AB154907

Anti-RPL4 antibody

Name: Anti-RPL4 antibody (ab154907) | Abcam
Brand: Abcam Limited
SKU: AB154907
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal RPL4 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RPL4 aa 50-350.

View Alternative Names

RPL1, RPL4, Large ribosomal subunit protein uL4, 60S ribosomal protein L1, 60S ribosomal protein L4

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL4 antibody (AB154907)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL4 antibody (AB154907)

Immunohistochemical analysis of paraffin-embedded human U87 xenograft tissue staining RPL4 with ab154907 at 1/500 dilution.

Antigen Retrieval : EDTA based buffer, pH 8.0, 15min.

Immunocytochemistry/ Immunofluorescence - Anti-RPL4 antibody (AB154907)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RPL4 antibody (AB154907)

Immunofluorescence analysis of ice-cold MeOH HepG2 (human liver hepatocellular carcinoma cell line) whole cell labeling RPL4 at cytoplasm with ab154907 at 1/500 dilution. Blue : Hoechst 33342 staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL4 antibody (AB154907)

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue staining RPL4 at cytosol with ab154907 at 1/500 dilution.

Antigen Retrieval : EDTA based buffer, pH 8.0, 15min.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL4 antibody (AB154907)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue staining RPL4 at cytosol with ab154907 at 1/500 dilution.

Antigen Retrieval : EDTA based buffer, pH 8.0, 15min.

Supplier Data

Western blot - Anti-RPL4 antibody (AB154907)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-RPL4 antibody (ab154907) at 1/1000 dilution

Lane 1:

Jurkat whole cell extracts at 30 µg

Lane 2:

Raji whole cell extracts at 30 µg

Lane 3:

NCI-H929 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 48 kDa

false

Supplier Data

Western blot - Anti-RPL4 antibody (AB154907)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-RPL4 antibody (ab154907) at 1/1000 dilution

Lane 1:

PC-12 whole cell lysates at 30 µg

Lane 2:

Rat2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 48 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human RPL4 aa 50-350. The exact immunogen used to generate this antibody is proprietary information.

P36578

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPL4 also known as 60S ribosomal protein L4 is a protein that plays an essential role in the ribosome the molecular machine responsible for protein synthesis. The protein has a mass of approximately 47 kDa. RPL4 contributes to ribosomal structure and function participating in the assembly of the large 60S subunit of the ribosome. This protein is ubiquitously expressed in human tissues indicating a broad involvement in cellular processes requiring protein production across different organs.

Biological function summary

RPL4 functions as a component of the ribosomal complex. By forming part of the 60S subunit it assists in translating mRNA into polypeptides during protein synthesis. This protein also interacts with ribosomal RNA (rRNA) and other ribosomal proteins to maintain the structural integrity of the ribosome. As a member of the larger ribosomal machinery RPL4 helps coordinate the interplay between translation and cellular growth ensuring that cells produce proteins at the necessary rate for various physiological needs.

Pathways

RPL4 plays a significant role in the protein synthesis pathway. It links with other ribosomal proteins such as RPL5 and RPS6 within the translation machinery. This relationship integrates RPL4 into the mRNA translation pathway important for gene expression regulation. Additionally RPL4 participates in the ribosome biogenesis process where it collaborates with proteins like NPM1 to generate mature ribosomal subunits. Through these interactions RPL4 supports both the synthesis and assembly of ribosomes impacting cell growth and proliferation pathways.

Abnormalities in RPL4 are associated with certain cancers including gastric cancer. Mutations or dysregulation of RPL4 may influence tumor progression by altering protein synthesis rates. The protein also relates to Diamond-Blackfan anemia (DBA) a rare blood disorder characterized by faulty ribosome function. In DBA RPL4 dysfunction can lead to reduced red blood cell production connecting it to other ribosomal proteins like RPS19 also implicated in the disorder. Both diseases underline the importance of RPL4 in maintaining healthy cellular and physiological functions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.

See full target information RPL4

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in physiology 8:281 PubMed28533755

2017

Proteomic Analysis of Chicken Skeletal Muscle during Embryonic Development.

Applications

Species

Unspecified reactive species

Hongjia Ouyang,Zhijun Wang,Xiaolan Chen,Jiao Yu,Zhenhui Li,Qinghua Nie

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPL4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL4 antibody (AB154907)

Immunocytochemistry/ Immunofluorescence - Anti-RPL4 antibody (AB154907)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL4 antibody (AB154907)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL4 antibody (AB154907)

Western blot - Anti-RPL4 antibody (AB154907)

Western blot - Anti-RPL4 antibody (AB154907)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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