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AB190162

Anti-RPL35 antibody

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(3 Publications)

Rabbit Polyclonal RPL35 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human 60S ribosomal protein L35 aa 50-100.

View Alternative Names

Large ribosomal subunit protein uL29, 60S ribosomal protein L35, RPL35

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL35 antibody (AB190162)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL35 antibody (AB190162)

Immunohistochemical staining of Human brain cortex tissue labeling RPL35 using ab190162 at 10 μg/ml.

Western blot - Anti-RPL35 antibody (AB190162)
  • WB

Supplier Data

Western blot - Anti-RPL35 antibody (AB190162)

All lanes:

Western blot - Anti-RPL35 antibody (ab190162) at 1/500 dilution

Lane 1:

HeLa cell lysate

Lane 2:

Jurkat cell lysate

Lane 3:

COLO cell lysate

Lane 4:

293 cell lysate

Lane 5:

HeLa cell lysate with Synthesized peptide

Predicted band size: 15 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human 60S ribosomal protein L35 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

P42766

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPL35 also known as Ribosomal Protein L35 plays an essential role in ribosome function. It is a component of the large 60S ribosomal subunit and has a molecular mass of around 13.4 kDa. RPL35 exhibits widespread expression in human tissues suggesting it is necessary for general protein synthesis functions across different cell types.
Biological function summary

The function of RPL35 contributes to the formation and stability of the ribosome which is a complex structure composed of ribosomal RNA and ribosomal proteins. RPL35's integration into the ribosome assists in the assembly of new proteins by ensuring correct translation. This integration is necessary for maintaining the efficiency and accuracy of protein synthesis within cells playing an important role in cell growth and proliferation.

Pathways

The activity of RPL35 influences the mRNA translation pathway and connects closely to the protein biosynthesis process. Through its interaction with the ribosomal machinery it also relates to the mTOR signaling pathway which is significant for cell growth regulation. Proteins like RPLP0 and RPLP1 are associated with RPL35 within these pathways supporting a robust translation and protein synthesis network.

Mutations and dysregulation of RPL35 relate to disorders like Diamond-Blackfan anemia and some cancers. These conditions link to the disruption in ribosome biogenesis or function which leads to faulty protein synthesis. In Diamond-Blackfan anemia abnormal function of RPL35 along with other ribosomal proteins such as RPL5 disrupts normal erythropoiesis resulting in anemia.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the large ribosomal subunit (PubMed : 12962325, PubMed : 23636399, PubMed : 32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 12962325, PubMed : 23636399, PubMed : 32669547).
See full target information 60S ribosomal protein L35
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 9:453 PubMed38086802

2023

A novel microtubule inhibitor promotes tumor ferroptosis by attenuating SLC7A11/GPX4 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Nannan Ning,Ziqi Shang,Zhiping Liu,Zhizhou Xia,Yang Li,Ruibao Ren,Hongmei Wang,Yi Zhang

Experimental neurology :113594 PubMed33450233

2021

Age-related loss of axonal regeneration is reflected by the level of local translation.

Applications

Unspecified application

Species

Unspecified reactive species

Susan van Erp,Annemiek A van Berkel,Eline M Feenstra,Pabitra K Sahoo,Laura Wagstaff,Jeffery L Twiss,James W Fawcett,Richard Eva,Charles Ffrench-Constant

BioMed research international 2017:7508316 PubMed28293639

2017

Proteomic Analysis of Liver Proteins in a Rat Model of Chronic Restraint Stress-Induced Depression.

Applications

Unspecified application

Species

Unspecified reactive species

Cong Li,Zhengguang Guo,Ronghua Zhao,Wei Sun,Ming Xie
View all publications
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Product promise

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