AB121190

Anti-RPLP1 antibody

Name: Anti-RPLP1 antibody (ab121190) | Abcam
Brand: Abcam Limited
SKU: AB121190
Price: 645 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal RPLP1 antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RPLP1 aa 1 to C-terminus.

View Alternative Names

RRP1, RPLP1, Large ribosomal subunit protein P1, 60S acidic ribosomal protein P1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP1 antibody (AB121190)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP1 antibody (AB121190)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue labelling RPLP1 with ab121190 at 1/200 dilution. Heat mediated antigen retrieval with citrate buffer pH 6 performed before commencing with IHC staining protocol.

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP1 antibody (AB121190)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue labelling RPLP1 with ab121190 at 1/200 dilution. Heat mediated antigen retrieval with citrate buffer pH 6 performed before commencing with IHC staining protocol.

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP1 antibody (AB121190)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling RPLP1 with ab121190 at 1/200 dilution. Heat mediated antigen retrieval with citrate buffer pH 6 performed before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP1 antibody (AB121190)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebellum tissue labelling RPLP1 with ab121190 at 1/200 dilution. Heat mediated antigen retrieval with citrate buffer pH 6 performed before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-RPLP1 antibody (AB121190)

Remaining relative intensity is presented.

Loading control : Anti-GAPDH.

All lanes:

Western blot - Anti-RPLP1 antibody (ab121190) at 0.4 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) transfected with control siRNA, whole cell lysate

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) transfected with RPLP1 specific siRNA probe #1, whole cell lysate

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) transfected with RPLP1 specific siRNA probe #2, whole cell lysate

Predicted band size: 12 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC, WB

applications

Immunogen

Recombinant Fragment Protein within Human RPLP1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P05386

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "0.25-2 µg/mL", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPLP1 also known as the Ribosomal Protein Lateral Stalk Subunit P1 plays an essential mechanical role in protein synthesis within the cell. It is a part of the larger ribosomal 60S subunit and helps in the binding of elongation factors during translation. RPLP1 has a molecular mass of approximately 12 kDa. Its expression is most evident in structures requiring active protein production such as the liver kidney and cardia muscles. Nucleolar localization suggests a role in ribosome biogenesis.

Biological function summary

The protein is involved in regulating ribosome activity for protein synthesis. RPLP1 is a component of the ribosomal stalk which forms an important part of the ribosome complex. It functions with other ribosomal proteins to facilitate the interaction of the ribosome with translational GTPases. The dynamic ability of RPLP1 to dissociate and associate with the ribosome allows the adjustment of translation rates facilitating accurate response to cellular metabolic demands.

Pathways

RPLP1 plays a significant role in the eukaryotic translation pathway including the initiation elongation and termination phases. One of the key interactions is with elongation factors such as EF-2 which helps in the translocation step. This connection highlights RPLP1's role in controlling translational efficiency. Additionally the protein's involvement in the mTOR signaling pathway further emphasizes its importance in cell growth and proliferation relating it to proteins such as mTOR itself and ribosomal proteins like RPL12.

Alterations in RPLP1 expression are associated with certain types of cancer including liver and colorectal cancers. Abnormal RPLP1 activity can disrupt normal protein synthesis rates leading to uncontrolled cell proliferation. This dysfunction interacts with other ribosomal proteins like RPLP2 potentially contributing to tumorigenesis. Furthermore some neurodevelopmental disorders may also link to RPLP1 mutations where it might engage with different proteins involved in neural development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Plays an important role in the elongation step of protein synthesis.

See full target information RPLP1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 27:335-348 PubMed35024245

2022

Mechanism of action of hepatitis B virus S antigen transport-inhibiting oligonucleotide polymer, STOPS, molecules.

Applications

Unspecified application

Species

Unspecified reactive species

C Cheng Kao,Yuchun Nie,Suping Ren,N Tilani T S De Costa,Rajendra K Pandey,Jin Hong,David B Smith,Julian A Symons,Leonid Beigelman,Lawrence M Blatt

Nucleic acids research 48:9872-9885 PubMed32890404

2020

Ribosomal stalk proteins RPLP1 and RPLP2 promote biogenesis of flaviviral and cellular multi-pass transmembrane proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Rafael K Campos,H R Sagara Wijeratne,Premal Shah,Mariano A Garcia-Blanco,Shelton S Bradrick

Cancer cell international 18:170 PubMed30386179

2018

RPLP1 promotes tumor metastasis and is associated with a poor prognosis in triple-negative breast cancer patients.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixian He,Qian Xu,Xi Wang,Jun Wang,Xiangming Mu,Yunhui Cai,Yangyang Qian,Weiwei Shao,Zhimin Shao

Journal of virology 91: PubMed27974556

2017

RPLP1 and RPLP2 Are Essential Flavivirus Host Factors That Promote Early Viral Protein Accumulation.

Applications

Unspecified application

Species

Unspecified reactive species

Rafael K Campos,Benjamin Wong,Xuping Xie,Yi-Fan Lu,Pei-Yong Shi,Julien Pompon,Mariano A Garcia-Blanco,Shelton S Bradrick

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPLP1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP1 antibody (AB121190)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP1 antibody (AB121190)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP1 antibody (AB121190)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP1 antibody (AB121190)

Western blot - Anti-RPLP1 antibody (AB121190)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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