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AB126338

Anti-RPP25 antibody

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(1 Publication)

Rabbit Polyclonal RPP25 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Ribonuclease P protein subunit p25.

View Alternative Names

Ribonuclease P protein subunit p25, RNase P protein subunit p25, RPP25

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPP25 antibody (AB126338)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPP25 antibody (AB126338)

Immunohistochemical analysis of human skeletal muscle tissue labeling RPR25 with ab126338 at a 1/50 dilution. No positivity as expected.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-RPP25 antibody (AB126338)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RPP25 antibody (AB126338)

Immunocytochemical analysis of SiHa (Human cervical carcinoma cell line) whole cells labeling RPR25 in the nucleoplasm and microtubule organizing center with ab126338 at 2 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPP25 antibody (AB126338)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPP25 antibody (AB126338)

Immunohistochemical analysis of human colon tissue labeling RPR25 in the nucleus of glandular cells with ab126338 at a 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPP25 antibody (AB126338)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPP25 antibody (AB126338)

Immunohistochemical analysis of human kidney tissue labeling RPR25 in the nucleus of cells in tubules with ab126338 at a 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPP25 antibody (AB126338)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPP25 antibody (AB126338)

Immunohistochemical analysis of human testis tissue labeling RPR25 in the nucleus of Leydig cells with ab126338 at a 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-RPP25 antibody (AB126338)
  • WB

Supplier Data

Western blot - Anti-RPP25 antibody (AB126338)

All lanes:

Western blot - Anti-RPP25 antibody (ab126338) at 0.4 µg/mL

All lanes:

SK-MEL-30 (Human cutaneous melanoma cell line) whole cell lysate

Predicted band size: 21 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human Ribonuclease P protein subunit p25. The exact immunogen used to generate this antibody is proprietary information.

Q9BUL9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPP25 also known as Ribonuclease P/MRP 25 kDa subunit is a protein with an approximate mass of 25 kDa. It plays an important mechanical role in the cellular environment as part of the ribonuclease P and ribonuclease MRP complexes. These complexes are key for the cleavage of precursor RNAs. RPP25 is expressed broadly with presence noted in most human tissues which suggests its functional importance in various cellular processes.
Biological function summary

RPP25 functions as an essential part of ribonucleoprotein complexes that include ribonuclease P and ribonuclease MRP. These complexes are involved in the maturation of tRNA and rRNA respectively highlighting RPP25's role in RNA processing. As a component of these ribonucleases RPP25 works alongside other proteins such as RPP30 and POP1 helping to perform critical RNA processing tasks within the cell.

Pathways

The involvement of RPP25 in the RNA processing pathway demonstrates its importance alongside other proteins. In particular it takes part in the maturation of tRNA within the tRNA biosynthesis pathway. This process is critical for proper translation and protein synthesis. RPP25 also interacts within the rRNA processing pathway where it contributes to the generation of mature ribosomal RNA a necessity for the formation of functional ribosomes.

Mutations or dysregulation of RPP25 are linked to ribosomopathies and possibly certain cancers. For example abnormal expression levels might have connections to Diamond-Blackfan anemia a disorder arising from defects in ribosomal function. In this context RPP25 may interact with proteins like RPL5 which is also associated with ribosomal biogenesis and linked diseases pointing to a potential pathway for therapeutic exploration.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of ribonuclease P, a ribonucleoprotein complex that generates mature tRNA molecules by cleaving their 5'-ends (PubMed : 12003489, PubMed : 16723659, PubMed : 30454648). Also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences (PubMed : 28115465).
See full target information Ribonuclease P protein subunit p25
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:681 PubMed39289348

2024

MANF serves as a novel hepatocyte factor to promote liver regeneration after 2/3 partial hepatectomy via doubly targeting Wnt/β-catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yanyan Liang,Qiong Mei,Enguang He,Petek Ballar,Chuansheng Wei,Yue Wang,Yue Dong,Jie Zhou,Xiaofang Tao,Wenyan Qu,Mingxia Zhao,Goma Chhetri,Limeng Wei,Juntang Shao,Yujun Shen,Jun Liu,Lijie Feng,Yuxian Shen
View all publications
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Product promise

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