AB169538

Anti-RPL8 antibody [EPR11630]

RabMAb
Recombinant
What is this?

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(19 Publications)

Rabbit Recombinant Monoclonal RPL8 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 19 publications.

View Alternative Names

Large ribosomal subunit protein uL2, 60S ribosomal protein L8, RPL8

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-RPL8 antibody [EPR11630] (AB169538)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RPL8 antibody [EPR11630] (AB169538)

Immunofluorescent analysis of HepG2cells labeling RPL8 with ab169538 at 1/250.

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Immunoprecipitation - Anti-RPL8 antibody [EPR11630] (AB169538)

Immunoprecipitation. ab169538 at 1/10000 staining RPL8 in Jurkat cell lysate immunoprecipitated using ab169538 at 1/10. Predicted band size : 28 kDa.

All lanes:

Immunoprecipitation - Anti-RPL8 antibody [EPR11630] (ab169538)

Predicted band size: 28 kDa

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Western blot - Anti-RPL8 antibody [EPR11630] (AB169538)

All lanes:

Western blot - Anti-RPL8 antibody [EPR11630] (ab169538) at 1/10000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

SKBR-3 cell lysate at 10 µg

Lane 4:

Molt4 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Carrier free

Anti-RPL8 antibody [EPR11630] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11630

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPL8 also known as Ribosomal Protein L8 is a component of the 60S subunit of the ribosome playing a critical role in protein synthesis. It has a mass of approximately 26.1 kDa. RPL8 is expressed in a wide range of tissues including the liver kidney and brain reflecting its essential role in cellular function. This protein contributes directly to the structure and function of the ribosome facilitating the translation of mRNA into polypeptides.

Biological function summary

RPL8 serves as an integral part of the ribosomal machinery ensuring effective protein synthesis within cells. It is part of the large ribosomal subunit complex working alongside other ribosomal proteins to form a functional ribosome. This role is paramount for maintaining cellular homeostasis and supporting growth and proliferation by enabling balanced protein production.

Pathways

RPL8 is key within several fundamental biological pathways including the translation pathway and ribosome biogenesis. In ribosome biogenesis it interacts with proteins such as RPL5 and RPL11 contributing to the assembly and maturation of ribosomal subunits. This interaction facilitates the complex process of protein synthesis ensuring the correct translation of genetic information into functional proteins.

Abnormalities in RPL8 expression or function have been linked to certain cancers and Diamond-Blackfan anemia. In cancer dysfunction in ribosomal protein balance including RPL8 can lead to unchecked cellular proliferation. In Diamond-Blackfan anemia defects associated with RPL8 and other ribosomal proteins like RPS19 cause ineffective erythropoiesis leading to the anemia typically observed in this condition. Understanding these connections helps guide potential therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.

See full target information RPL8

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Molecular psychiatry 30:3599-3614 PubMed40089604

2025

Shank3 modulates Rpl3 expression and protein synthesis via mGlu5: implications for Phelan McDermid syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Federica Giona,Stefania Beretta,Antonio Zippo,Alessia Stefanoni,Zaira Tomasoni,Cinzia Vicidomini,Luisa Ponzoni,Mariaelvina Sala,Carrie K Jones,P Jeffrey Conn,Tobias M Boeckers,Carlo Sala,Chiara Verpelli

Molecular cell 85:815-828.e10 PubMed39879985

2025

Convergence of orphan quality control pathways at a ubiquitin chain-elongating ligase.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Carrillo Roas,Yuichi Yagita,Paul Murphy,Robert Kurzbauer,Tim Clausen,Eszter Zavodszky,Ramanujan S Hegde

Nature communications 15:9963 PubMed39551769

2024

Soluble αβ-tubulins reversibly sequester TTC5 to regulate tubulin mRNA decay.

Applications

Unspecified application

Species

Unspecified reactive species

Alina Batiuk,Markus Höpfler,Ana C Almeida,Deryn Teoh En-Jie,Oscar Vadas,Evangelia Vartholomaiou,Ramanujan S Hegde,Zhewang Lin,Ivana Gasic

Proceedings of the National Academy of Sciences of the United States of America 121:e2416722121 PubMed39436665

2024

AMBRA1 controls the translation of immune-specific genes in T lymphocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Simone Gottlieb,Wanjing Shang,Deji Ye,Satoshi Kubo,Ping Du Jiang,Samantha Shafer,Leilei Xu,Lixin Zheng,Ann Y Park,Jian Song,Waipan Chan,Zhiqin Zeng,Tingyan He,Benjamin Schwarz,Björn Häupl,Thomas Oellerich,Michael J Lenardo,Yikun Yao

Nature communications 15:6633 PubMed39117603

2024

Human tumor suppressor protein Pdcd4 binds at the mRNA entry channel in the 40S small ribosomal subunit.

Applications

Unspecified application

Species

Unspecified reactive species

Jailson Brito Querido,Masaaki Sokabe,Irene Díaz-López,Yuliya Gordiyenko,Philipp Zuber,Yifei Du,Lucas Albacete-Albacete,V Ramakrishnan,Christopher S Fraser

Experimental and therapeutic medicine 27:86 PubMed38274338

2024

HIF‑1 and macrophage activation signalling pathways are potential biomarkers of invasive aspergillosis.

Applications

Unspecified application

Species

Unspecified reactive species

Min Wang,Yuling Hu,Feng Cai,Jiayong Qiu,Yimin Mao,Yingmin Zhang

Nature chemical biology 20:877-884 PubMed38172604

2024

The eRF1 degrader SRI-41315 acts as a molecular glue at the ribosomal decoding center.

Applications

Unspecified application

Species

Unspecified reactive species

João P L Coelho,Matthew C J Yip,Keely Oltion,Jack Taunton,Sichen Shao

Cell 186:3443-3459.e24 PubMed37480851

2023

Mechanism of orphan subunit recognition during assembly quality control.

Applications

Unspecified application

Species

Unspecified reactive species

Yuichi Yagita,Eszter Zavodszky,Sew-Yeu Peak-Chew,Ramanujan S Hegde

Molecular cell 83:2290-2302.e13 PubMed37295431

2023

Mechanism of ribosome-associated mRNA degradation during tubulin autoregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Markus Höpfler,Eva Absmeier,Sew-Yeu Peak-Chew,Evangelia Vartholomaiou,Lori A Passmore,Ivana Gasic,Ramanujan S Hegde

Science advances 7:eabi5751 PubMed34890234

2021

The nuclear transcription factor, TAF7, is a cytoplasmic regulator of protein synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Cheng,Kevin Semmens,Elizabeth McManus,Qingrong Chen,Daoud Meerzaman,Xiantao Wang,Markus Hafner,Brian A Lewis,Hidehisa Takahashi,Ballachanda N Devaiah,Anne Gegonne,Dinah S Singer

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPL8 antibody [EPR11630]

Immunocytochemistry/ Immunofluorescence - Anti-RPL8 antibody [EPR11630] (AB169538)

Immunoprecipitation - Anti-RPL8 antibody [EPR11630] (AB169538)

Western blot - Anti-RPL8 antibody [EPR11630] (AB169538)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (19)

Product promise