JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB155136

Anti-RPL8 antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal RPL8 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RPL8 aa 1-250.

View Alternative Names

Large ribosomal subunit protein uL2, 60S ribosomal protein L8, RPL8

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL8 antibody (AB155136)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL8 antibody (AB155136)

Immunohistochemical analysis of paraffin embedded NCIN87 xenograft labeling RPL8 with ab155136 antibody at 1/100.

Immunocytochemistry/ Immunofluorescence - Anti-RPL8 antibody (AB155136)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RPL8 antibody (AB155136)

Immunofluorescent analysis of methanol-fixed A431 cells labeling RPL8 with ab155136 at 1/200. Image at the bottom costained with Hoechst 33342.

Western blot - Anti-RPL8 antibody (AB155136)
  • WB

Unknown

Western blot - Anti-RPL8 antibody (AB155136)

12% SDS PAGE

All lanes:

Western blot - Anti-RPL8 antibody (ab155136) at 1/1000 dilution

Lane 1:

HepG2 whole cell lysate at 30 µg

Lane 2:

Molt4 whole cell lysate at 30 µg

Predicted band size: 28 kDa

false

Western blot - Anti-RPL8 antibody (AB155136)
  • WB

Unknown

Western blot - Anti-RPL8 antibody (AB155136)

12% SDS PAGE

All lanes:

Western blot - Anti-RPL8 antibody (ab155136) at 1/1000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human RPL8 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P62917

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPL8 also known as Ribosomal Protein L8 is a component of the 60S subunit of the ribosome playing a critical role in protein synthesis. It has a mass of approximately 26.1 kDa. RPL8 is expressed in a wide range of tissues including the liver kidney and brain reflecting its essential role in cellular function. This protein contributes directly to the structure and function of the ribosome facilitating the translation of mRNA into polypeptides.
Biological function summary

RPL8 serves as an integral part of the ribosomal machinery ensuring effective protein synthesis within cells. It is part of the large ribosomal subunit complex working alongside other ribosomal proteins to form a functional ribosome. This role is paramount for maintaining cellular homeostasis and supporting growth and proliferation by enabling balanced protein production.

Pathways

RPL8 is key within several fundamental biological pathways including the translation pathway and ribosome biogenesis. In ribosome biogenesis it interacts with proteins such as RPL5 and RPL11 contributing to the assembly and maturation of ribosomal subunits. This interaction facilitates the complex process of protein synthesis ensuring the correct translation of genetic information into functional proteins.

Abnormalities in RPL8 expression or function have been linked to certain cancers and Diamond-Blackfan anemia. In cancer dysfunction in ribosomal protein balance including RPL8 can lead to unchecked cellular proliferation. In Diamond-Blackfan anemia defects associated with RPL8 and other ribosomal proteins like RPS19 cause ineffective erythropoiesis leading to the anemia typically observed in this condition. Understanding these connections helps guide potential therapeutic interventions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.
See full target information RPL8
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 134: PubMed38875287

2024

Liver cancer initiation requires translational activation by an oncofetal regulon involving LIN28 proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Hsiung Hsieh,Yonglong Wei,Lin Li,Liem H Nguyen,Yu-Hsuan Lin,Jung M Yong,Xuxu Sun,Xun Wang,Xin Luo,Sarah K Knutson,Christina Bracken,George Q Daley,John T Powers,Hao Zhu

PloS one 14:e0210454 PubMed31034522

2019

miRNAs, target genes expression and morphological analysis on the heart in gestational protein-restricted offspring.

Applications

Unspecified application

Species

Unspecified reactive species

Heloisa Balan Assalin,José Antonio Rocha Gontijo,Patrícia Aline Boer
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB169538

Anti-RPL8 antibody [EPR11630]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-RPL8 antibody [EPR11630] (AB169538)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB196652

Anti-RPS24 antibody [EPR16017(B)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS24 antibody [EPR16017(B)] (AB196652)

  • 0
  • 0
Primary Antibodies

AB138125

Anti-RPL28 antibody

Western blot - Anti-RPL28 antibody (AB138125)

  • 4
  • 2
Primary Antibodies

AB72550

Anti-RPL7 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7 antibody (AB72550)

  • 5
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com