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AB249457

Anti-RPS15 antibody [EPR11105] - BSA and Azide free

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Rabbit Recombinant Monoclonal RPS15 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.

View Alternative Names

RIG, RPS15, Small ribosomal subunit protein uS19, 40S ribosomal protein S15, RIG protein

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-RPS15 antibody [EPR11105] - BSA and Azide free (AB249457)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RPS15 antibody [EPR11105] - BSA and Azide free (AB249457)

This data was developed using ab168361, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HepG2 cells labeling RPS15 with ab168361 at 1/100 dilution.

Western blot - Anti-RPS15 antibody [EPR11105] - BSA and Azide free (AB249457)
  • WB

Unknown

Western blot - Anti-RPS15 antibody [EPR11105] - BSA and Azide free (AB249457)

This data was developed using ab168361, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RPS15 antibody [EPR11105] (<a href='/en-us/products/primary-antibodies/rps15-antibody-epr11105-ab168361'>ab168361</a>) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

A549 cell lysate at 10 µg

Lane 4:

U-87 MG cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 17 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11105

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab249457 is the carrier-free version of ab168361.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPS15 also known as Ribosomal Protein S15 is a protein that plays an important role in ribosome function. It is a component of the small 40S ribosomal subunit involved in the assembly and stability of ribosomes. The molecular mass of RPS15 is approximately 17 kDa. The protein is expressed in various tissues throughout the body with higher expression levels in actively dividing cells. Its expression is detected in both the cytoplasmic and nuclear compartments indicating its involvement in ribosome biogenesis and protein synthesis.
Biological function summary

The function of RPS15 is important for proper protein synthesis since it is a part of the ribosomal protein complex. It interacts directly with ribosomal RNA and aids in decoding messenger RNA during translation. The protein contributes to maintaining the structure and function of the ribosomal unit. Moreover RPS15 associates with other ribosomal proteins to ensure efficient protein translation especially during cell growth and division.

Pathways

RPS15 participates in pathways associated with ribosome biogenesis and protein synthesis. It plays a role in the translation initiation pathway where it interacts with various evolutionarily conserved proteins to facilitate mRNA decoding. RPS15 also works in conjunction with other ribosomal proteins like RPS19 to co-regulate translational control. These interactions ensure the fidelity and efficiency of protein synthesis within cells.

Abnormalities in RPS15 expression or function have been linked to cancer development particularly in hematological malignancies such as chronic lymphocytic leukemia. Mutations in RPS15 can disrupt normal ribosome function leading to unchecked cell growth and division. Furthermore RPS15 is associated with other ribosomal proteins like RPS20 in the context of diseases emphasizing the interconnectivity between ribosomal components in pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the small ribosomal subunit (PubMed : 23636399). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399).
See full target information RPS15
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com