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AB174661

Anti-RPS14 antibody

5

(1 Review)

|

(4 Publications)

Rabbit Polyclonal RPS14 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human RPS14 aa 100 to C-terminus.

View Alternative Names

PRO2640, RPS14, Small ribosomal subunit protein uS11, 40S ribosomal protein S14

2 Images
Immunoprecipitation - Anti-RPS14 antibody (AB174661)
  • IP

Supplier Data

Immunoprecipitation - Anti-RPS14 antibody (AB174661)

Detection of RPS14 by Western Blot of Immunoprecipitate.<br>Lane 1 : Immunoprecipitation of 293T whole cell lysate using ab174661 at 6μg/mg lysate (1 mg/IP; 20% of IP loaded/lane) followed by detection of RPS14 with ab174661 at 1 μg/ml. The lysate in lane 2 is precipitated with an IgG control antibody.

All lanes:

Immunoprecipitation - Anti-RPS14 antibody (ab174661)

Predicted band size: 16 kDa

true

Exposure time: 10s

Western blot - Anti-RPS14 antibody (AB174661)
  • WB

Supplier Data

Western blot - Anti-RPS14 antibody (AB174661)

All lanes:

Western blot - Anti-RPS14 antibody (ab174661) at 0.1 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

Mouse TCMK-1 whole cell lysate at 50 µg

Lane 5:

Mouse NIH3T3 whole cell lysate at 50 µg

Predicted band size: 16 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human RPS14 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P62263

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Drosophila melanogaster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Turkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab174661 was affinity purified using an epitope specific to RPS14 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ribosomal Protein S14 (RPS14) also known as 40S ribosomal protein S14 plays an important mechanical role in ribosome assembly. This protein is a component of the small 40S subunit of the ribosome and has a molecular mass of approximately 17 kDa. RPS14 is expressed in various tissues reflecting its importance in general protein synthesis. Its expression in these tissues ensures that the ribosomes can function efficiently to translate mRNA into proteins a necessary process for cellular function and growth.
Biological function summary

RPS14 contributes to ribosome biogenesis and is essential for proper ribosome function. It is part of the ribosomal complex and interacts with ribosomal RNA (rRNA) to help assemble the small subunit of the ribosome. This ensures that the ribosome can accurately process genetic information into polypeptides. The protein's biological role reflects its contribution to maintaining cellular homeostasis and supporting cellular growth through effective protein production.

Pathways

RPS14 has roles in pathways that are important for protein synthesis and cell cycle progression. It plays a part in the ribosome biogenesis pathway which is related to the production and processing of rRNA and works with other ribosomal proteins like RPS19. Additionally it is involved in the p53 signaling pathway where it interacts with proteins that regulate the cell cycle and apoptosis emphasizing its importance in cellular response to stress or damage.

Alterations in RPS14 are linked to specific blood conditions such as myelodysplastic syndromes especially those with deletion of chromosome 5q (5q- syndrome). The protein's alteration affects ribosome function and subsequently protein synthesis. This connection to 5q- syndrome involves interactions with other proteins such as RPS19 which also plays a role in ribosomal activities. Understanding RPS14’s impact on diseases aids in exploring therapeutic targets for related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).
See full target information RPS14
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 51:4208-4222 PubMed37070189

2023

Selective translational control of cellular and viral mRNAs by RPS3 mRNA binding.

Applications

Unspecified application

Species

Unspecified reactive species

Tal Havkin-Solomon,Elad Itzhaki,Nir Joffe,Nina Reuven,Yosef Shaul,Rivka Dikstein

Cell reports 29:3605-3619.e10 PubMed31825839

2019

On-Site Ribosome Remodeling by Locally Synthesized Ribosomal Proteins in Axons.

Applications

Unspecified application

Species

Unspecified reactive species

Toshiaki Shigeoka,Max Koppers,Hovy Ho-Wai Wong,Julie Qiaojin Lin,Roberta Cagnetta,Asha Dwivedy,Janaina de Freitas Nascimento,Francesca W van Tartwijk,Florian Ströhl,Jean-Michel Cioni,Julia Schaeffer,Mark Carrington,Clemens F Kaminski,Hosung Jung,William A Harris,Christine E Holt

eLife 8: PubMed31746735

2019

Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons.

Applications

Unspecified application

Species

Unspecified reactive species

Max Koppers,Roberta Cagnetta,Toshiaki Shigeoka,Lucia Cs Wunderlich,Pedro Vallejo-Ramirez,Julie Qiaojin Lin,Sixian Zhao,Maximilian Ah Jakobs,Asha Dwivedy,Michael S Minett,Anaïs Bellon,Clemens F Kaminski,William A Harris,John G Flanagan,Christine E Holt

Oncology letters 14:7332-7338 PubMed29344171

2018

DEPDC7 inhibits cell proliferation, migration and invasion in hepatoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhijun Liao,Xinrui Wang,Xiaojiang Wang,Lisheng Li,Dexin Lin
View all publications
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