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Rabbit Monoclonal RPLP0 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 5 publications.


Images

Western blot - Anti-RPLP0 antibody [EP15646] (AB192866), expandable thumbnail
  • Western blot - Anti-RPLP0 antibody [EP15646] (AB192866), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] (AB192866), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] (AB192866), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] (AB192866), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-P
Human
Tested
Not recommended
Tested
Mouse
Tested
Not recommended
Tested
Rat
Tested
Not recommended
Tested

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Rat

Dilution info

-

Notes

-

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100 - 1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/100 - 1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/100 - 1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

2 products for Alternative Product

1 product for Alternative Version

Target data

Function

Ribosomal protein P0 is the functional equivalent of E.coli protein L10.

Alternative names

60S acidic ribosomal protein P0, 60S ribosomal protein L10E, Large ribosomal subunit protein uL10, RPLP0

Recommended products

Rabbit Monoclonal RPLP0 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 5 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EP15646

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

The production method for this product has been changed from hybridoma to recombinant on 18th June 2024.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

RPLP0 also known as 60S acidic ribosomal protein P0 is an integral component of the ribosomal machinery. It acts as a scaffold within the large 60S subunit of the ribosome which plays a fundamental role in protein synthesis. The protein has an approximate mass of 34.4 kDa. RPLP0 is ubiquitously expressed across various tissues indicating its essential role in cellular biology. Its expression is well-documented in highly proliferative tissues and organs reflecting the ribosome's central role in maintaining cellular growth and division.

Biological function summary

RPLP0 participates actively in ribosome structure and function. It forms part of the ribosomal stalk together with the P1 and P2 proteins facilitating interactions with GTPase translation factors during protein synthesis. This ribosomal complex is important for elongation phase of protein translation where it actively assists in the accurate and efficient elongation of polypeptide chains. By participating in the ribosome's structural framework RPLP0 drives the translation process influencing protein output in cells. This makes it essential for overall protein homeostasis.

Pathways

RPLP0 is deeply embedded within the processes of translation and ribosome biogenesis. It associates with the major pathway of ribosome assembly wherein new ribosomes are synthesized and configured. It functions alongside other ribosomal proteins like RPLP1 and RPLP2 which work together to maintain efficient protein synthesis. The interaction among these proteins within the translation pathway highlights the integrated nature of RPLP0 in facilitating cellular protein production.

Associated diseases and disorders

RPLP0 has notable implications in cancer and Diamond-Blackfan anemia (DBA). Aberrations in ribosomal proteins including RPLP0 have been linked to oncogenesis where disrupted protein synthesis contributes to uncontrolled cellular proliferation. Additionally alterations in RPLP0 have associations with DBA a ribosomopathy characterized by defective red blood cell production. Within these disease contexts RPLP0's interaction with other ribosomal proteins like RPL11 can influence disease progression and cellular anomalies seen in such conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

  • Western blot - Anti-RPLP0 antibody [EP15646] (ab192866), expandable thumbnail

    Western blot - Anti-RPLP0 antibody [EP15646] (ab192866)

    All lanes: Western blot - Anti-RPLP0 antibody [EP15646] (ab192866) at 1/20000 dilution

    Lane 1: 293 cell lysate at 20 µg

    Lane 2: HeLa cell lysate at 20 µg

    Lane 3: Jurkat cell lysate at 20 µg

    Lane 4: A375 cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa

    Observed band size: 34 kDa

  • Western blot - Anti-RPLP0 antibody [EP15646] (ab192866), expandable thumbnail

    Western blot - Anti-RPLP0 antibody [EP15646] (ab192866)

    All lanes: Western blot - Anti-RPLP0 antibody [EP15646] (ab192866) at 1/1000 dilution

    Lane 1: Mouse kidney lysate at 10 µg

    Lane 2: Mouse spleen lysate at 10 µg

    Lane 3: Rat brain lysate at 10 µg

    Lane 4: Rat spleen lysate at 10 µg

    Lane 5: C6 cell lysate at 10 µg

    Lane 6: RAW264.7 cell lysate at 10 µg

    Lane 7: PC12 cell lysate at 10 µg

    Lane 8: NIH 3T3 cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa

    Observed band size: 34 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] (ab192866), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] (ab192866)

    Immunohistochemical analysis of paraffin embedded Human stomach tissue sections labeling RPLP0 using ab192866 at a 1/100 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] (ab192866), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] (ab192866)

    Immunohistochemical analysis of paraffin embedded rmouse liver tissue sections labeling RPLP0 using ab192866 at a 1/100 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] (ab192866), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] (ab192866)

    Immunohistochemical analysis of paraffin embedded rat cerebral cortex tissue sections labeling RPLP0 using ab192866 at a 1/100 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-RPLP0 antibody [EP15646] (ab192866), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-RPLP0 antibody [EP15646] (ab192866)

    Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa cells labeling RPLP0 with ab192866 at a 1/1000 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary at a 1/400 dilution. Counterstain DAPI. Cells were permeabilized using 0.1% Triton X-100.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] (ab192866), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] (ab192866)

    Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling RPLP0 with ab192866 at 1/500 (2.312 ug/ml) dilution following a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on rat colon

    The sections were using heat mediated antigen retrieval with Tris-EDTA (pH 9.0, epitope retrieval solution 2) for 20 mins, then labeling ab192866 at 1/100 and 1/500 dilution for 30 mins at room temperature.
    Counterstained with hematoxylin.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] (ab192866), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] (ab192866)

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling RPLP0 with ab192866 at 1/500 (2.312 ug/ml) dilution following a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse colon

    The sections were using heat mediated antigen retrieval with Tris-EDTA (pH 9.0, epitope retrieval solution 2) for 20 mins, then labeling ab192866 at 1/100 and 1/500 dilution for 30 mins at room temperature.
    Counterstained with hematoxylin.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] (ab192866), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] (ab192866)

    Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling RPLP0 with ab192866 at 1/100 (11.56 ug/ml) dilution following a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human breast

    The sections were using heat mediated antigen retrieval with Tris-EDTA (pH 9.0, epitope retrieval solution 2) for 20 mins, then labeling ab192866 at 1/100 and 1/500 dilution for 30 mins at room temperature.
    Counterstained with hematoxylin.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Western blot - Anti-RPLP0 antibody [EP15646] (ab192866), expandable thumbnail

    Western blot - Anti-RPLP0 antibody [EP15646] (ab192866)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-RPLP0 antibody [EP15646] (ab192866) at 1/1000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: 3T3-L1 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 3: C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 34 kDa

    Exposure time: 3s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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