AB102011

Anti-RPL9 antibody

Name: Anti-RPL9 antibody (ab102011) | Abcam
Brand: Abcam Limited
SKU: AB102011
Price: 545 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal RPL9 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human, Zebrafish samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RPL9 aa 1 to C-terminus.

View Alternative Names

OK/SW-cl.103, RPL9P7, RPL9P8, RPL9P9, RPL9, Large ribosomal subunit protein uL6, 60S ribosomal protein L9

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-RPL9 antibody (AB102011)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RPL9 antibody (AB102011)

Immunofluorescence analysis of methanol-fixed HeLa, using ab 102011 at 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL9 antibody (AB102011)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL9 antibody (AB102011)

Immunohistochemical analysis of paraffin embedded rt2 xenograft tissue with ab102011 at a 1/500 dilution.

Antigen retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL9 antibody (AB102011)

Immunohistochemical analysis of paraffin embedded mouse eye tissue stained for RPL9 protein at retinal pigment epithelium with ab102011 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Unknown

Western blot - Anti-RPL9 antibody (AB102011)

All lanes:

Western blot - Anti-RPL9 antibody (ab102011) at 1/1000 dilution

Lane 1:

HeLa whole cell lysate at 30 µg

Lane 2:

HepG2 whole cell lysate at 30 µg

Lane 3:

HCT116 whole cell lysate at 30 µg

Predicted band size: 22 kDa

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Unknown

Western blot - Anti-RPL9 antibody (AB102011)

All lanes:

Western blot - Anti-RPL9 antibody (ab102011) at 1/1000 dilution

All lanes:

mouse brain whole cell lysate at 50 µg

Predicted band size: 22 kDa

false

Supplier Data

Western blot - Anti-RPL9 antibody (AB102011)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-RPL9 antibody (ab102011) at 1/1000 dilution

All lanes:

Zebrafish muscle whole cell lysate at 30 µg

Predicted band size: 38 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Zebrafish

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human RPL9 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P32969

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The RPL9 protein also known as Ribosomal Protein L9 plays a role in the structure and function of ribosomes. This protein is part of the large ribosomal subunit facilitating the assembly of proteins. With a molecular mass of approximately 21 kDa RPL9 resides in the cytoplasm where ribosomes are largely found reflecting its function. Broadly expressed across various tissues it is essential for ribosomal integrity and efficient protein synthesis.

Biological function summary

RPL9 is involved in ribosome biogenesis and translation processes. As a component of the ribosomal complex it aids in the production of new proteins by translating messenger RNA (mRNA) into polypeptides. This function is tightly regulated and essential for maintaining cellular protein homeostasis. Additionally RPL9 participates in ribosomal assembly ensuring the proper folding and function of ribosomes.

Pathways

RPL9 plays an integral part in the mRNA translation pathway. It associates with other ribosomal proteins such as RPL5 and RPL11 forming a functional ribosomal unit. RPL9 contributes to the translation of mRNA into proteins an indispensable step in various cellular growth and proliferation pathways. Its interactions with these pathways highlight its role in regulating protein synthesis necessary for cell function.

RPL9 has been linked to Diamond-Blackfan Anemia (DBA) and other ribosomopathies. These conditions arise from defects in ribosomal protein production leading to impaired hematopoiesis. RPL9 mutations could affect the assembly of ribosomes similar to other ribosomal proteins like RPS19. These diseases underline the importance of RPL9 in maintaining ribosome function and cellular health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the large ribosomal subunit (PubMed : 23636399, PubMed : 32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399, PubMed : 32669547).

See full target information RPL9

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 48:770-787 PubMed31799629

2019

Ribosomal protein gene RPL9 variants can differentially impair ribosome function and cellular metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Marco Lezzerini,Marianna Penzo,Marie-Françoise O'Donohue,Carolina Marques Dos Santos Vieira,Manon Saby,Hyung L Elfrink,Illja J Diets,Anne-Marie Hesse,Yohann Couté,Marc Gastou,Alexandra Nin-Velez,Peter G J Nikkels,Alexandra N Olson,Evelien Zonneveld-Huijssoon,Marjolijn C J Jongmans,GuangJun Zhang,Michel van Weeghel,Riekelt H Houtkooper,Marcin W Wlodarski,Roland P Kuiper,Marc B Bierings,Jutte van der Werff Ten Bosch,Thierry Leblanc,Lorenzo Montanaro,Jonathan D Dinman,Lydie Da Costa,Pierre-Emmanuel Gleizes,Alyson W MacInnes

Journal of virology 87:1069-82 PubMed23135726

2012

Nucleolar trafficking of the mouse mammary tumor virus gag protein induced by interaction with ribosomal protein L9.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea R Beyer,Darrin V Bann,Breanna Rice,Ingrid S Pultz,Melissa Kane,Stephen P Goff,Tatyana V Golovkina,Leslie J Parent

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPL9 antibody

Immunocytochemistry/ Immunofluorescence - Anti-RPL9 antibody (AB102011)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL9 antibody (AB102011)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL9 antibody (AB102011)

Western blot - Anti-RPL9 antibody (AB102011)

Western blot - Anti-RPL9 antibody (AB102011)

Western blot - Anti-RPL9 antibody (AB102011)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise