AB154958

Anti-RPLP2 antibody

Name: Anti-RPLP2 antibody (ab154958) | Abcam
Brand: Abcam Limited
SKU: AB154958

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(5 Publications)

Rabbit Polyclonal RPLP2 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RPLP2 aa 1-100.

View Alternative Names

D11S2243E, RPP2, RPLP2, Large ribosomal subunit protein P2, 60S acidic ribosomal protein P2, Renal carcinoma antigen NY-REN-44

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-RPLP2 antibody (AB154958)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RPLP2 antibody (AB154958)

Immunofluorescence analysis of methanol fixed A431 cells labeling RPLP2 with ab154958 at 1/500. Lower panel shows costaining with Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP2 antibody (AB154958)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP2 antibody (AB154958)

Immunohistochemical analysis of paraffin embedded Human colon carcinoma tissue labeling RPLP2 with ab154958 at 1/500.

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Western blot - Anti-RPLP2 antibody (AB154958)

15% SDS PAGE

All lanes:

Western blot - Anti-RPLP2 antibody (ab154958) at 1/1000 dilution

Lane 1:

Molt4 whole cell lysate at 30 µg

Lane 2:

Raji whole cell lysate at 30 µg

Predicted band size: 12 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human RPLP2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P05387

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)." }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPLP2 also known as the Ribosomal Protein Lateral Stalk Subunit P2 is a component of the ribosomal assembly. It has a molecular weight of approximately 11 kDa. This protein performs an integral role in the formation and function of the 60S ribosomal subunit facilitating protein synthesis in the cell. RPLP2 is expressed in various tissues indicating its widespread importance in cellular activities and functions.

Biological function summary

RPLP2 contributes to essential cellular processes by being part of the ribosomal complex. This protein works in concert with other ribosomal P proteins to form the lateral stalk of the ribosome important for translational function. Within the ribosome RPLP2 helps in the binding of elongation factors impacting protein elongation during translation. The interaction of these proteins as part of the ribosome highlights their significance in cell growth and proliferation.

Pathways

RPLP2 plays a significant role in translation and protein synthesis pathways. It is involved in the translation initiation pathway which is important for starting mRNA translation into proteins. In this context RPLP2 interacts with other ribosomal components such as RPLP0 and RPLP1 contributing to the dynamic process of translation. These interactions are critical for the pathway's function ensuring accurate and efficient protein synthesis.

RPLP2 has associations with certain conditions like cancer and ribosomopathies. Abnormal expression or malfunction of RPLP2 may disrupt protein synthesis potentially leading to oncogenesis or growth disorders. Changes in RPLP2 can impact pathways involving related proteins such as other ribosomal proteins which when altered influence disease development. Understanding these connections helps in researching therapeutic interventions targeting protein synthesis dysregulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays an important role in the elongation step of protein synthesis.

See full target information RPLP2

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:5290 PubMed38906865

2024

RPLP1 restricts HIV-1 transcription by disrupting C/EBPβ binding to the LTR.

Applications

Unspecified application

Species

Unspecified reactive species

Weijing Yang,Hong Wang,Zhaolong Li,Lihua Zhang,Jianhui Liu,Frank Kirchhoff,Chen Huan,Wenyan Zhang

Cell death discovery 9:440 PubMed38052785

2023

RPLP2 activates TLR4 in an autocrine manner and promotes HIF-1α-induced metabolic reprogramming in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qingqing Yang,Xiangrui Meng,Jin Chen,Xiangsu Li,Yang Huang,Xueyi Xiao,Rongqing Li,Xudong Wu

Molecular therapy. Nucleic acids 27:335-348 PubMed35024245

2022

Mechanism of action of hepatitis B virus S antigen transport-inhibiting oligonucleotide polymer, STOPS, molecules.

Applications

Unspecified application

Species

Unspecified reactive species

C Cheng Kao,Yuchun Nie,Suping Ren,N Tilani T S De Costa,Rajendra K Pandey,Jin Hong,David B Smith,Julian A Symons,Leonid Beigelman,Lawrence M Blatt

Nucleic acids research 48:9872-9885 PubMed32890404

2020

Ribosomal stalk proteins RPLP1 and RPLP2 promote biogenesis of flaviviral and cellular multi-pass transmembrane proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Rafael K Campos,H R Sagara Wijeratne,Premal Shah,Mariano A Garcia-Blanco,Shelton S Bradrick

Journal of virology 91: PubMed27974556

2017

RPLP1 and RPLP2 Are Essential Flavivirus Host Factors That Promote Early Viral Protein Accumulation.

Applications

Unspecified application

Species

Unspecified reactive species

Rafael K Campos,Benjamin Wong,Xuping Xie,Yi-Fan Lu,Pei-Yong Shi,Julien Pompon,Mariano A Garcia-Blanco,Shelton S Bradrick

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPLP2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-RPLP2 antibody (AB154958)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP2 antibody (AB154958)

Western blot - Anti-RPLP2 antibody (AB154958)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise