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Rabbit Recombinant Monoclonal RPS12 antibody. Carrier free. Suitable for WB, ICC/IF, IP, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.

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Images

Immunoprecipitation - Anti-RPS12 antibody [EPR10963] - BSA and Azide free (AB249870), expandable thumbnail
  • Western blot - Anti-RPS12 antibody [EPR10963] - BSA and Azide free (AB249870), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-RPS12 antibody [EPR10963] - BSA and Azide free (AB249870), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-RPS12 antibody [EPR10963] - BSA and Azide free (AB249870), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIPIHC-PFlow Cyt (Intra)
Human
Tested
Tested
Tested
Not recommended
Tested
Mouse
Tested
Expected
Expected
Not recommended
Expected
Rat
Tested
Expected
Expected
Not recommended
Expected

Tested
Tested

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed:34516797). Subunit of the 40S ribosomal complex (By similarity).

Alternative names

Small ribosomal subunit protein eS12, 40S ribosomal protein S12, RPS12

Recommended products

Rabbit Recombinant Monoclonal RPS12 antibody. Carrier free. Suitable for WB, ICC/IF, IP, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR10963
Purification technique
Affinity purification
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab249870 is the carrier-free version of Anti-RPS12 antibody [EPR10963] ab175219.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

RPS12 also known as Ribosomal Protein S12 is a component of the 40S ribosomal subunit. It has a molecular mass of approximately 14 kDa. This protein plays an important role in the synthesis of proteins by helping to translate messenger RNA into polypeptides. It is expressed widely in human tissues indicating its fundamental role in cellular function. RPS12 is an integral part of ribosome structure and functionality contributing to the assembly and stability of the ribosomes.

Biological function summary

Ribosomal Protein S12 is involved in forming the small ribosomal subunit and it is a part of the ribonucleoprotein complex that is responsible for decoding genetic information. This complex ensures the accurate translation of genetic sequences which is essential for proper cell division and growth. The function of RPS12 extends to maintaining the fidelity of translation ensuring that amino acids are added correctly during protein synthesis.

Pathways

The role of Ribosomal Protein S12 is significant in the mRNA translation pathway affecting gene expression and protein production. It is directly linked to the regulation of the translation machinery. RPS12 is associated with various other ribosomal proteins such as RPS6 and RPL5 to form a coordinated network that influences protein synthesis and cellular responses to environmental changes. Its interaction with these proteins underpins its participation in the global control of protein synthesis.

Associated diseases and disorders

Altered expression or mutations in Ribosomal Protein S12 have connections with certain ribosomopathies. Disorders like Diamond-Blackfan anemia relate to dysfunctions within ribosomal components including RPS12. Moreover aberrations in ribosomal proteins including RPS12 can predispose individuals to cancer by disrupting normal cell growth control. Understanding how RPS12 interacts with proteins involved in these diseases can provide insights into potential therapeutic targets.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunoprecipitation - Anti-RPS12 antibody [EPR10963] - BSA and Azide free (ab249870), expandable thumbnail

    Immunoprecipitation - Anti-RPS12 antibody [EPR10963] - BSA and Azide free (ab249870)

    This data was developed using Anti-RPS12 antibody [EPR10963] ab175219, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from HeLa cell lysate using Anti-RPS12 antibody [EPR10963] ab175219 at 1/10 dilution.

    All lanes: Immunoprecipitation - Anti-RPS12 antibody [EPR10963] (Anti-RPS12 antibody [EPR10963] ab175219)

    Predicted band size: 15 kDa

  • Western blot - Anti-RPS12 antibody [EPR10963] - BSA and Azide free (ab249870), expandable thumbnail

    Western blot - Anti-RPS12 antibody [EPR10963] - BSA and Azide free (ab249870)

    This data was developed using Anti-RPS12 antibody [EPR10963] ab175219, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-RPS12 antibody [EPR10963] (Anti-RPS12 antibody [EPR10963] ab175219) at 1/1000 dilution

    Lane 1: Hela cell lysate at 10 µg

    Lane 2: MCF-7 cell lysate at 10 µg

    Lane 3: HepG2 cell lysate at 10 µg

    Lane 4: Raji cell lysate at 10 µg

    Lane 5: C6 cell lysate at 10 µg

    Lane 6: RAW 264.7 cell lysate at 10 µg

    Lane 7: NIH/3T3 cell lysate at 10 µg

    Predicted band size: 15 kDa

  • Flow Cytometry (Intracellular) - Anti-RPS12 antibody [EPR10963] - BSA and Azide free (ab249870), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-RPS12 antibody [EPR10963] - BSA and Azide free (ab249870)

    This data was developed using Anti-RPS12 antibody [EPR10963] ab175219, the same antibody clone in a different buffer formulation.

    Intracellular flow cytometric analysis of permeabilized HeLa cells labeling RPS12 with Anti-RPS12 antibody [EPR10963] ab175219 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

  • Immunocytochemistry/ Immunofluorescence - Anti-RPS12 antibody [EPR10963] - BSA and Azide free (ab249870), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-RPS12 antibody [EPR10963] - BSA and Azide free (ab249870)

    This data was developed using Anti-RPS12 antibody [EPR10963] ab175219, the same antibody clone in a different buffer formulation.Immunofluorescent staining of MCF7 cells labeling RPS12 with Anti-RPS12 antibody [EPR10963] ab175219 at 1/50 dilution (green). DAPI nuclear staining (blue).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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