Rabbit Recombinant Monoclonal RPS16 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IP | Flow Cyt | WB | IHC-P | ICC/IF | |
---|---|---|---|---|---|
Human | Tested | Not recommended | Tested | Tested | Tested |
Mouse | Expected | Not recommended | Tested | Expected | Expected |
Rat | Expected | Not recommended | Tested | Expected | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 - 1/5000 | Notes - |
Species Mouse | Dilution info 1/1000 - 1/5000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/5000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Component of the small ribosomal subunit (PubMed:23636399). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:23636399). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed:34516797).
Small ribosomal subunit protein uS9, 40S ribosomal protein S16, RPS16
Rabbit Recombinant Monoclonal RPS16 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR11755
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
RPS16 also known as Ribosomal Protein S16 is a component of the 40S ribosomal subunit with a molecular mass of approximately 16.5 kDa. It plays a role in protein synthesis by joining together with other ribosomal proteins and rRNA to form the ribosome's small subunit. RPS16 is expressed in various tissues throughout the human body reflecting its essential role in maintaining protein synthesis machinery.
The function of this ribosomal protein contributes to the translation process by ensuring the proper assembly and stability of the ribosomal small subunit. RPS16 is part of the ribosomal complex consisting of rRNA and numerous ribosomal proteins that collaborate to decode mRNA into amino acid sequences. This protein ensures the accuracy of translation initiation and elongation vital for efficient cellular function and growth.
Ribosomal proteins like RPS16 participate in the translation pathway facilitating mRNA translation and protein synthesis. It plays a significant role in the ribosome biogenesis pathway coordinating with proteins such as RPS18 and RPL5 in the assembly and maturation of ribosomal subunits. These interactions are important for the cellular response to growth signals and metabolic stress.
RPS16 shows an association with Diamond-Blackfan Anemia a rare genetic disorder characterized by a failure to produce enough red blood cells. Mutations in genes encoding ribosomal proteins including RPS16 can disrupt ribosome biogenesis impairing erythropoiesis. This protein's abnormalities can also loosely link to certain cancers as ribosomal dysfunction may lead to uncontrolled cell growth and division through altered expression of proteins like c-Myc which regulates ribosome biogenesis.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Western blot analysis on immunoprecipitation pellet from fetal liver lysate using ab177951.
All lanes: Immunoprecipitation - Anti-RPS16 antibody [EPR11755] (ab177951)
Predicted band size: 16 kDa
Immunofluorescent analysis of MCF-7 cells labeling RPS16 with ab177951 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded breast carcinoma tissue labeling RPS16 with ab177951 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
All lanes: Western blot - Anti-RPS16 antibody [EPR11755] (ab177951) at 1/1000 dilution
Lane 1: MCF-7 cell lysate at 10 µg
Lane 2: Fetal liver lysate at 10 µg
Lane 3: HeLa cell lysate at 10 µg
Lane 4: Ramos cell lysate at 10 µg
Lane 5: PC-12 cell lysate at 10 µg
Lane 6: NIH/3T3 cell lysate at 10 µg
Predicted band size: 16 kDa
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