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AB53744

Anti-RPP30 antibody [2931D5a]

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(1 Publication)

Mouse Monoclonal RPP30 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RPP30.

View Alternative Names

RNASEP2, RPP30, Ribonuclease P protein subunit p30, RNaseP protein p30, RNase P subunit 2

2 Images
Western blot - Anti-RPP30 antibody [2931D5a] (AB53744)
  • WB

Unknown

Western blot - Anti-RPP30 antibody [2931D5a] (AB53744)

All lanes:

Western blot - Anti-RPP30 antibody [2931D5a] (ab53744) at 1/100 dilution

All lanes:

HeLa whole cell lysate at 25 µg

Secondary

All lanes:

anti Mouse IgG at 1/2500 dilution

Predicted band size: 29 kDa

Observed band size: 30 kDa

true

Western blot - Anti-RPP30 antibody [2931D5a] (AB53744)
  • WB

Unknown

Western blot - Anti-RPP30 antibody [2931D5a] (AB53744)

All lanes:

Western blot - Anti-RPP30 antibody [2931D5a] (ab53744) at 1/100 dilution

All lanes:

NIH3T3 whole cell lysate at 25 µg

Secondary

All lanes:

anti Mouse IgG at 1/2500 dilution

Predicted band size: 29 kDa

Observed band size: 30 kDa

true

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2931D5a

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human RPP30. The exact immunogen used to generate this antibody is proprietary information.

P78346

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum.
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPP30 also known as Ribonuclease P protein subunit p30 is a protein involved in the enzymatic process that removes 5' leader sequences from precursor tRNA molecules. It has a mass of approximately 25 kDa. RPP30 is expressed in various tissues across the human body indicating its fundamental role in RNA processing.
Biological function summary

RPP30 plays a role as part of the Ribonuclease P complex which is important for the maturation of tRNA. The Ribonuclease P complex contains both RNA and protein components and RPP30 interacts with other subunits to form the complete ribonucleoprotein complex. This association is necessary for catalytic activity and stability of the complex.

Pathways

RPP30 is involved in RNA processing pathways important for cell viability. Specifically it is part of the tRNA processing pathway working alongside proteins like RPP14 and POP1 to assist in the maturation of tRNA. These interactions emphasize the integration of RPP30 in important cellular processes that affect protein synthesis.

Involvement of RPP30 in certain autoimmune conditions has been noted. Altered expression of RPP30 and its associated complex proteins can contribute to autoimmune diseases like systemic sclerosis. Additionally aberrations in RPP30 expression or function have been linked with alterations in RNA processing pathways that may impact cell proliferation and survival further showing connections to these disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of ribonuclease P, a ribonucleoprotein complex that generates mature tRNA molecules by cleaving their 5'-ends (PubMed : 30454648, PubMed : 9037013, PubMed : 9630247). Also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences (PubMed : 28115465).
See full target information RPP30
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer management and research 12:8253-8261 PubMed32982421

2020

LncRNA GAS5 Regulates Osteosarcoma Cell Proliferation, Migration, and Invasion by Regulating RHOB via Sponging miR-663a.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyu Yao,Xianan Li,Yi Luo,Xuezheng Xu,Jianfan Liu,Jie Bu
View all publications
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Product promise

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