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Rabbit Recombinant Monoclonal RPS16 antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Immunoprecipitation - Anti-RPS16 antibody [EPR11755] - BSA and Azide free (AB250022), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-RPS16 antibody [EPR11755] - BSA and Azide free (AB250022), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS16 antibody [EPR11755] - BSA and Azide free (AB250022), expandable thumbnail
  • Western blot - Anti-RPS16 antibody [EPR11755] - BSA and Azide free (AB250022), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPFlow CytWBIHC-P
Human
Tested
Tested
Not recommended
Tested
Tested
Mouse
Expected
Expected
Not recommended
Tested
Expected
Rat
Expected
Expected
Not recommended
Tested
Expected

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Component of the small ribosomal subunit (PubMed:23636399). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:23636399). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed:34516797).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal RPS16 antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR11755
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab250022 is the carrier-free version of Anti-RPS16 antibody [EPR11755] ab177951.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

RPS16 also known as Ribosomal Protein S16 is a component of the 40S ribosomal subunit with a molecular mass of approximately 16.5 kDa. It plays a role in protein synthesis by joining together with other ribosomal proteins and rRNA to form the ribosome's small subunit. RPS16 is expressed in various tissues throughout the human body reflecting its essential role in maintaining protein synthesis machinery.

Biological function summary

The function of this ribosomal protein contributes to the translation process by ensuring the proper assembly and stability of the ribosomal small subunit. RPS16 is part of the ribosomal complex consisting of rRNA and numerous ribosomal proteins that collaborate to decode mRNA into amino acid sequences. This protein ensures the accuracy of translation initiation and elongation vital for efficient cellular function and growth.

Pathways

Ribosomal proteins like RPS16 participate in the translation pathway facilitating mRNA translation and protein synthesis. It plays a significant role in the ribosome biogenesis pathway coordinating with proteins such as RPS18 and RPL5 in the assembly and maturation of ribosomal subunits. These interactions are important for the cellular response to growth signals and metabolic stress.

Associated diseases and disorders

RPS16 shows an association with Diamond-Blackfan Anemia a rare genetic disorder characterized by a failure to produce enough red blood cells. Mutations in genes encoding ribosomal proteins including RPS16 can disrupt ribosome biogenesis impairing erythropoiesis. This protein's abnormalities can also loosely link to certain cancers as ribosomal dysfunction may lead to uncontrolled cell growth and division through altered expression of proteins like c-Myc which regulates ribosome biogenesis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunoprecipitation - Anti-RPS16 antibody [EPR11755] - BSA and Azide free (ab250022), expandable thumbnail

    Immunoprecipitation - Anti-RPS16 antibody [EPR11755] - BSA and Azide free (ab250022)

    This data was developed using Anti-RPS16 antibody [EPR11755] ab177951, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from fetal liver lysate using Anti-RPS16 antibody [EPR11755] ab177951.

    All lanes: Immunoprecipitation - Anti-RPS16 antibody [EPR11755] (Anti-RPS16 antibody [EPR11755] ab177951)

    Predicted band size: 16 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-RPS16 antibody [EPR11755] - BSA and Azide free (ab250022), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-RPS16 antibody [EPR11755] - BSA and Azide free (ab250022)

    This data was developed using Anti-RPS16 antibody [EPR11755] ab177951, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of MCF-7 cells labeling RPS16 with Anti-RPS16 antibody [EPR11755] ab177951 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS16 antibody [EPR11755] - BSA and Azide free (ab250022), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS16 antibody [EPR11755] - BSA and Azide free (ab250022)

    This data was developed using Anti-RPS16 antibody [EPR11755] ab177951, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded breast carcinoma tissue labeling RPS16 with Anti-RPS16 antibody [EPR11755] ab177951 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-RPS16 antibody [EPR11755] - BSA and Azide free (ab250022), expandable thumbnail

    Western blot - Anti-RPS16 antibody [EPR11755] - BSA and Azide free (ab250022)

    This data was developed using Anti-RPS16 antibody [EPR11755] ab177951, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-RPS16 antibody [EPR11755] (Anti-RPS16 antibody [EPR11755] ab177951) at 1/1000 dilution

    Lane 1: MCF-7 cell lysate at 10 µg

    Lane 2: Fetal liver lysate at 10 µg

    Lane 3: HeLa cell lysate at 10 µg

    Lane 4: Ramos cell lysate at 10 µg

    Lane 5: PC-12 cell lysate at 10 µg

    Lane 6: NIH/3T3 cell lysate at 10 µg

    Predicted band size: 16 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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