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Rabbit Recombinant Monoclonal RPLP0 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] - BSA and Azide free (AB236120), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] - BSA and Azide free (AB236120), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] - BSA and Azide free (AB236120), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-RPLP0 antibody [EP15646] - BSA and Azide free (AB236120), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] - BSA and Azide free (AB236120), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-P
Human
Tested
Not recommended
Tested
Mouse
Tested
Not recommended
Tested
Rat
Tested
Not recommended
Tested

Tested
Tested

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

-

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Rat, Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

-

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

1 product for Alternative Version

Target data

Function

Ribosomal protein P0 is the functional equivalent of E.coli protein L10.

Alternative names

60S acidic ribosomal protein P0, 60S ribosomal protein L10E, Large ribosomal subunit protein uL10, RPLP0

Recommended products

Rabbit Recombinant Monoclonal RPLP0 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EP15646

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab236120 is the carrier-free version of Anti-RPLP0 antibody [EP15646] ab192866.

The production method for this product has been changed from hybridoma to recombinant on 18th June 2024.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

RPLP0 also known as 60S acidic ribosomal protein P0 is an integral component of the ribosomal machinery. It acts as a scaffold within the large 60S subunit of the ribosome which plays a fundamental role in protein synthesis. The protein has an approximate mass of 34.4 kDa. RPLP0 is ubiquitously expressed across various tissues indicating its essential role in cellular biology. Its expression is well-documented in highly proliferative tissues and organs reflecting the ribosome's central role in maintaining cellular growth and division.

Biological function summary

RPLP0 participates actively in ribosome structure and function. It forms part of the ribosomal stalk together with the P1 and P2 proteins facilitating interactions with GTPase translation factors during protein synthesis. This ribosomal complex is important for elongation phase of protein translation where it actively assists in the accurate and efficient elongation of polypeptide chains. By participating in the ribosome's structural framework RPLP0 drives the translation process influencing protein output in cells. This makes it essential for overall protein homeostasis.

Pathways

RPLP0 is deeply embedded within the processes of translation and ribosome biogenesis. It associates with the major pathway of ribosome assembly wherein new ribosomes are synthesized and configured. It functions alongside other ribosomal proteins like RPLP1 and RPLP2 which work together to maintain efficient protein synthesis. The interaction among these proteins within the translation pathway highlights the integrated nature of RPLP0 in facilitating cellular protein production.

Associated diseases and disorders

RPLP0 has notable implications in cancer and Diamond-Blackfan anemia (DBA). Aberrations in ribosomal proteins including RPLP0 have been linked to oncogenesis where disrupted protein synthesis contributes to uncontrolled cellular proliferation. Additionally alterations in RPLP0 have associations with DBA a ribosomopathy characterized by defective red blood cell production. Within these disease contexts RPLP0's interaction with other ribosomal proteins like RPL11 can influence disease progression and cellular anomalies seen in such conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] - BSA and Azide free (ab236120), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] - BSA and Azide free (ab236120)

    Immunohistochemical analysis of paraffin embedded Human stomach tissue sections labeling RPLP0 using Anti-RPLP0 antibody [EP15646] ab192866 at a 1/100 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RPLP0 antibody [EP15646] ab192866).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] - BSA and Azide free (ab236120), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] - BSA and Azide free (ab236120)

    Immunohistochemical analysis of paraffin embedded rmouse liver tissue sections labeling RPLP0 using Anti-RPLP0 antibody [EP15646] ab192866 at a 1/100 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RPLP0 antibody [EP15646] ab192866).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] - BSA and Azide free (ab236120), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] - BSA and Azide free (ab236120)

    Immunohistochemical analysis of paraffin embedded rat cerebral cortex tissue sections labeling RPLP0 using Anti-RPLP0 antibody [EP15646] ab192866 at a 1/100 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RPLP0 antibody [EP15646] ab192866).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-RPLP0 antibody [EP15646] - BSA and Azide free (ab236120), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-RPLP0 antibody [EP15646] - BSA and Azide free (ab236120)

    Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa cells labeling RPLP0 with Anti-RPLP0 antibody [EP15646] ab192866 at a 1/1000 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary at a 1/400 dilution. Counterstain DAPI. Cells were permeabilized using 0.1% Triton X-100.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RPLP0 antibody [EP15646] ab192866).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] - BSA and Azide free (ab236120), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] - BSA and Azide free (ab236120)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RPLP0 antibody [EP15646] ab192866).

    Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling RPLP0 with Anti-RPLP0 antibody [EP15646] ab192866 at 1/500 (2.312 ug/ml) dilution following a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on rat colon

    The sections were using heat mediated antigen retrieval with Tris-EDTA (pH 9.0, epitope retrieval solution 2) for 20 mins, then labeling Anti-RPLP0 antibody [EP15646] ab192866 at 1/100 and 1/500 dilution for 30 mins at room temperature.
    Counterstained with hematoxylin.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Western blot - Anti-RPLP0 antibody [EP15646] - BSA and Azide free (ab236120), expandable thumbnail

    Western blot - Anti-RPLP0 antibody [EP15646] - BSA and Azide free (ab236120)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RPLP0 antibody [EP15646] ab192866).

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-RPLP0 antibody [EP15646] (Anti-RPLP0 antibody [EP15646] ab192866) at 1/1000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: 3T3-L1 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 3: C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 34 kDa

    Exposure time: 3s

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] - BSA and Azide free (ab236120), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] - BSA and Azide free (ab236120)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RPLP0 antibody [EP15646] ab192866).

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling RPLP0 with Anti-RPLP0 antibody [EP15646] ab192866 at 1/500 (2.312 ug/ml) dilution following a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse colon

    The sections were using heat mediated antigen retrieval with Tris-EDTA (pH 9.0, epitope retrieval solution 2) for 20 mins, then labeling Anti-RPLP0 antibody [EP15646] ab192866 at 1/100 and 1/500 dilution for 30 mins at room temperature.
    Counterstained with hematoxylin.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] - BSA and Azide free (ab236120), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP0 antibody [EP15646] - BSA and Azide free (ab236120)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RPLP0 antibody [EP15646] ab192866).

    Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling RPLP0 with Anti-RPLP0 antibody [EP15646] ab192866 at 1/100 (11.56 ug/ml) dilution following a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human breast

    The sections were using heat mediated antigen retrieval with Tris-EDTA (pH 9.0, epitope retrieval solution 2) for 20 mins, then labeling Anti-RPLP0 antibody [EP15646] ab192866 at 1/100 and 1/500 dilution for 30 mins at room temperature.
    Counterstained with hematoxylin.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

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Product protocols

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