Rabbit Polyclonal RPL5 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human RPL5 aa 200 to C-terminus.
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Publications
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- Cell reports 42:1120282023SAYSD1 senses UFMylated ribosome to safeguard co-translational protein translocation at the endoplasmic reticulum.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLihui Wang,Yue Xu,Sijung Yun,Quan Yuan,Prasanna Satpute-Krishnan,Yihong YePubMed 36848233
- Oncogene 41:1155-11652022Puf-A promotes cancer progression by interacting with nucleophosmin in nucleolus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuan-Chieh Cho,Yenlin Huang,Jung-Tung Hung,Tsai-Hsien Hung,Kai-Chun Cheng,Yun-Hen Liu,Ming-Wei Kuo,Sheng-Hung Wang,Alice L Yu,John YuPubMed 34999733
- Cell death and differentiation 29:687-6962021RNA-interference screen for p53 regulators unveils a role of WDR75 in ribosome biogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPavel Moudry,Katarina Chroma,Sladana Bursac,Sinisa Volarevic,Jiri BartekPubMed 34611297
- Nucleic acids research 48:862-8782019The FMRP-MOV10 complex: a translational regulatory switch modulated by G-Quadruplexes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPhillip J Kenny,Miri Kim,Geena Skariah,Joshua Nielsen,Monica C Lannom,Stephanie CemanPubMed 31740951
- Cell cycle (Georgetown, Tex.) 17:92-1012017Perturbation of RNA Polymerase I transcription machinery by ablation of HEATR1 triggers the RPL5/RPL11-MDM2-p53 ribosome biogenesis stress checkpoint pathway in human cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZsofia Turi,Marketa Senkyrikova,Martin Mistrik,Jiri Bartek,Pavel MoudryPubMed 29143558
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human RPL5 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P46777
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Chinese hamster | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Cynomolgus monkey | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Elephant | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Guinea pig | Predicted | Predicted |
| Horse | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Horse, Guinea pig, Cow, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Chinese hamster, Elephant, Dog, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species Mouse | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Horse, Guinea pig, Cow, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Chinese hamster, Elephant, Dog, Orangutan | Dilution info - | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. As part of the 5S RNP/5S ribonucleoprotein particle it is an essential component of the LSU, required for its formation and the maturation of rRNAs (PubMed:12962325, PubMed:19061985, PubMed:23636399, PubMed:24120868). It also couples ribosome biogenesis to p53/TP53 activation. As part of the 5S RNP it accumulates in the nucleoplasm and inhibits MDM2, when ribosome biogenesis is perturbed, mediating the stabilization and the activation of TP53 (PubMed:24120868).
Alternative names
MSTP030, RPL5, Large ribosomal subunit protein uL18, 60S ribosomal protein L5
Recommended products
Rabbit Polyclonal RPL5 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human RPL5 aa 200 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human RPL5 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P46777
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RPL5 also known as ribosomal protein L5 or uL18 is a component of the large 60S subunit of ribosomes. Its molecular weight is approximately 34 kDa. This protein plays an important role in protein synthesis by facilitating the assembly of the 5S ribonucleoprotein complex. RPL5 is expressed ubiquitously across various tissues indicating its role in essential cellular processes.
- Biological function summary
The actions of RPL5 include its involvement in the incorporation of the 5S rRNA into the ribosomal subunit in cooperation with other ribosomal proteins. RPL5 is part of the ribosomal complex which is fundamental for translating mRNA into polypeptides. By binding with 5S rRNA RPL5 ensures the structural integrity and functional competence of the ribosome which are important for gene expression and cellular homeostasis.
- Pathways
RPL5 plays a critical role in the ribosome biogenesis pathway and indirectly influences the p53 pathway. It interacts with RPL11 to regulate the p53 response during ribosomal stress which connects RPL5 to the ribosomal stress checkpoint pathway. Deviations in ribosomal assembly or function can influence processes linked to cell cycle regulation and apoptosis.
- Associated diseases and disorders
Alterations in RPL5 have a link to Diamond-Blackfan anemia a disorder characterized by failed ribosome biogenesis affecting erythropoiesis. Mutations in RPL5 in association with failures in the RPL11 gene contribute to this condition by disrupting the proper assembly of ribosomes leading to impaired red blood cell production. Additionally dysregulation of the p53 pathway through RPL5 interaction may have implications in cancer development as it can affect cell growth control and apoptosis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-RPL5 antibody (ab157099)
All lanes: Western blot - Anti-RPL5 antibody (ab157099) at 0.1 µg/mL
Lane 1: 293T whole cell lysate at 50 µg
Lane 2: 293T whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 50 µg
Lane 4: Jurkat whole cell lysate at 50 µg
Lane 5: 3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 34 kDa
Exposure time: 10s
Immunoprecipitation - Anti-RPL5 antibody (ab157099)
Detection of RPL5 by Western Blot of Immunprecipitate.
Lane 1: ab157099 at 1µg/ml labelling RPL5 in 293T whole cell lysate immunoprecipitated using ab157099 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 10 seconds.All lanes: Immunoprecipitation - Anti-RPL5 antibody (ab157099)
Predicted band size: 34 kDa
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