Rabbit Polyclonal RPS14 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RPS14 aa 1-150.
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Publications
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- Cell proliferation 56:e134582023Rps14 upregulation promotes inner ear progenitor proliferation and hair cell regeneration in the neonatal mouse cochlea.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChangling Xu,Jieyu Qi,Xiaojie Hu,Liyan Zhang,Qiuhan Sun,Nianci Li,Xin Chen,Fangfang Guo,Peina Wu,Yi Shi,Renjie ChaiPubMed 36977657
- Frontiers in cell and developmental biology 9:6188992021Cisplatin-Resistant Gastric Cancer Cells Promote the Chemoresistance of Cisplatin-Sensitive Cells via the Exosomal RPS3-Mediated PI3K-Akt-Cofilin-1 Signaling Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeng-Yao Sun,Bo Xu,Qiu-Xue Wu,Wen-Lian Chen,Si Cai,Hui Zhang,Qing-Feng TangPubMed 33644057
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human RPS14 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link P62263
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Alternative names
40S ribosomal protein S14, Small ribosomal subunit protein uS11, RPS14, PRO2640
Recommended products
Rabbit Polyclonal RPS14 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RPS14 aa 1-150.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human RPS14 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link P62263
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Ribosomal Protein S14 (RPS14) also known as 40S ribosomal protein S14 plays an important mechanical role in ribosome assembly. This protein is a component of the small 40S subunit of the ribosome and has a molecular mass of approximately 17 kDa. RPS14 is expressed in various tissues reflecting its importance in general protein synthesis. Its expression in these tissues ensures that the ribosomes can function efficiently to translate mRNA into proteins a necessary process for cellular function and growth.
- Biological function summary
RPS14 contributes to ribosome biogenesis and is essential for proper ribosome function. It is part of the ribosomal complex and interacts with ribosomal RNA (rRNA) to help assemble the small subunit of the ribosome. This ensures that the ribosome can accurately process genetic information into polypeptides. The protein's biological role reflects its contribution to maintaining cellular homeostasis and supporting cellular growth through effective protein production.
- Pathways
RPS14 has roles in pathways that are important for protein synthesis and cell cycle progression. It plays a part in the ribosome biogenesis pathway which is related to the production and processing of rRNA and works with other ribosomal proteins like RPS19. Additionally it is involved in the p53 signaling pathway where it interacts with proteins that regulate the cell cycle and apoptosis emphasizing its importance in cellular response to stress or damage.
- Associated diseases and disorders
Alterations in RPS14 are linked to specific blood conditions such as myelodysplastic syndromes especially those with deletion of chromosome 5q (5q- syndrome). The protein's alteration affects ribosome function and subsequently protein synthesis. This connection to 5q- syndrome involves interactions with other proteins such as RPS19 which also plays a role in ribosomal activities. Understanding RPS14’s impact on diseases aids in exploring therapeutic targets for related disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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7 product images
Immunocytochemistry/ Immunofluorescence - Anti-RPS14 antibody (ab246916)
PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling RPS14 using ab246916 at 4 μg/ml (green) in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS14 antibody (ab246916)
Paraffin-embedded human skin tissue stained for RPS14 using ab246916 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS14 antibody (ab246916)
Paraffin-embedded human colon tissue stained for RPS14 using ab246916 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS14 antibody (ab246916)
Paraffin-embedded human cerebral cortex tissue stained for RPS14 using ab246916 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS14 antibody (ab246916)
Paraffin-embedded human fallopian tube tissue stained for RPS14 using ab246916 at 1/50 dilution in immunohistochemical analysis.
Western blot - Anti-RPS14 antibody (ab246916)
All lanes: Western blot - Anti-RPS14 antibody (ab246916) at 0.4 µg/mL
All lanes: Mouse cerebral cortex tissue lysate
Predicted band size: 16 kDa
Western blot - Anti-RPS14 antibody (ab246916)
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: Human cell line RT-4All lanes: Western blot - Anti-RPS14 antibody (ab246916) at 0.4 µg/mL
All lanes: RT4 (Human urinary bladder cancer cell line) cell lysate
Predicted band size: 16 kDa
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