Anti-RPLP0 antibody [EP15646]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] (AB192866)

Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling RPLP0 with ab192866 at 1/100 (11.56 ug/ml) dilution following a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human breast

The sections were using heat mediated antigen retrieval with Tris-EDTA (pH 9.0, epitope retrieval solution 2) for 20 mins, then labeling ab192866 at 1/100 dilution for 30 mins at room temperature.
Counterstained with hematoxylin.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] (AB192866)

Immunohistochemical analysis of paraffin embedded Human stomach tissue sections labeling RPLP0 using ab192866 at a 1/100 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RPLP0 antibody [EP15646] (AB192866)

Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa cells labeling RPLP0 with ab192866 at a 1/1000 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary at a 1/400 dilution. Counterstain DAPI. Cells were permeabilized using 0.1% Triton X-100.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] (AB192866)

Immunohistochemical analysis of paraffin embedded rat cerebral cortex tissue sections labeling RPLP0 using ab192866 at a 1/100 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] (AB192866)

Immunohistochemical analysis of paraffin embedded mouse liver tissue sections labeling RPLP0 using ab192866 at a 1/100 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] (AB192866)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling RPLP0 with ab192866 at 1/500 (2.312 ug/ml) dilution following a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse colon

The sections were using heat mediated antigen retrieval with Tris-EDTA (pH 9.0, epitope retrieval solution 2) for 20 mins, then labeling ab192866 at 1/500 dilution for 30 mins at room temperature.
Counterstained with hematoxylin.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] (AB192866)

Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling RPLP0 with ab192866 at 1/500 (2.312 ug/ml) dilution following a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat colon

The sections were using heat mediated antigen retrieval with Tris-EDTA (pH 9.0, epitope retrieval solution 2) for 20 mins, then labeling ab192866 at 1/500 dilution for 30 mins at room temperature.
Counterstained with hematoxylin.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• WB

Lab

Western blot - Anti-RPLP0 antibody [EP15646] (AB192866)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RPLP0 antibody [EP15646] (ab192866) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

3T3-L1 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 3:

C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 34 kDa

false

Exposure time: 3s

• WB

Supplier Data

Western blot - Anti-RPLP0 antibody [EP15646] (AB192866)

All lanes:

Western blot - Anti-RPLP0 antibody [EP15646] (ab192866) at 1/1000 dilution

Lane 1:

Mouse kidney lysate at 10 µg

Lane 2:

Mouse spleen lysate at 10 µg

Lane 3:

Rat brain lysate at 10 µg

Lane 4:

Rat spleen lysate at 10 µg

Lane 5:

C6 cell lysate at 10 µg

Lane 6:

RAW264.7 cell lysate at 10 µg

Lane 7:

PC12 cell lysate at 10 µg

Lane 8:

NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 34 kDa

Observed band size: 34 kDa

false

• WB

Supplier Data

Western blot - Anti-RPLP0 antibody [EP15646] (AB192866)

All lanes:

Western blot - Anti-RPLP0 antibody [EP15646] (ab192866) at 1/20000 dilution

Lane 1:

293 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

A375 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 34 kDa

Observed band size: 34 kDa

false