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AB249870

Anti-RPS12 antibody [EPR10963] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal RPS12 antibody. Carrier free. Suitable for WB, ICC/IF, IP, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

Small ribosomal subunit protein eS12, 40S ribosomal protein S12, RPS12

4 Images
Flow Cytometry (Intracellular) - Anti-RPS12 antibody [EPR10963] - BSA and Azide free (AB249870)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-RPS12 antibody [EPR10963] - BSA and Azide free (AB249870)

This data was developed using ab175219, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized HeLa cells labeling RPS12 with ab175219 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

Immunocytochemistry/ Immunofluorescence - Anti-RPS12 antibody [EPR10963] - BSA and Azide free (AB249870)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RPS12 antibody [EPR10963] - BSA and Azide free (AB249870)

This data was developed using ab175219, the same antibody clone in a different buffer formulation.

Immunofluorescent staining of MCF7 cells labeling RPS12 with ab175219 at 1/50 dilution (green). DAPI nuclear staining (blue).

Immunoprecipitation - Anti-RPS12 antibody [EPR10963] - BSA and Azide free (AB249870)
  • IP

Supplier Data

Immunoprecipitation - Anti-RPS12 antibody [EPR10963] - BSA and Azide free (AB249870)

This data was developed using ab175219, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from HeLa cell lysate using ab175219 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-RPS12 antibody [EPR10963] (<a href='/en-us/products/primary-antibodies/rps12-antibody-epr10963-ab175219'>ab175219</a>)

Predicted band size: 15 kDa

false

Western blot - Anti-RPS12 antibody [EPR10963] - BSA and Azide free (AB249870)
  • WB

Supplier Data

Western blot - Anti-RPS12 antibody [EPR10963] - BSA and Azide free (AB249870)

This data was developed using ab175219, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RPS12 antibody [EPR10963] (<a href='/en-us/products/primary-antibodies/rps12-antibody-epr10963-ab175219'>ab175219</a>) at 1/1000 dilution

Lane 1:

Hela cell lysate at 10 µg

Lane 2:

MCF-7 cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

Raji cell lysate at 10 µg

Lane 5:

C6 cell lysate at 10 µg

Lane 6:

RAW 264.7 cell lysate at 10 µg

Lane 7:

NIH/3T3 cell lysate at 10 µg

Predicted band size: 15 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10963

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), WB, ICC/IF, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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  • 4
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab249870 is the carrier-free version of ab175219.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPS12 also known as Ribosomal Protein S12 is a component of the 40S ribosomal subunit. It has a molecular mass of approximately 14 kDa. This protein plays an important role in the synthesis of proteins by helping to translate messenger RNA into polypeptides. It is expressed widely in human tissues indicating its fundamental role in cellular function. RPS12 is an integral part of ribosome structure and functionality contributing to the assembly and stability of the ribosomes.
Biological function summary

Ribosomal Protein S12 is involved in forming the small ribosomal subunit and it is a part of the ribonucleoprotein complex that is responsible for decoding genetic information. This complex ensures the accurate translation of genetic sequences which is essential for proper cell division and growth. The function of RPS12 extends to maintaining the fidelity of translation ensuring that amino acids are added correctly during protein synthesis.

Pathways

The role of Ribosomal Protein S12 is significant in the mRNA translation pathway affecting gene expression and protein production. It is directly linked to the regulation of the translation machinery. RPS12 is associated with various other ribosomal proteins such as RPS6 and RPL5 to form a coordinated network that influences protein synthesis and cellular responses to environmental changes. Its interaction with these proteins underpins its participation in the global control of protein synthesis.

Altered expression or mutations in Ribosomal Protein S12 have connections with certain ribosomopathies. Disorders like Diamond-Blackfan anemia relate to dysfunctions within ribosomal components including RPS12. Moreover aberrations in ribosomal proteins including RPS12 can predispose individuals to cancer by disrupting normal cell growth control. Understanding how RPS12 interacts with proteins involved in these diseases can provide insights into potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797). Subunit of the 40S ribosomal complex (By similarity).
See full target information RPS12
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:775642 PubMed35004677

2021

HIF-1α Induces HECTD2 Up-Regulation and Aggravates the Malignant Progression of Renal Cell Cancer Repressing miR-320a.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Lv,Taimin Shen,Juncheng Yao,Qi Yang,Ying Xiang,Zhiwei Ma
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com