Rabbit Recombinant Monoclonal RPS2 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
IHC-P | ICC/IF | IP | Flow Cyt | WB | |
---|---|---|---|---|---|
Human | Tested | Tested | Not recommended | Not recommended | Tested |
Mouse | Predicted | Predicted | Not recommended | Not recommended | Predicted |
Rat | Predicted | Predicted | Not recommended | Not recommended | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Use of a HRP/AP polymerized secondary antibody is recommended. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Select an associated product type
40S ribosomal protein S2, 40S ribosomal protein S4, Protein LLRep3, Small ribosomal subunit protein uS5, RPS4, RPS2
Rabbit Recombinant Monoclonal RPS2 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.
40S ribosomal protein S2, 40S ribosomal protein S4, Protein LLRep3, Small ribosomal subunit protein uS5, RPS4, RPS2
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Yes
EPR10834(B)
Affinity purification Protein A
Blue Ice
+4°C
Do Not Freeze
ab249215 is the carrier-free version of Anti-RPS2 antibody [EPR10834(B)] ab155961.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
RPS2 also known as Ribosomal Protein S2 is a component of the 40S subunit of the ribosome. This protein plays a mechanical role in ribosome assembly and protein synthesis. RPS2 has a molecular mass of approximately 29 kDa. Researchers have observed its expression in various tissues indicating its involvement in general cellular processes. It facilitates the initiation of translation by binding to ribosomal RNA and other ribosomal proteins to form a functional ribosome.
Ribosomal Protein S2 contributes significantly to ribosome function and protein translation. As part of the ribosome RPS2 aids in the decoding of messenger RNA (mRNA) into proteins which occurs in all cellular environments. This protein works in conjunction with other ribosomal proteins and rRNA to ensure accuracy and efficiency in protein synthesis. It forms an integral part of the ribosomal machinery that also includes many other proteins and RNA molecules congregating to form the translation initiation complex.
Several biological systems depend on the role that Ribosomal Protein S2 plays in protein synthesis. The mTOR signaling pathway is particularly important as RPS2 and other ribosomal proteins function under the regulation of mTOR to control protein translation rates. Additionally RPS2 connects with proteins like eIF2 to influence translation initiation. Together they regulate critical processes such as cell growth and proliferation linking ribosomal function with cellular metabolic status.
Impaired Ribosomal Protein S2 function can lead to conditions such as Diamond-Blackfan anemia which results from defects in ribosome biogenesis. Mutations or disruptions in related proteins like RPS19 are often studied alongside RPS2 for their roles in hereditary and acquired anemias. Furthermore aberrant RPS2 expression can contribute to tumorigenesis implicating it in cancer studies where proteins like p53 may interact or affect its function revealing the broader implications of ribosomal proteins in human health and diseases.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
This data was developed using Anti-RPS2 antibody [EPR10834(B)] ab155961, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling RPS2 with Anti-RPS2 antibody [EPR10834(B)] ab155961 at a 1/1000 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using Anti-RPS2 antibody [EPR10834(B)] ab155961, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-RPS2 antibody [EPR10834(B)] (Anti-RPS2 antibody [EPR10834(B)] ab155961) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: A549 cell lysate at 10 µg
Lane 3: 293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg
All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 31 kDa
This data was developed using Anti-RPS2 antibody [EPR10834(B)] ab155961, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling RPS2 with Anti-RPS2 antibody [EPR10834(B)] ab155961 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using Anti-RPS2 antibody [EPR10834(B)] ab155961, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of HeLa cells labeling RPS2 with Anti-RPS2 antibody [EPR10834(B)] ab155961 at a 1/100 dilution.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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