesompele,Jinyan Li,Pieter Mestdagh,Ross D Hannan,Marcel E Dinger,Giovanni Perini,Tao Liu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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