AB157099

Anti-RPL5 antibody

Name: Anti-RPL5 antibody (ab157099) | Abcam
Brand: Abcam Limited
SKU: AB157099
Price: 535 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal RPL5 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human RPL5 aa 200 to C-terminus.

View Alternative Names

MSTP030, RPL5, Large ribosomal subunit protein uL18, 60S ribosomal protein L5

2 Images

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Immunoprecipitation - Anti-RPL5 antibody (AB157099)

Detection of RPL5 by Western Blot of Immunprecipitate.
Lane 1 : ab157099 at 1µg/ml labelling RPL5 in 293T whole cell lysate immunoprecipitated using ab157099 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2 : Control IgG
Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-RPL5 antibody (ab157099)

Predicted band size: 34 kDa

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Supplier Data

Western blot - Anti-RPL5 antibody (AB157099)

All lanes:

Western blot - Anti-RPL5 antibody (ab157099) at 0.1 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Lane 5:

3T3 whole cell lysate at 50 µg

Predicted band size: 34 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human RPL5 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P46777

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPL5 also known as ribosomal protein L5 or uL18 is a component of the large 60S subunit of ribosomes. Its molecular weight is approximately 34 kDa. This protein plays an important role in protein synthesis by facilitating the assembly of the 5S ribonucleoprotein complex. RPL5 is expressed ubiquitously across various tissues indicating its role in essential cellular processes.

Biological function summary

The actions of RPL5 include its involvement in the incorporation of the 5S rRNA into the ribosomal subunit in cooperation with other ribosomal proteins. RPL5 is part of the ribosomal complex which is fundamental for translating mRNA into polypeptides. By binding with 5S rRNA RPL5 ensures the structural integrity and functional competence of the ribosome which are important for gene expression and cellular homeostasis.

Pathways

RPL5 plays a critical role in the ribosome biogenesis pathway and indirectly influences the p53 pathway. It interacts with RPL11 to regulate the p53 response during ribosomal stress which connects RPL5 to the ribosomal stress checkpoint pathway. Deviations in ribosomal assembly or function can influence processes linked to cell cycle regulation and apoptosis.

Alterations in RPL5 have a link to Diamond-Blackfan anemia a disorder characterized by failed ribosome biogenesis affecting erythropoiesis. Mutations in RPL5 in association with failures in the RPL11 gene contribute to this condition by disrupting the proper assembly of ribosomes leading to impaired red blood cell production. Additionally dysregulation of the p53 pathway through RPL5 interaction may have implications in cancer development as it can affect cell growth control and apoptosis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. As part of the 5S RNP/5S ribonucleoprotein particle it is an essential component of the LSU, required for its formation and the maturation of rRNAs (PubMed : 12962325, PubMed : 19061985, PubMed : 23636399, PubMed : 24120868). It also couples ribosome biogenesis to p53/TP53 activation. As part of the 5S RNP it accumulates in the nucleoplasm and inhibits MDM2, when ribosome biogenesis is perturbed, mediating the stabilization and the activation of TP53 (PubMed : 24120868).

See full target information RPL5

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Science (New York, N.Y.) 386:eadq8587 PubMed39571015

2024

Specific tRNAs promote mRNA decay by recruiting the CCR4-NOT complex to translating ribosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqiang Zhu,Victor Emmanuel Cruz,He Zhang,Jan P Erzberger,Joshua T Mendell

Cell reports 42:112028 PubMed36848233

2023

SAYSD1 senses UFMylated ribosome to safeguard co-translational protein translocation at the endoplasmic reticulum.

Applications

Unspecified application

Species

Unspecified reactive species

Lihui Wang,Yue Xu,Sijung Yun,Quan Yuan,Prasanna Satpute-Krishnan,Yihong Ye

Oncogene 41:1155-1165 PubMed34999733

2022

Puf-A promotes cancer progression by interacting with nucleophosmin in nucleolus.

Applications

Unspecified application

Species

Unspecified reactive species

Huan-Chieh Cho,Yenlin Huang,Jung-Tung Hung,Tsai-Hsien Hung,Kai-Chun Cheng,Yun-Hen Liu,Ming-Wei Kuo,Sheng-Hung Wang,Alice L Yu,John Yu

Cell death and differentiation 29:687-696 PubMed34611297

2021

RNA-interference screen for p53 regulators unveils a role of WDR75 in ribosome biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Pavel Moudry,Katarina Chroma,Sladana Bursac,Sinisa Volarevic,Jiri Bartek

Nucleic acids research 48:862-878 PubMed31740951

2019

The FMRP-MOV10 complex: a translational regulatory switch modulated by G-Quadruplexes.

Applications

Unspecified application

Species

Unspecified reactive species

Phillip J Kenny,Miri Kim,Geena Skariah,Joshua Nielsen,Monica C Lannom,Stephanie Ceman

Cell cycle (Georgetown, Tex.) 17:92-101 PubMed29143558

2017

Perturbation of RNA Polymerase I transcription machinery by ablation of HEATR1 triggers the RPL5/RPL11-MDM2-p53 ribosome biogenesis stress checkpoint pathway in human cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zsofia Turi,Marketa Senkyrikova,Martin Mistrik,Jiri Bartek,Pavel Moudry

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-RPL5 antibody

Immunoprecipitation - Anti-RPL5 antibody (AB157099)

Western blot - Anti-RPL5 antibody (AB157099)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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