Rabbit Polyclonal RPL5 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RPL5 aa 100 to C-terminus.
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- Cell reports 41:1115712022Nuclear stabilization of p53 requires a functional nucleolar surveillance pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatherine M Hannan,Priscilla Soo,Mei S Wong,Justine K Lee,Nadine Hein,Perlita Poh,Kira D Wysoke,Tobias D Williams,Christian Montellese,Lorey K Smith,Sheren J Al-Obaidi,Lorena Núñez-Villacís,Megan Pavy,Jin-Shu He,Kate M Parsons,Karagh E Loring,Tess Morrison,Jeannine Diesch,Gaetan Burgio,Rita Ferreira,Zhi-Ping Feng,Cathryn M Gould,Piyush B Madhamshettiwar,Johan Flygare,Thomas J Gonda,Kaylene J Simpson,Ulrike Kutay,Richard B Pearson,Christoph Engel,Nicholas J Watkins,Ross D Hannan,Amee J GeorgePubMed 36323262
- FEBS letters 596:2834-28502022ISGylation directly modifies hypoxia-inducible factor-2α and enhances its polysome association.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGaelan Melanson,Antonia C Du Bois,Caroline Webster,James UniackePubMed 36053046
- Nucleic acid therapeutics 30:312-3242020Gapmer Antisense Oligonucleotides Targeting 5S Ribosomal RNA Can Reduce Mature 5S Ribosomal RNA by Two Mechanisms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdam J Pollak et. al.PubMed 32589504
- Molecular and cellular biology 40:2020DEAD Box Protein Family Member DDX28 Is a Negative Regulator of Hypoxia-Inducible Factor 2α- and Eukaryotic Initiation Factor 4E2-Directed Hypoxic Translation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSonia L Evagelou et. al.PubMed 31907278
- RNA (New York, N.Y.) 26:361-3712020Hypoxia influences polysome distribution of human ribosomal protein S12 and alternative splicing of ribosomal protein mRNAs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrea Brumwell et. al.PubMed 31911497
- The Journal of biological chemistry 291:10772-822016Human Cells Cultured under Physiological Oxygen Utilize Two Cap-binding Proteins to recruit Distinct mRNAs for Translation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSara Timpano et. al.PubMed 27002144
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human RPL5 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P46777
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Predicted | Predicted | Predicted |
| Chicken | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Rabbit | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Chicken, Cow, Pig | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Chicken, Cow, Pig | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Chicken, Cow, Pig | Dilution info - | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. As part of the 5S RNP/5S ribonucleoprotein particle it is an essential component of the LSU, required for its formation and the maturation of rRNAs (PubMed:12962325, PubMed:19061985, PubMed:23636399, PubMed:24120868). It also couples ribosome biogenesis to p53/TP53 activation. As part of the 5S RNP it accumulates in the nucleoplasm and inhibits MDM2, when ribosome biogenesis is perturbed, mediating the stabilization and the activation of TP53 (PubMed:24120868).
Alternative names
MSTP030, RPL5, Large ribosomal subunit protein uL18, 60S ribosomal protein L5
Recommended products
Rabbit Polyclonal RPL5 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RPL5 aa 100 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human RPL5 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P46777
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RPL5 also known as ribosomal protein L5 or uL18 is a component of the large 60S subunit of ribosomes. Its molecular weight is approximately 34 kDa. This protein plays an important role in protein synthesis by facilitating the assembly of the 5S ribonucleoprotein complex. RPL5 is expressed ubiquitously across various tissues indicating its role in essential cellular processes.
- Biological function summary
The actions of RPL5 include its involvement in the incorporation of the 5S rRNA into the ribosomal subunit in cooperation with other ribosomal proteins. RPL5 is part of the ribosomal complex which is fundamental for translating mRNA into polypeptides. By binding with 5S rRNA RPL5 ensures the structural integrity and functional competence of the ribosome which are important for gene expression and cellular homeostasis.
- Pathways
RPL5 plays a critical role in the ribosome biogenesis pathway and indirectly influences the p53 pathway. It interacts with RPL11 to regulate the p53 response during ribosomal stress which connects RPL5 to the ribosomal stress checkpoint pathway. Deviations in ribosomal assembly or function can influence processes linked to cell cycle regulation and apoptosis.
- Associated diseases and disorders
Alterations in RPL5 have a link to Diamond-Blackfan anemia a disorder characterized by failed ribosome biogenesis affecting erythropoiesis. Mutations in RPL5 in association with failures in the RPL11 gene contribute to this condition by disrupting the proper assembly of ribosomes leading to impaired red blood cell production. Additionally dysregulation of the p53 pathway through RPL5 interaction may have implications in cancer development as it can affect cell growth control and apoptosis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-RPL5 antibody (ab137617)
12% SDS PAGE
All lanes: Western blot - Anti-RPL5 antibody (ab137617) at 1/1000 dilution
Lane 1: A431 whole cell lysate at 30 µg
Lane 2: H1299 whole cell lysate at 30 µg
Lane 3: HeLaS3 whole cell lysate at 30 µg
Lane 4: MOLT4 whole cell lysate at 30 µg
Predicted band size: 34 kDa
Western blot - Anti-RPL5 antibody (ab137617)
10% SDS PAGE
All lanes: Western blot - Anti-RPL5 antibody (ab137617) at 1/1000 dilution
All lanes: Mouse brain whole cell lysate at 50 µg
Predicted band size: 34 kDa
Immunocytochemistry/ Immunofluorescence - Anti-RPL5 antibody (ab137617)
ab137617 at 1/500 dilution staining RPL5 in 4% paraformaldehyde (room temperature for 15 min) fixed HepG2 cells by Immunofluorescence (green). Blue staining: Hoechst 33343.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL5 antibody (ab137617)
ab137617 at 1/100 dilution, staining RPL5 in paraffin embedded Gastric CA N87 xenograft tissue by Immunohistochemistry.
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