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AB246916

Anti-RPS14 antibody

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(2 Publications)

Rabbit Polyclonal RPS14 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RPS14 aa 1-150.

View Alternative Names

PRO2640, RPS14, Small ribosomal subunit protein uS11, 40S ribosomal protein S14

7 Images
Immunocytochemistry/ Immunofluorescence - Anti-RPS14 antibody (AB246916)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RPS14 antibody (AB246916)

PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling RPS14 using ab246916 at 4 μg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS14 antibody (AB246916)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS14 antibody (AB246916)

Paraffin-embedded human cerebral cortex tissue stained for RPS14 using ab246916 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS14 antibody (AB246916)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS14 antibody (AB246916)

Paraffin-embedded human skin tissue stained for RPS14 using ab246916 at 1/50 dilution in immunohistochemical analysis.

Western blot - Anti-RPS14 antibody (AB246916)
  • WB

Unknown

Western blot - Anti-RPS14 antibody (AB246916)

Lane 1 : Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2 : Human cell line RT-4

All lanes:

Western blot - Anti-RPS14 antibody (ab246916) at 0.4 µg/mL

All lanes:

RT4 (Human urinary bladder cancer cell line) cell lysate

Predicted band size: 16 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS14 antibody (AB246916)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS14 antibody (AB246916)

Paraffin-embedded human fallopian tube tissue stained for RPS14 using ab246916 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS14 antibody (AB246916)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS14 antibody (AB246916)

Paraffin-embedded human colon tissue stained for RPS14 using ab246916 at 1/50 dilution in immunohistochemical analysis.

Western blot - Anti-RPS14 antibody (AB246916)
  • WB

Unknown

Western blot - Anti-RPS14 antibody (AB246916)

All lanes:

Western blot - Anti-RPS14 antibody (ab246916) at 0.4 µg/mL

All lanes:

Mouse cerebral cortex tissue lysate

Predicted band size: 16 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human RPS14 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

P62263

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ribosomal Protein S14 (RPS14) also known as 40S ribosomal protein S14 plays an important mechanical role in ribosome assembly. This protein is a component of the small 40S subunit of the ribosome and has a molecular mass of approximately 17 kDa. RPS14 is expressed in various tissues reflecting its importance in general protein synthesis. Its expression in these tissues ensures that the ribosomes can function efficiently to translate mRNA into proteins a necessary process for cellular function and growth.
Biological function summary

RPS14 contributes to ribosome biogenesis and is essential for proper ribosome function. It is part of the ribosomal complex and interacts with ribosomal RNA (rRNA) to help assemble the small subunit of the ribosome. This ensures that the ribosome can accurately process genetic information into polypeptides. The protein's biological role reflects its contribution to maintaining cellular homeostasis and supporting cellular growth through effective protein production.

Pathways

RPS14 has roles in pathways that are important for protein synthesis and cell cycle progression. It plays a part in the ribosome biogenesis pathway which is related to the production and processing of rRNA and works with other ribosomal proteins like RPS19. Additionally it is involved in the p53 signaling pathway where it interacts with proteins that regulate the cell cycle and apoptosis emphasizing its importance in cellular response to stress or damage.

Alterations in RPS14 are linked to specific blood conditions such as myelodysplastic syndromes especially those with deletion of chromosome 5q (5q- syndrome). The protein's alteration affects ribosome function and subsequently protein synthesis. This connection to 5q- syndrome involves interactions with other proteins such as RPS19 which also plays a role in ribosomal activities. Understanding RPS14’s impact on diseases aids in exploring therapeutic targets for related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).
See full target information RPS14
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell proliferation 56:e13458 PubMed36977657

2023

Rps14 upregulation promotes inner ear progenitor proliferation and hair cell regeneration in the neonatal mouse cochlea.

Applications

Unspecified application

Species

Unspecified reactive species

Changling Xu,Jieyu Qi,Xiaojie Hu,Liyan Zhang,Qiuhan Sun,Nianci Li,Xin Chen,Fangfang Guo,Peina Wu,Yi Shi,Renjie Chai

Frontiers in cell and developmental biology 9:618899 PubMed33644057

2021

Cisplatin-Resistant Gastric Cancer Cells Promote the Chemoresistance of Cisplatin-Sensitive Cells via the Exosomal RPS3-Mediated PI3K-Akt-Cofilin-1 Signaling Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Yao Sun,Bo Xu,Qiu-Xue Wu,Wen-Lian Chen,Si Cai,Hui Zhang,Qing-Feng Tang
View all publications
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