AB228542

Anti-RPL37 antibody

Name: Anti-RPL37 antibody (ab228542) | Abcam
Brand: Abcam Limited
SKU: AB228542
Price: 525 USD
Availability: InStock

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Rabbit Polyclonal RPL37 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human 60S ribosomal protein L37.

View Alternative Names

Large ribosomal subunit protein eL37, 60S ribosomal protein L37, G1.16, RPL37

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-RPL37 antibody (AB228542)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RPL37 antibody (AB228542)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for RPL37 (green) using ab228542 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Blue : Hoechst 33342.

Supplier Data

Western blot - Anti-RPL37 antibody (AB228542)

15% SDS-PAGE

All lanes:

Western blot - Anti-RPL37 antibody (ab228542) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Predicted band size: 11 kDa

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Supplier Data

Western blot - Anti-RPL37 antibody (AB228542)

15% SDS-PAGE

All lanes:

Western blot - Anti-RPL37 antibody (ab228542) at 1/1000 dilution

Lane 1:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 30 µg

Lane 2:

Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg

Lane 3:

NCI-H929 whole cell lysate at 30 µg

Predicted band size: 11 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human 60S ribosomal protein L37. The exact immunogen used to generate this antibody is proprietary information.

P61927

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPL37 also known as ribosomal protein L37 is a component of the 60S subunit of eukaryotic ribosomes. The protein possesses a molecular mass of approximately 11 kDa. It is expressed in various tissues throughout the body and plays a fundamental role in protein synthesis. RPL37 binds to rRNA and is involved in the assembly and structural stability of the ribosomal unit facilitating the translation process during protein expression.

Biological function summary

Ribosomal protein L37 is essential in the formation and function of ribosome complexes. It is part of the large ribosomal subunit that contributes to stabilizing the interactions between the rRNA and the mRNA-tRNA complex during translation. The precise positioning of these molecules by RPL37 helps ensure accurate decoding of mRNA into polypeptide chains highlighting its role in efficient protein production within cells.

Pathways

RPL37 is implicated in the intricate mechanics of the ribosomal biogenesis and translation pathways. Within these pathways it cooperates with other ribosomal proteins such as RPL5 and RPL10 which also form part of the large ribosomal subunit. Together they enable the step-wise assembly and function of ribosomes ultimately impacting cellular growth and proliferation through protein synthesis.

Mutations or dysregulation of RPL37 can relate to various conditions such as cancer and Diamond-Blackfan anemia. In cancer aberrant expression of RPL37 has been connected to altered ribosome function impacting cell cycle progression and growth. Additionally in Diamond-Blackfan anemia RPL37 mutations can associate with disturbed ribosomal biogenesis leading to insufficient erythrocyte production. Associated proteins such as RPL11 and RPL15 may also show altered expression in these conditions further complicating the cellular environment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the large ribosomal subunit (PubMed : 23636399, PubMed : 32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399, PubMed : 32669547).

See full target information 60S ribosomal protein L37

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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