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AB137617

Anti-RPL5 antibody

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(7 Publications)

Rabbit Polyclonal RPL5 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RPL5 aa 100 to C-terminus.

View Alternative Names

MSTP030, RPL5, Large ribosomal subunit protein uL18, 60S ribosomal protein L5

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-RPL5 antibody (AB137617)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RPL5 antibody (AB137617)

Immunofluorescence analysis of HepG2 cells fixed in 4% paraformaldehyde at RT for 15 min labelling RPL5 protein at cytoplasm using ab137617 at a 1/500 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue). Scale bar = 10 µm

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL5 antibody (AB137617)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL5 antibody (AB137617)

Immunohistochemical analysis of paraffin embedded mouse hind brain tissue stained for RPL5 protein at cytoplasm with ab137617 at a 1/500 dilution.

Antigen retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Western blot - Anti-RPL5 antibody (AB137617)
  • WB

Unknown

Western blot - Anti-RPL5 antibody (AB137617)

10% SDS PAGE

All lanes:

Western blot - Anti-RPL5 antibody (ab137617) at 1/1000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 34 kDa

false

Western blot - Anti-RPL5 antibody (AB137617)
  • WB

Supplier Data

Western blot - Anti-RPL5 antibody (AB137617)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-RPL5 antibody (ab137617) at 1/1000 dilution

All lanes:

Rat2 whole cell lysate at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 34 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human RPL5 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P46777

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPL5 also known as ribosomal protein L5 or uL18 is a component of the large 60S subunit of ribosomes. Its molecular weight is approximately 34 kDa. This protein plays an important role in protein synthesis by facilitating the assembly of the 5S ribonucleoprotein complex. RPL5 is expressed ubiquitously across various tissues indicating its role in essential cellular processes.
Biological function summary

The actions of RPL5 include its involvement in the incorporation of the 5S rRNA into the ribosomal subunit in cooperation with other ribosomal proteins. RPL5 is part of the ribosomal complex which is fundamental for translating mRNA into polypeptides. By binding with 5S rRNA RPL5 ensures the structural integrity and functional competence of the ribosome which are important for gene expression and cellular homeostasis.

Pathways

RPL5 plays a critical role in the ribosome biogenesis pathway and indirectly influences the p53 pathway. It interacts with RPL11 to regulate the p53 response during ribosomal stress which connects RPL5 to the ribosomal stress checkpoint pathway. Deviations in ribosomal assembly or function can influence processes linked to cell cycle regulation and apoptosis.

Alterations in RPL5 have a link to Diamond-Blackfan anemia a disorder characterized by failed ribosome biogenesis affecting erythropoiesis. Mutations in RPL5 in association with failures in the RPL11 gene contribute to this condition by disrupting the proper assembly of ribosomes leading to impaired red blood cell production. Additionally dysregulation of the p53 pathway through RPL5 interaction may have implications in cancer development as it can affect cell growth control and apoptosis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. As part of the 5S RNP/5S ribonucleoprotein particle it is an essential component of the LSU, required for its formation and the maturation of rRNAs (PubMed : 12962325, PubMed : 19061985, PubMed : 23636399, PubMed : 24120868). It also couples ribosome biogenesis to p53/TP53 activation. As part of the 5S RNP it accumulates in the nucleoplasm and inhibits MDM2, when ribosome biogenesis is perturbed, mediating the stabilization and the activation of TP53 (PubMed : 24120868).
See full target information RPL5
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

eLife 12: PubMed36989136

2023

Integrated transcriptome and proteome analysis reveals posttranscriptional regulation of ribosomal genes in human brain organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Jaydeep Sidhaye,Philipp Trepte,Natalie Sepke,Maria Novatchkova,Michael Schutzbier,Gerhard Dürnberger,Karl Mechtler,Jürgen A Knoblich

Cell reports 41:111571 PubMed36323262

2022

Nuclear stabilization of p53 requires a functional nucleolar surveillance pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine M Hannan,Priscilla Soo,Mei S Wong,Justine K Lee,Nadine Hein,Perlita Poh,Kira D Wysoke,Tobias D Williams,Christian Montellese,Lorey K Smith,Sheren J Al-Obaidi,Lorena Núñez-Villacís,Megan Pavy,Jin-Shu He,Kate M Parsons,Karagh E Loring,Tess Morrison,Jeannine Diesch,Gaetan Burgio,Rita Ferreira,Zhi-Ping Feng,Cathryn M Gould,Piyush B Madhamshettiwar,Johan Flygare,Thomas J Gonda,Kaylene J Simpson,Ulrike Kutay,Richard B Pearson,Christoph Engel,Nicholas J Watkins,Ross D Hannan,Amee J George

FEBS letters 596:2834-2850 PubMed36053046

2022

ISGylation directly modifies hypoxia-inducible factor-2α and enhances its polysome association.

Applications

Unspecified application

Species

Unspecified reactive species

Gaelan Melanson,Antonia C Du Bois,Caroline Webster,James Uniacke

Nucleic acid therapeutics 30:312-324 PubMed32589504

2020

Gapmer Antisense Oligonucleotides Targeting 5S Ribosomal RNA Can Reduce Mature 5S Ribosomal RNA by Two Mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Adam J Pollak,Justin H Hickman,Xue-Hai Liang,Stanley T Crooke

Molecular and cellular biology 40: PubMed31907278

2020

DEAD Box Protein Family Member DDX28 Is a Negative Regulator of Hypoxia-Inducible Factor 2α- and Eukaryotic Initiation Factor 4E2-Directed Hypoxic Translation.

Applications

Unspecified application

Species

Unspecified reactive species

Sonia L Evagelou,Olivia Bebenek,Erin J Specker,James Uniacke

RNA (New York, N.Y.) 26:361-371 PubMed31911497

2020

Hypoxia influences polysome distribution of human ribosomal protein S12 and alternative splicing of ribosomal protein mRNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Brumwell,Leslie Fell,Lindsay Obress,James Uniacke

The Journal of biological chemistry 291:10772-82 PubMed27002144

2016

Human Cells Cultured under Physiological Oxygen Utilize Two Cap-binding Proteins to recruit Distinct mRNAs for Translation.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Timpano,James Uniacke
View all publications
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