Anti-RPS10 antibody [EPR8545]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS10 antibody [EPR8545] (AB151550)

Immunohistochemical analysis of paraffin embedded Human Lung carcinoma tissue using ab151550 showing +ve staining.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS10 antibody [EPR8545] (AB151550)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human pancreas tissue sections labeling RPS10 with Purified ab151550 at 1 : 100 dilution (11.86 μg/ml). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS10 antibody [EPR8545] (AB151550)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling RPS10 with ab151550 at 1/50 dilution.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-RPS10 antibody [EPR8545] (AB151550)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling RPS10 with Purified ab151550 at 1/1000 dilution (1μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS10 antibody [EPR8545] (AB151550)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human gastric carcinoma tissue sections labeling RPS10 with Purified ab151550 at 1 : 100 dilution (11.86 μg/ml). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS10 antibody [EPR8545] (AB151550)

Immunohistochemical analysis of paraffin embedded Human Colonic adenocarcinoma tissue using ab151550 showing +ve staining.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS10 antibody [EPR8545] (AB151550)

Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab151550 showing +ve staining.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS10 antibody [EPR8545] (AB151550)

Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab151550 showing +ve staining.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RPS10 antibody [EPR8545] (AB151550)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling RPS10 with Purified ab151550 at 1 : 250 dilution (4.7 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RPS10 antibody [EPR8545] (AB151550)

Immunofluorescent analysis of HeLa cells labelling RPS10 with ab151550 at 1/250 dilution.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS10 antibody [EPR8545] (AB151550)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat stomach tissue sections labeling RPS10 with Purified ab151550 at 1 : 100 dilution (11.86 μg/ml). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS10 antibody [EPR8545] (AB151550)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse stomach tissue sections labeling RPS10 with Purified ab151550 at 1 : 100 dilution (11.86 μg/ml). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• WB

Lab

Western blot - Anti-RPS10 antibody [EPR8545] (AB151550)

The blocking and dilution buffer is : 5% NFDM

All lanes:

Western blot - Anti-RPS10 antibody [EPR8545] (ab151550) at 1/5000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates at 20 µg

Lane 3:

Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates at 20 µg

Lane 4:

C6 (Rat glial tumor glial cell) whole cell lysates at 20 µg

Lane 5:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates at 20 µg

Lane 6:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 20 µg

Lane 7:

PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 19 kDa

Observed band size: 19 kDa

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• WB

Unknown

Western blot - Anti-RPS10 antibody [EPR8545] (AB151550)

All lanes:

Western blot - Anti-RPS10 antibody [EPR8545] (ab151550) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

Raji cell lysate at 10 µg

Lane 4:

C6 cell lysate at 10 µg

Lane 5:

Raw264.7 cell lysate at 10 µg

Lane 6:

PC12 cell lysate at 10 µg

Lane 7:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 19 kDa

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• WB

CiteAb

Western blot - Anti-RPS10 antibody [EPR8545] (AB151550)

RPS10 western blot using anti-RPS10 antibody [EPR8545] ab151550. Publication image and figure legend from Haneke, K., Schott, J., et al., 2020, J Cell Biol, PubMed 32040547.

ab151550 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab151550 please see the product overview.

LARP1-dependent suppression of 5′TOP mRNA translation upon CDK1i. (A) For Ribo-Seq analysis, RPE1 cells were serum-starved for 48 h followed by a 4-h treatment with DMSO or Ro3306 (10 µM). Equal amounts of a yeast lysate were spiked into the DMSO- and Ro3306-treated samples. RDs (no. ribosome footprints/no. ORF-spanning reads in input RNA) were calculated after normalization to the yeast spike-in footprints from n = 3 biological repeat experiments. (B) Based on the Ribo-Seq analysis in A, the average RD was calculated after normalization (norm.) to the yeast spike-in. Statistical significance was determined by one-tailed ratio paired t test. (C) Based on the Ribo-Seq analysis in A, the fold change in RD (Δ RD) was calculated for IRES-containing mRNAs, 5′TOP mRNAs, and all other mRNAs. (D) Polysome association of 5′TOP (RPLP0 and PABPC4) and ORF size-matched non-TOP (EIF2S1 and NCL) mRNAs was analyzed by polysome fractionation and subsequent qPCR analysis from DMSO- or Ro3306-treated (10 µM, 4 h) HeLa cells. (E) Western blot analysis of LARP1 expression in HEK293T WT cells, HEK293T LARP1−/− cells, and HEK293T LARP1−/− + LARP1 cells expressing LARP1-Flag-SBP. RPS10 serves as loading control. (F) Polysome association of 5′TOP (RPS6 and RPS7) and ORF size-matched non-TOP (EIF2S1, CDKN1A) mRNAs was analyzed by polysome fractionation and subsequent qPCR analysis from DMSO- or Ro3306-treated (10 µM, 4 h) HEK293T WT, LARP1−/−, or LARP1−/− + LARP1 cells.

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