AB241584

Anti-RPL36 antibody

Name: Anti-RPL36 antibody (ab241584) | Abcam
Brand: Abcam Limited
SKU: AB241584

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(2 Publications)

Rabbit Polyclonal RPL36 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human RPL36 aa 50 to C-terminus.

View Alternative Names

Large ribosomal subunit protein eL36, 60S ribosomal protein L36, RPL36

2 Images

Supplier Data

Immunoprecipitation - Anti-RPL36 antibody (AB241584)

RPL36 was immunoprecipitated from HeLa (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241584 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab241584 at 1 μg/ml.

Lane 1 : ab241584 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 10 seconds.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-RPL36 antibody (ab241584)

Predicted band size: 12 kDa

false

Supplier Data

Western blot - Anti-RPL36 antibody (AB241584)

Lysates prepared in NETN buffer.

All lanes:

Western blot - Anti-RPL36 antibody (ab241584) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 12 kDa

true

Exposure time: 1s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human RPL36 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y3U8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/25000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/25000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antibody was affinity purified using an epitope specific to RPL36 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPL36 also known as Ribosomal Protein L36 is a component of the 60S subunit of the eukaryotic ribosome. This protein has a mass of approximately 12.5 kDa. RPL36 helps in the assembly of ribosomes facilitating protein synthesis in the cell. It is expressed in various tissues including the liver and brain where cell growth and division require robust protein production.

Biological function summary

RPL36 plays an essential role in the synthesis of proteins by participating in ribosome biogenesis. As part of the ribosomal complex it contributes to the formation of the large ribosomal subunit which in collaboration with the small subunit synthesizes proteins from mRNA. This function places RPL36 at the heart of cellular physiology as protein synthesis drives many cellular processes.

Pathways

RPL36 is involved in the cellular process of translation. It is part of the ribosome-mediated mechanism that translates genetic information into proteins. RPL36 associates with other ribosomal proteins like RPL10 and RPL13A within the translational machinery. Additionally it is part of the mTOR signaling pathway which regulates cell growth proliferation and survival in response to nutrient availability and other signals.

RPL36 influences conditions such as Diamond-Blackfan anemia and various cancers. In Diamond-Blackfan anemia mutations affecting ribosomal proteins like RPL36 disrupt normal erythropoiesis leading to bone marrow failure. In cancer changes in ribosomal protein expression including RPL36 may contribute to abnormal protein synthesis and tumor growth. The interplay between RPL36 and other proteins such as RPL5 and RPL11 can impact pathways critical to disease development and progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the large ribosomal subunit (PubMed : 12962325, PubMed : 23636399, PubMed : 25901680, PubMed : 25957688, PubMed : 32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 12962325, PubMed : 23636399, PubMed : 25901680, PubMed : 25957688, PubMed : 32669547).

See full target information RPL36

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Disease markers 2022:5085183 PubMed36118675

2022

Curcumin Improves Keratinocyte Proliferation, Inflammation, and Oxidative Stress through Mediating the SPAG5/FOXM1 Axis in an Model of Actinic Dermatitis by Ultraviolet.

Applications

Unspecified application

Species

Unspecified reactive species

Quan Chen,Yi Tang,Huiyan Deng,Bihua Liang,Huaping Li,Zhenjie Li,Huilan Zhu,Lezi Chen

iScience 24:102700 PubMed34235408

2021

Translatomic analysis of regenerating and degenerating spinal motor neurons in injury and ALS.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer L Shadrach,Wesley M Stansberry,Allison M Milen,Rachel E Ives,Elizabeth A Fogarty,Anthony Antonellis,Brian A Pierchala

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPL36 antibody

Immunoprecipitation - Anti-RPL36 antibody (AB241584)

Western blot - Anti-RPL36 antibody (AB241584)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise