Goat Polyclonal RPS19 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human 40S ribosomal protein S19 aa 100 to C-terminus.
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Publications
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- Life science alliance 6:2023Myc controls NK cell development, IL-15-driven expansion, and translational machinery.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHanif J Khameneh,Nicolas Fonta,Alessandro Zenobi,Charlène Niogret,Pedro Ventura,Concetta Guerra,Ivo Kwee,Andrea Rinaldi,Matteo Pecoraro,Roger Geiger,Andrea Cavalli,Francesco Bertoni,Eric Vivier,Andreas Trumpp,Greta GuardaPubMed 37105715
- Experimental hematology 111:66-782022Downregulation of SATB1 by miRNAs reduces megakaryocyte/erythroid progenitor expansion in preclinical models of Diamond-Blackfan anemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMark C Wilkes,Vanessa Scanlon,Aya Shibuya,Alma-Martina Cepika,Ascia Eskin,Zugen Chen,Anupama Narla,Bert Glader,Maria Grazia Roncarolo,Stanley F Nelson,Kathleen M SakamotoPubMed 35460833
- Blood 124:3791-82014TNF-mediated inflammation represses GATA1 and activates p38 MAP kinase in RPS19-deficient hematopoietic progenitors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElena Bibikova et. al.PubMed 25270909
- Journal of cellular physiology 229:1047-602013Identification of the PKR nuclear interactome reveals roles in ribosome biogenesis, mRNA processing and cell division.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWilliam L Blalock,Manuela Piazzi,Alberto Bavelloni,Mirco Raffini,Irene Faenza,Antonietta D'Angelo,Lucio CoccoPubMed 24347309
- Blood 119:262-722011Ribosomal deficiencies in Diamond-Blackfan anemia impair translation of transcripts essential for differentiation of murine and human erythroblasts.Applications:WBReactive species:MouseRastislav Horos,Hanna Ijspeert,Dagmar Pospisilova,Regine Sendtner,Charlotte Andrieu-Soler,Erdogan Taskesen,Andrzej Nieradka,Radek Cmejla,Michael Sendtner,Ivo P Touw,Marieke von LindernPubMed 22058113
- Immunity 32:329-412010Delivery of cytosolic components by autophagic adaptor protein p62 endows autophagosomes with unique antimicrobial properties.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human 40S ribosomal protein S19 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P39019
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cat | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Cat, Dog, Chimpanzee, Rhesus monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.10000-0.30000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Cat, Dog, Chimpanzee, Rhesus monkey | Dilution info - | Notes - |
Associated Products
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Target data
Function
Component of the small ribosomal subunit (PubMed:23636399). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:23636399). Required for pre-rRNA processing and maturation of 40S ribosomal subunits (PubMed:16990592). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed:34516797).
Alternative names
Small ribosomal subunit protein eS19, 40S ribosomal protein S19, RPS19
Recommended products
Goat Polyclonal RPS19 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human 40S ribosomal protein S19 aa 100 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human 40S ribosomal protein S19 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P39019
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RPS19 also known as Ribosomal Protein S19 is a component of the small 40S ribosomal subunit. It has a molecular weight of approximately 16 kDa. This protein is widely expressed in many tissues including the bone marrow and spleen which are important for hematopoiesis. RPS19 plays a mechanical role in ensuring the formation and function of ribosomes which are essential for protein synthesis. By participating in ribosome assembly it supports the translational machinery of cells.
- Biological function summary
Ribosomal protein S19 holds a critical place in ribosome biogenesis. It is part of the ribosomal protein family and works together with other ribosomal proteins to create functional ribosomes necessary for cellular protein synthesis. As part of the 40S ribosomal subunit RPS19 facilitates the accurate translation of mRNA into proteins which is fundamental for cell growth and proliferation. The 40S subunit alongside the 60S subunit completes the formation of the functional ribosome.
- Pathways
Ribosomal protein S19 contributes to the essential process of translation an important pathway within cell biology. It plays a part in the ribosome assembly pathway a pivotal process necessary for cell survival and function. RPS19 interacts closely with other ribosomal proteins such as RPS24 and RPS14 ensuring the proper assembly and functionality of the ribosomal subunits. These interactions are integral to maintaining cellular homeostasis and protein production.
- Associated diseases and disorders
Ribosomal protein S19 is associated with Diamond-Blackfan Anemia a rare genetic anemia caused by ribosomal protein mutations. This condition arises due to defective ribosome biogenesis leading to impaired erythropoiesis. Mutations in RPS19 reduce the ribosome's capacity to synthesize proteins specifically affecting red blood cell production. Additionally the protein is related to other ribosomal anomalies that connect to various bone marrow failure syndromes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-RPS19 antibody (ab40833)
All lanes: Western blot - Anti-RPS19 antibody (ab40833) at 0.1 µg/mL
All lanes: K562 cell lysate in RIPA buffer at 35 µg
Predicted band size: 16 kDa
Observed band size: 15 kDa
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