AB317179

Anti-RPLP2 antibody [HL2256]

Recombinant
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Rabbit Recombinant Monoclonal RPLP2 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse, Rat, Zebrafish samples. Immunogen corresponding to Recombinant Fragment Protein within Human RPLP2.

View Alternative Names

D11S2243E, RPP2, RPLP2, Large ribosomal subunit protein P2, 60S acidic ribosomal protein P2, Renal carcinoma antigen NY-REN-44

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP2 antibody [HL2256] (AB317179)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP2 antibody [HL2256] (AB317179)

ab317179 detects secreted RPLP2 protein by immunohistochemical analysis. Sample : Paraffin-embedded human colon cancer. RPLP2 stained by ab317179 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Immunocytochemistry/ Immunofluorescence - Anti-RPLP2 antibody [HL2256] (AB317179)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RPLP2 antibody [HL2256] (AB317179)

ab317179 detects RPLP2 protein at cytoplasm by immunofluorescent analysis. Sample : A431 cells were fixed in ice-cold MeOH for 5 min. Green : RPLP2 stained by ab317179 diluted at 1 : 500. Blue : Fluoroshield with DAPI.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPLP2 antibody [HL2256] (AB317179)

ab317179 detects RPLP2 protein by immunohistochemical analysis. Sample : Paraffin-embedded mouse tissues. RPLP2 stained by ab317179 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Supplier Data

Western blot - Anti-RPLP2 antibody [HL2256] (AB317179)

Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE and the membrane was blotted with ab317179 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-RPLP2 antibody [HL2256] (ab317179) at 1/1000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Lane 3:

HeLa whole cell extracts at 30 µg

Lane 4:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Supplier Data

Western blot - Anti-RPLP2 antibody [HL2256] (AB317179)

Rat tissue extract (50 ug) was separated by 15% SDS-PAGE and the membrane was blotted with ab317179 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-RPLP2 antibody [HL2256] (ab317179) at 1/1000 dilution

All lanes:

Rat kidney tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

Supplier Data

Western blot - Anti-RPLP2 antibody [HL2256] (AB317179)

Whole cell extract (30 ug) was separated by 15% SDS-PAGE and the membrane was blotted with ab317179 diluted at 1 : 20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-RPLP2 antibody [HL2256] (ab317179) at 1/20000 dilution

All lanes:

Whole zebrafish extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2256

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat, Zebrafish

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human RPLP2.

P05387

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/20000", "WB-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPLP2 also known as the Ribosomal Protein Lateral Stalk Subunit P2 is a component of the ribosomal assembly. It has a molecular weight of approximately 11 kDa. This protein performs an integral role in the formation and function of the 60S ribosomal subunit facilitating protein synthesis in the cell. RPLP2 is expressed in various tissues indicating its widespread importance in cellular activities and functions.

Biological function summary

RPLP2 contributes to essential cellular processes by being part of the ribosomal complex. This protein works in concert with other ribosomal P proteins to form the lateral stalk of the ribosome important for translational function. Within the ribosome RPLP2 helps in the binding of elongation factors impacting protein elongation during translation. The interaction of these proteins as part of the ribosome highlights their significance in cell growth and proliferation.

Pathways

RPLP2 plays a significant role in translation and protein synthesis pathways. It is involved in the translation initiation pathway which is important for starting mRNA translation into proteins. In this context RPLP2 interacts with other ribosomal components such as RPLP0 and RPLP1 contributing to the dynamic process of translation. These interactions are critical for the pathway's function ensuring accurate and efficient protein synthesis.

RPLP2 has associations with certain conditions like cancer and ribosomopathies. Abnormal expression or malfunction of RPLP2 may disrupt protein synthesis potentially leading to oncogenesis or growth disorders. Changes in RPLP2 can impact pathways involving related proteins such as other ribosomal proteins which when altered influence disease development. Understanding these connections helps in researching therapeutic interventions targeting protein synthesis dysregulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays an important role in the elongation step of protein synthesis.

See full target information RPLP2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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