JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB72550

Anti-RPL7 antibody

5

(1 Review)

|

(32 Publications)

Rabbit Polyclonal RPL7 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 32 publications. Immunogen corresponding to Synthetic Peptide within Human RPL7.

View Alternative Names

Large ribosomal subunit protein uL30, 60S ribosomal protein L7, RPL7

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7 antibody (AB72550)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7 antibody (AB72550)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma and bronchioalveolar carcinoma pleural effusion tissue labelling RPL7 with ab72550 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection : DAB.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7 antibody (AB72550)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7 antibody (AB72550)

IHC image of ab72550 staining in human normal cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72550, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Immunoprecipitation - Anti-RPL7 antibody (AB72550)
  • IP

Unknown

Immunoprecipitation - Anti-RPL7 antibody (AB72550)

Detection of Human RPL7 by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72550 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.

All lanes:

Immunoprecipitation - Anti-RPL7 antibody (ab72550)

Predicted band size: 29 kDa

false

Western blot - Anti-RPL7 antibody (AB72550)
  • WB

AbReview48943****

Western blot - Anti-RPL7 antibody (AB72550)

Blocked with 2% milk for 1 hour at 18°C.

Incubated with the primary antibody in 2% milk for 18 hours at 4°C.

All lanes:

Western blot - Anti-RPL7 antibody (ab72550)

Lane 1:

U2OS whole cell lysate treated with control siRNA at 10 µg

Lane 2:

U2OS whole cell lysate treated with RPL7 siRNA at 10 µg

Secondary

All lanes:

HRP-conjugated donkey anti-rabbit IgG polyclonal at 1/1 dilution

Predicted band size: 29 kDa

Observed band size: 30 kDa

true

Exposure time: 10s

This image is courtesy of an Abreview submitted by Loren Gibson

Western blot - Anti-RPL7 antibody (AB72550)
  • WB

Unknown

Western blot - Anti-RPL7 antibody (AB72550)

All lanes:

Western blot - Anti-RPL7 antibody (ab72550) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, IP, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human RPL7. The exact immunogen used to generate this antibody is proprietary information.

P18124

style
left-column

Complementary Products

Primary Antibodies

AB172478

Anti-RPL7A antibody [EPR12310]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody [EPR12310] (AB172478)

  • 0
  • 0
Primary Antibodies

AB169538

Anti-RPL8 antibody [EPR11630]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-RPL8 antibody [EPR11630] (AB169538)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB75748

Anti-EEF2/Elongation factor 2 antibody [EP880Y]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2/Elongation factor 2 antibody [EP880Y] (AB75748)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 mg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Catfish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Turkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPL7 also known as Ribosomal Protein L7 is an important component of the 60S large ribosomal subunit and plays a fundamental role in protein synthesis. This protein weighs approximately 29 kDa and is involved in the formation and assembly of the ribosome. RPL7 is expressed in a wide range of tissues indicating its ubiquitous role in cellular processes. It interacts with RNA contributing to the stabilization and proper function of ribosomal units.
Biological function summary

RPL7 engages in ribosomal structure and function participating in the translation process. It is a part of the large ribosomal complex the 60S subunit which works in concert with 40S subunit during protein synthesis. RPL7 binds RNA and plays a role in aligning mRNA and tRNAs in the ribosome facilitating accurate translation and peptide bond formation. Additionally it contributes to the proper assembly of ribosomal proteins and RNA components.

Pathways

RPL7 plays a role in the translational control pathway and ribosome biogenesis pathway. It interacts with other ribosomal proteins such as RPL13 and RPL10A and is essential for the proper progression of these pathways which impact protein production necessary for cell growth and differentiation. RPL7's involvement in these pathways emphasizes its contribution to cellular protein balance and homeostasis.

Alterations in RPL7 are associated with cancer and ribosomopathies. Changes in RPL7 expression levels can disrupt normal ribosomal function leading to uncontrolled cell proliferation seen in various cancers with potential connections to tumor suppressor proteins like p53. Also ribosomopathies involving ribosomal defects link RPL7 to conditions such as Diamond-Blackfan anemia a rare genetic disorder affecting blood cell production. RPL7's function and interactions highlight its relevance in maintaining cellular and organismal health.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Component of the large ribosomal subunit (PubMed : 12962325, PubMed : 23636399, PubMed : 32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 12962325, PubMed : 23636399, PubMed : 32669547). Binds to G-rich structures in 28S rRNA and in mRNAs (PubMed : 12962325). Plays a regulatory role in the translation apparatus; inhibits cell-free translation of mRNAs (PubMed : 12962325).
See full target information RPL7
style
left-column

Publications (32)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 44:801-823 PubMed39690241

2024

A nuclear protein quality control system for elimination of nucleolus-related inclusions.

Applications

Unspecified application

Species

Unspecified reactive species

Lorène Brunello,Jolanta Polanowska,Léo Le Tareau,Chantal Maghames,Virginie Georget,Charlotte Guette,Karima Chaoui,Stéphanie Balor,Marie-Françoise O'Donohue,Marie-Pierre Bousquet,Pierre-Emmanuel Gleizes,Dimitris P Xirodimas

Nucleic acids research 52:12021-12038 PubMed39217468

2024

ADAR3 modulates neuronal differentiation and regulates mRNA stability and translation.

Applications

Unspecified application

Species

Unspecified reactive species

Victor Karlström,Eduardo A Sagredo,Jordi Planells,Charlotte Welinder,Jennifer Jungfleisch,Andrea Barrera-Conde,Linus Engfors,Chammiran Daniel,Fátima Gebauer,Neus Visa,Marie Öhman

eLife 12: PubMed38512721

2024

Paradoxical imbalance between activated lymphocyte protein synthesis capacity and rapid division rate.

Applications

Unspecified application

Species

Unspecified reactive species

Mina O Seedhom,Devin Dersh,Jaroslav Holly,Mariana Pavon-Eternod,Jiajie Wei,Matthew Angel,Lucas Shores,Alexandre David,Jefferson Santos,Heather Hickman,Jonathan W Yewdell

Experimental neurology 368:114510 PubMed37633482

2023

Experimental upregulation of developmentally downregulated ribosomal protein large subunits 7 and 7A promotes axon regeneration after injury in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Xing,William C Theune,Agnieszka Lukomska,Matthew P Frost,Ashiti Damania,Ephraim F Trakhtenberg

Nucleic acids research 51:9983-10000 PubMed37602404

2023

Increased levels of eIF2A inhibit translation by sequestering 40S ribosomal subunits.

Applications

Unspecified application

Species

Unspecified reactive species

Daisy J Grove,Daniel J Levine,Michael G Kearse

Nucleic acids research 51:9397-9414 PubMed37526268

2023

The induction of p53 correlates with defects in the production, but not the levels, of the small ribosomal subunit and stalled large ribosomal subunit biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew John Eastham,Andria Pelava,Graeme Raymond Wells,Justine Katherine Lee,Isabella Rachel Lawrence,Joshua Stewart,Maria Deichner,Regina Hertle,Nicholas James Watkins,Claudia Schneider

Biomolecules 13: PubMed37371478

2023

RPS27a and RPL40, Which Are Produced as Ubiquitin Fusion Proteins, Are Not Essential for p53 Signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew John Eastham,Andria Pelava,Graeme Raymond Wells,Nicholas James Watkins,Claudia Schneider

The EMBO journal 42:e112362 PubMed37155573

2023

eIF3 mRNA selectivity profiling reveals eIF3k as a cancer-relevant regulator of ribosome content.

Applications

Unspecified application

Species

Unspecified reactive species

Haoran Duan,Siqiong Zhang,Yoram Zarai,Rupert Öllinger,Yanmeng Wu,Li Sun,Cheng Hu,Yaohui He,Guiyou Tian,Roland Rad,Xiangquan Kong,Yabin Cheng,Tamir Tuller,Dieter A Wolf

Nucleic acids research 51:5301-5324 PubMed36882085

2023

Dynamic interplay between RPL3- and RPL3L-containing ribosomes modulates mitochondrial activity in the mammalian heart.

Applications

Unspecified application

Species

Unspecified reactive species

Ivan Milenkovic,Helaine Graziele Santos Vieira,Morghan C Lucas,Jorge Ruiz-Orera,Giannino Patone,Scott Kesteven,Jianxin Wu,Michael Feneley,Guadalupe Espadas,Eduard Sabidó,Norbert Hübner,Sebastiaan van Heesch,Mirko Völkers,Eva Maria Novoa

Nature communications 13:2727 PubMed35585045

2022

HDLBP binds ER-targeted mRNAs by multivalent interactions to promote protein synthesis of transmembrane and secreted proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Ulrike Zinnall,Miha Milek,Igor Minia,Carlos H Vieira-Vieira,Simon Müller,Guido Mastrobuoni,Orsalia-Georgia Hazapis,Simone Del Giudice,David Schwefel,Nadine Bley,Franka Voigt,Jeffrey A Chao,Stefan Kempa,Stefan Hüttelmaier,Matthias Selbach,Markus Landthaler
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com