AB137889

Anti-RPS2 antibody

Name: Anti-RPS2 antibody (ab137889) | Abcam
Brand: Abcam Limited
SKU: AB137889
Price: 535 USD
Availability: InStock

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Rabbit Polyclonal RPS2 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human RPS2 aa 50-100.

View Alternative Names

RPS4, RPS2, Small ribosomal subunit protein uS5, 40S ribosomal protein S2, 40S ribosomal protein S4, Protein LLRep3

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Immunoprecipitation - Anti-RPS2 antibody (AB137889)

ab137889 at 1µg/ml staining RPS2 by western blot, in 293T whole cell lysate immunoprecipitated using ab137889 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

All lanes:

Immunoprecipitation - Anti-RPS2 antibody (ab137889)

Predicted band size: 31 kDa

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Unknown

Western blot - Anti-RPS2 antibody (AB137889)

All lanes:

Western blot - Anti-RPS2 antibody (ab137889) at 1/2000 dilution

Lane 1:

HeLa cell lysate at 50 µg

Lane 2:

HeLa cell lysate at 15 µg

Lane 3:

293T cell lysate at 50 µg

Lane 4:

Jurkat cell lysate at 50 µg

Predicted band size: 31 kDa

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Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human RPS2 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

P15880

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Snake": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99.01% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPS2 also known as Ribosomal Protein S2 is a component of the 40S subunit of the ribosome. This protein plays a mechanical role in ribosome assembly and protein synthesis. RPS2 has a molecular mass of approximately 29 kDa. Researchers have observed its expression in various tissues indicating its involvement in general cellular processes. It facilitates the initiation of translation by binding to ribosomal RNA and other ribosomal proteins to form a functional ribosome.

Biological function summary

Ribosomal Protein S2 contributes significantly to ribosome function and protein translation. As part of the ribosome RPS2 aids in the decoding of messenger RNA (mRNA) into proteins which occurs in all cellular environments. This protein works in conjunction with other ribosomal proteins and rRNA to ensure accuracy and efficiency in protein synthesis. It forms an integral part of the ribosomal machinery that also includes many other proteins and RNA molecules congregating to form the translation initiation complex.

Pathways

Several biological systems depend on the role that Ribosomal Protein S2 plays in protein synthesis. The mTOR signaling pathway is particularly important as RPS2 and other ribosomal proteins function under the regulation of mTOR to control protein translation rates. Additionally RPS2 connects with proteins like eIF2 to influence translation initiation. Together they regulate critical processes such as cell growth and proliferation linking ribosomal function with cellular metabolic status.

Impaired Ribosomal Protein S2 function can lead to conditions such as Diamond-Blackfan anemia which results from defects in ribosome biogenesis. Mutations or disruptions in related proteins like RPS19 are often studied alongside RPS2 for their roles in hereditary and acquired anemias. Furthermore aberrant RPS2 expression can contribute to tumorigenesis implicating it in cancer studies where proteins like p53 may interact or affect its function revealing the broader implications of ribosomal proteins in human health and diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399). The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules (PubMed : 23636399). The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain (PubMed : 23636399). The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel (PubMed : 23636399). Plays a role in the assembly and function of the 40S ribosomal subunit (By similarity). Mutations in this protein affects the control of translational fidelity (By similarity). Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly (By similarity).

See full target information RPS2

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-RPS2 antibody

Immunoprecipitation - Anti-RPS2 antibody (AB137889)

Western blot - Anti-RPS2 antibody (AB137889)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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