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AB154970

Anti-RPLP0 antibody

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(1 Publication)

Rabbit Polyclonal RPLP0 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human 60S acidic ribosomal protein P0 aa 50 to C-terminus.

View Alternative Names

Large ribosomal subunit protein uL10, 60S acidic ribosomal protein P0, 60S ribosomal protein L10E, RPLP0

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody (AB154970)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody (AB154970)

Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling RPLP0 with ab154970 at 1/500 dilution.

Western blot - Anti-RPLP0 antibody (AB154970)
  • WB

Unknown

Western blot - Anti-RPLP0 antibody (AB154970)

12% SDS PAGE

All lanes:

Western blot - Anti-RPLP0 antibody (ab154970) at 1/1000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Predicted band size: 34 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human 60S acidic ribosomal protein P0 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P05388

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPLP0 also known as 60S acidic ribosomal protein P0 is an integral component of the ribosomal machinery. It acts as a scaffold within the large 60S subunit of the ribosome which plays a fundamental role in protein synthesis. The protein has an approximate mass of 34.4 kDa. RPLP0 is ubiquitously expressed across various tissues indicating its essential role in cellular biology. Its expression is well-documented in highly proliferative tissues and organs reflecting the ribosome's central role in maintaining cellular growth and division.
Biological function summary

RPLP0 participates actively in ribosome structure and function. It forms part of the ribosomal stalk together with the P1 and P2 proteins facilitating interactions with GTPase translation factors during protein synthesis. This ribosomal complex is important for elongation phase of protein translation where it actively assists in the accurate and efficient elongation of polypeptide chains. By participating in the ribosome's structural framework RPLP0 drives the translation process influencing protein output in cells. This makes it essential for overall protein homeostasis.

Pathways

RPLP0 is deeply embedded within the processes of translation and ribosome biogenesis. It associates with the major pathway of ribosome assembly wherein new ribosomes are synthesized and configured. It functions alongside other ribosomal proteins like RPLP1 and RPLP2 which work together to maintain efficient protein synthesis. The interaction among these proteins within the translation pathway highlights the integrated nature of RPLP0 in facilitating cellular protein production.

RPLP0 has notable implications in cancer and Diamond-Blackfan anemia (DBA). Aberrations in ribosomal proteins including RPLP0 have been linked to oncogenesis where disrupted protein synthesis contributes to uncontrolled cellular proliferation. Additionally alterations in RPLP0 have associations with DBA a ribosomopathy characterized by defective red blood cell production. Within these disease contexts RPLP0's interaction with other ribosomal proteins like RPL11 can influence disease progression and cellular anomalies seen in such conditions.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Ribosomal protein P0 is the functional equivalent of E.coli protein L10.
See full target information 60S acidic ribosomal protein P0

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37046993

2023

Deciphering the Polyglucosan Accumulation Present in Lafora Disease Using an Astrocytic Cellular Model.

Applications

Unspecified application

Species

Unspecified reactive species

Mireia Moreno-Estellés,Ángela Campos-Rodríguez,Carla Rubio-Villena,Lorena Kumarasinghe,Maria Adelaida Garcia-Gimeno,Pascual Sanz
View all publications

Product promise

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