Rabbit Polyclonal RPL37 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human 60S ribosomal protein L37.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human 60S ribosomal protein L37. The exact immunogen used to generate this antibody is proprietary information. Database link P61927
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Chicken | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow, Dog, Pig, Xenopus laevis, Chimpanzee | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow, Dog, Pig, Xenopus laevis, Chimpanzee | Dilution info - | Notes - |
Target data
Function
Component of the large ribosomal subunit (PubMed:23636399, PubMed:32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:23636399, PubMed:32669547).
Alternative names
Large ribosomal subunit protein eL37, 60S ribosomal protein L37, G1.16, RPL37
Recommended products
Rabbit Polyclonal RPL37 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human 60S ribosomal protein L37.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human 60S ribosomal protein L37. The exact immunogen used to generate this antibody is proprietary information. Database link P61927
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RPL37 also known as ribosomal protein L37 is a component of the 60S subunit of eukaryotic ribosomes. The protein possesses a molecular mass of approximately 11 kDa. It is expressed in various tissues throughout the body and plays a fundamental role in protein synthesis. RPL37 binds to rRNA and is involved in the assembly and structural stability of the ribosomal unit facilitating the translation process during protein expression.
- Biological function summary
Ribosomal protein L37 is essential in the formation and function of ribosome complexes. It is part of the large ribosomal subunit that contributes to stabilizing the interactions between the rRNA and the mRNA-tRNA complex during translation. The precise positioning of these molecules by RPL37 helps ensure accurate decoding of mRNA into polypeptide chains highlighting its role in efficient protein production within cells.
- Pathways
RPL37 is implicated in the intricate mechanics of the ribosomal biogenesis and translation pathways. Within these pathways it cooperates with other ribosomal proteins such as RPL5 and RPL10 which also form part of the large ribosomal subunit. Together they enable the step-wise assembly and function of ribosomes ultimately impacting cellular growth and proliferation through protein synthesis.
- Associated diseases and disorders
Mutations or dysregulation of RPL37 can relate to various conditions such as cancer and Diamond-Blackfan anemia. In cancer aberrant expression of RPL37 has been connected to altered ribosome function impacting cell cycle progression and growth. Additionally in Diamond-Blackfan anemia RPL37 mutations can associate with disturbed ribosomal biogenesis leading to insufficient erythrocyte production. Associated proteins such as RPL11 and RPL15 may also show altered expression in these conditions further complicating the cellular environment.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Immunocytochemistry/ Immunofluorescence - Anti-RPL37 antibody (ab228542)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for RPL37 (green) using ab228542 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Blue: Hoechst 33342.
Western blot - Anti-RPL37 antibody (ab228542)
15% SDS-PAGE
All lanes: Western blot - Anti-RPL37 antibody (ab228542) at 1/1000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 11 kDa
Western blot - Anti-RPL37 antibody (ab228542)
15% SDS-PAGE
All lanes: Western blot - Anti-RPL37 antibody (ab228542) at 1/1000 dilution
Lane 1: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 30 µg
Lane 2: Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg
Lane 3: NCI-H929 whole cell lysate at 30 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 11 kDa
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