Rabbit Polyclonal RPL9 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RPL9 aa 1 to C-terminus.
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Publications
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- Nucleic acids research 48:770-7872019Ribosomal protein gene RPL9 variants can differentially impair ribosome function and cellular metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarco Lezzerini,Marianna Penzo,Marie-Françoise O'Donohue,Carolina Marques Dos Santos Vieira,Manon Saby,Hyung L Elfrink,Illja J Diets,Anne-Marie Hesse,Yohann Couté,Marc Gastou,Alexandra Nin-Velez,Peter G J Nikkels,Alexandra N Olson,Evelien Zonneveld-Huijssoon,Marjolijn C J Jongmans,GuangJun Zhang,Michel van Weeghel,Riekelt H Houtkooper,Marcin W Wlodarski,Roland P Kuiper,Marc B Bierings,Jutte van der Werff Ten Bosch,Thierry Leblanc,Lorenzo Montanaro,Jonathan D Dinman,Lydie Da Costa,Pierre-Emmanuel Gleizes,Alyson W MacInnesPubMed 31799629
- Journal of virology 87:1069-822012Nucleolar trafficking of the mouse mammary tumor virus gag protein induced by interaction with ribosomal protein L9.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrea R Beyer,Darrin V Bann,Breanna Rice,Ingrid S Pultz,Melissa Kane,Stephen P Goff,Tatyana V Golovkina,Leslie J ParentPubMed 23135726
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human RPL9 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P32969
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Zebrafish | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/200.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Zebrafish | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Component of the large ribosomal subunit (PubMed:23636399, PubMed:32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:23636399, PubMed:32669547).
Alternative names
OK/SW-cl.103, RPL9P7, RPL9P8, RPL9P9, RPL9, Large ribosomal subunit protein uL6, 60S ribosomal protein L9
Recommended products
Rabbit Polyclonal RPL9 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RPL9 aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human RPL9 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P32969
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The RPL9 protein also known as Ribosomal Protein L9 plays a role in the structure and function of ribosomes. This protein is part of the large ribosomal subunit facilitating the assembly of proteins. With a molecular mass of approximately 21 kDa RPL9 resides in the cytoplasm where ribosomes are largely found reflecting its function. Broadly expressed across various tissues it is essential for ribosomal integrity and efficient protein synthesis.
- Biological function summary
RPL9 is involved in ribosome biogenesis and translation processes. As a component of the ribosomal complex it aids in the production of new proteins by translating messenger RNA (mRNA) into polypeptides. This function is tightly regulated and essential for maintaining cellular protein homeostasis. Additionally RPL9 participates in ribosomal assembly ensuring the proper folding and function of ribosomes.
- Pathways
RPL9 plays an integral part in the mRNA translation pathway. It associates with other ribosomal proteins such as RPL5 and RPL11 forming a functional ribosomal unit. RPL9 contributes to the translation of mRNA into proteins an indispensable step in various cellular growth and proliferation pathways. Its interactions with these pathways highlight its role in regulating protein synthesis necessary for cell function.
- Associated diseases and disorders
RPL9 has been linked to Diamond-Blackfan Anemia (DBA) and other ribosomopathies. These conditions arise from defects in ribosomal protein production leading to impaired hematopoiesis. RPL9 mutations could affect the assembly of ribosomes similar to other ribosomal proteins like RPS19. These diseases underline the importance of RPL9 in maintaining ribosome function and cellular health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunocytochemistry/ Immunofluorescence - Anti-RPL9 antibody (ab102011)
Immunofluorescence analysis of methanol-fixed HeLa, using ab 102011 at 1/200 dilution.
Western blot - Anti-RPL9 antibody (ab102011)
All lanes: Western blot - Anti-RPL9 antibody (ab102011) at 1/1000 dilution
Lane 1: HeLa whole cell lysate at 30 µg
Lane 2: HepG2 whole cell lysate at 30 µg
Lane 3: HCT116 whole cell lysate at 30 µg
Predicted band size: 22 kDa
Western blot - Anti-RPL9 antibody (ab102011)
All lanes: Western blot - Anti-RPL9 antibody (ab102011) at 1/1000 dilution
All lanes: mouse brain whole cell lysate at 50 µg
Predicted band size: 22 kDa
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