AB244399

Anti-RPN2 antibody

Name: Anti-RPN2 antibody (ab244399) | Abcam
Brand: Abcam Limited
SKU: AB244399
Price: 635 USD
Availability: InStock

Be the first to review this product! Submit a review

(6 Publications)

Rabbit Polyclonal RPN2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 aa 300-500.

View Alternative Names

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit, RIBIIR, Ribophorin II, Ribophorin-2, RPN-II, RPN2

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-RPN2 antibody (AB244399)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RPN2 antibody (AB244399)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for RPN2 (green) using ab244399 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPN2 antibody (AB244399)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPN2 antibody (AB244399)

Paraffin-embedded human epididymis tissue stained for RPN2 using ab244399 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPN2 antibody (AB244399)

Paraffin-embedded human skeletal muscle tissue stained for RPN2 using ab244399 at 1/50 dilution in immunohistochemical analysis.

Low expression as expected.

Unknown

Western blot - Anti-RPN2 antibody (AB244399)

All lanes:

Western blot - Anti-RPN2 antibody (ab244399) at 0.4 µg/mL

Lane 1:

RT4 (Human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG (Human brain glioma cell line) cell lysate

Predicted band size: 69 kDa

false

CiteAb

Western blot - Anti-RPN2 antibody (AB244399)

RPN2 western blot using anti-RPN2 antibody ab244399. Publication image and figure legend from Ding, C., Wu, Z., et al., 2019, J Exp Clin Cancer Res, PubMed 31888753.

ab244399 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab244399 please see the product overview.

Silence of circNFIX decreases xenograft tumor growth by regulating miR-378e/RPN2 axis. T98 and U251 cells were stably transfected with sh-NC or sh-circNFIX and then infected into nude mice. (a-c) Tumor volume and weight were measured. (d-f) The expression levels of circNFIX, miR-378e and RPN2 protein were measured in tumor tissues. **p < 0.01, ***p < 0.001 compared with sh-NC group

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 aa 300-500. The exact immunogen used to generate this antibody is proprietary information.

P04844

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPN2 also known as Ribophorin II is a subunit of the oligosaccharyltransferase (OST) complex. This protein has a mass of approximately 68 kDa. It is expressed mainly in the rough endoplasmic reticulum playing a role in the N-linked glycosylation of nascent polypeptides. This process is essential for protein folding and stability. RPN2 helps transfer oligosaccharide chains to asparagine residues in nascent proteins.

Biological function summary

RPN2 is integral to the OST complex which consists of multiple subunits each contributing to the glycosylation process. By enabling glycosylation RPN2 ensures proper protein folding allowing proteins to achieve functional conformation and maintain stability. This process affects various cellular activities including protein secretion cell surface receptor expression and immune function.

Pathways

RPN2 plays a significant role in the N-linked glycosylation pathway. This pathway involves the attachment of glycans to proteins influencing their folding distribution and function. RPN2 interacts with other proteins such as STT3A and STT3B which are essential components of the OST complex. This interaction modulates protein modification during synthesis and affects downstream pathways like cell signaling and immune response.

RPN2 has connections to cancer and congenital disorders of glycosylation (CDG). Its altered expression in cancer cells correlates with tumor progression and metastasis where it may impact the function of proteins such as EGFR involved in cell growth and survival. In CDG deficiencies in glycosylation due to RPN2 malfunction can lead to a range of symptoms affecting multiple body systems linking it with several glycosylation-related proteins.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation (PubMed : 31831667). N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.

See full target information Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2

style

left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cancer science 114:490-503 PubMed36227125

2022

circTOP2A functions as a ceRNA to promote glioma progression by upregulating RPN2.

Applications

Unspecified application

Species

Unspecified reactive species

Jikui Sun,Jinhuan Wang,Meng Li,Shengjie Li,Hanyun Li,Yan Lu,Feng Li,Tao Xin,Feng Jin

Theranostics 12:6363-6379 PubMed36168628

2022

The N-methyladenosine-mediated lncRNA WEE2-AS1 promotes glioblastoma progression by stabilizing RPN2.

Applications

Unspecified application

Species

Unspecified reactive species

Boyan Li,Rongrong Zhao,Wei Qiu,Ziwen Pan,Shulin Zhao,Yanhua Qi,Jiawei Qiu,Shouji Zhang,Qindong Guo,Yang Fan,Hao Xu,Ming Li,Gang Li,Hao Xue

Bioengineered 13:5141-5151 PubMed35156537

2022

Ribophorin II promotes the epithelial-mesenchymal transition and aerobic glycolysis of laryngeal squamous cell carcinoma via regulating reactive oxygen species-mediated Phosphatidylinositol-3-Kinase/Protein Kinase B activation.

Applications

Unspecified application

Species

Unspecified reactive species

Jingchun Zhou,Jingjing Zhang,Wei Zhang,Zhaoyang Ke,Yanlu Lv,Bo Zhang,Zhifang Liao

International journal of molecular medicine 47:643-658 PubMed33416146

2021

Regulatory role of DEPTOR‑mediated cellular autophagy and mitochondrial reactive oxygen species in angiogenesis in multiple myeloma.

Applications

Unspecified application

Species

Unspecified reactive species

Jizhen Wang,Junmin Chen,Dongbiao Qiu,Zhiyong Zeng

Oncology reports 44:2108-2120 PubMed33000268

2020

MicroRNA‑422a functions as a tumor suppressor in glioma by regulating the Wnt/β‑catenin signaling pathway via RPN2.

Applications

Unspecified application

Species

Unspecified reactive species

Jikui Sun,Zhijuan Chen,Jinbiao Xiong,Qiong Wang,Fan Tang,Xuebin Zhang,Lidong Mo,Chen Wang,Weijia Fan,Jinhuan Wang

Journal of experimental & clinical cancer research 38:506 PubMed31888753

2019

CircNFIX promotes progression of glioma through regulating miR-378e/RPN2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Chenyu Ding,Zanyi Wu,Honghai You,Hongliang Ge,Shufa Zheng,Yuanxiang Lin,Xiyue Wu,Zhangya Lin,Dezhi Kang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPN2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-RPN2 antibody (AB244399)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPN2 antibody (AB244399)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPN2 antibody (AB244399)

Western blot - Anti-RPN2 antibody (AB244399)

Western blot - Anti-RPN2 antibody (AB244399)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise