AB250022

Anti-RPS16 antibody [EPR11755] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal RPS16 antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Small ribosomal subunit protein uS9, 40S ribosomal protein S16, RPS16

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-RPS16 antibody [EPR11755] - BSA and Azide free (AB250022)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RPS16 antibody [EPR11755] - BSA and Azide free (AB250022)

This data was developed using ab177951, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of MCF-7 cells labeling RPS16 with ab177951 at 1/100 dilution.

Supplier Data

Western blot - Anti-RPS16 antibody [EPR11755] - BSA and Azide free (AB250022)

This data was developed using ab177951, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RPS16 antibody [EPR11755] (<a href='/en-us/products/primary-antibodies/rps16-antibody-epr11755-ab177951'>ab177951</a>) at 1/1000 dilution

Lane 1:

MCF-7 cell lysate at 10 µg

Lane 2:

Fetal liver lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

Ramos cell lysate at 10 µg

Lane 5:

PC-12 cell lysate at 10 µg

Lane 6:

NIH/3T3 cell lysate at 10 µg

Predicted band size: 16 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS16 antibody [EPR11755] - BSA and Azide free (AB250022)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS16 antibody [EPR11755] - BSA and Azide free (AB250022)

This data was developed using ab177951, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded breast carcinoma tissue labeling RPS16 with ab177951 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-RPS16 antibody [EPR11755] - BSA and Azide free (AB250022)

This data was developed using ab177951, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from fetal liver lysate using ab177951.

All lanes:

Immunoprecipitation - Anti-RPS16 antibody [EPR11755] (<a href='/en-us/products/primary-antibodies/rps16-antibody-epr11755-ab177951'>ab177951</a>)

Predicted band size: 16 kDa

false

Unconjugated

Anti-RPS16 antibody [EPR11755]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11755

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab250022 is the carrier-free version of ab177951.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPS16 also known as Ribosomal Protein S16 is a component of the 40S ribosomal subunit with a molecular mass of approximately 16.5 kDa. It plays a role in protein synthesis by joining together with other ribosomal proteins and rRNA to form the ribosome's small subunit. RPS16 is expressed in various tissues throughout the human body reflecting its essential role in maintaining protein synthesis machinery.

Biological function summary

The function of this ribosomal protein contributes to the translation process by ensuring the proper assembly and stability of the ribosomal small subunit. RPS16 is part of the ribosomal complex consisting of rRNA and numerous ribosomal proteins that collaborate to decode mRNA into amino acid sequences. This protein ensures the accuracy of translation initiation and elongation vital for efficient cellular function and growth.

Pathways

Ribosomal proteins like RPS16 participate in the translation pathway facilitating mRNA translation and protein synthesis. It plays a significant role in the ribosome biogenesis pathway coordinating with proteins such as RPS18 and RPL5 in the assembly and maturation of ribosomal subunits. These interactions are important for the cellular response to growth signals and metabolic stress.

RPS16 shows an association with Diamond-Blackfan Anemia a rare genetic disorder characterized by a failure to produce enough red blood cells. Mutations in genes encoding ribosomal proteins including RPS16 can disrupt ribosome biogenesis impairing erythropoiesis. This protein's abnormalities can also loosely link to certain cancers as ribosomal dysfunction may lead to uncontrolled cell growth and division through altered expression of proteins like c-Myc which regulates ribosome biogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the small ribosomal subunit (PubMed : 23636399). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).

See full target information RPS16

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 31:65 PubMed39972431

2025

Mechanism of histone demethylase KDM5A in osteoporotic fracture healing through epigenetic regulation of the miR-495/SKP2/Runx2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuoran Li,Junyan Zhang,Tingting Xu,Zhiying Hao,Yadong Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPS16 antibody [EPR11755] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-RPS16 antibody [EPR11755] - BSA and Azide free (AB250022)

Western blot - Anti-RPS16 antibody [EPR11755] - BSA and Azide free (AB250022)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS16 antibody [EPR11755] - BSA and Azide free (AB250022)

Immunoprecipitation - Anti-RPS16 antibody [EPR11755] - BSA and Azide free (AB250022)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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Product promise