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AB133776

Anti-RPS20 antibody [EPR8716]

4

(2 Reviews)

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(18 Publications)

Rabbit Recombinant Monoclonal RPS20 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 18 publications.

View Alternative Names

Small ribosomal subunit protein uS10, 40S ribosomal protein S20, RPS20

4 Images
Flow Cytometry (Intracellular) - Anti-RPS20 antibody [EPR8716] (AB133776)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-RPS20 antibody [EPR8716] (AB133776)

Intracellular Flow Cytometry analysis of A431cells (human epidermoid carcinoma) labeling RPS20 with purified ab133776 at 1/250 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) (ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS20 antibody [EPR8716] (AB133776)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS20 antibody [EPR8716] (AB133776)

Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue labelling RPS20 using ab133776 at 1/100 dilution

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-RPS20 antibody [EPR8716] (AB133776)
  • WB

Unknown

Western blot - Anti-RPS20 antibody [EPR8716] (AB133776)

All lanes:

Western blot - Anti-RPS20 antibody [EPR8716] (ab133776) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

A431 cell lysate at 10 µg

Lane 3:

HUVEC cell lysate at 10 µg

Lane 4:

Human placenta cell lysate at 10 µg

Secondary

All lanes:

HRP-labelled Goat anti-Rabbit at 1/2000 dilution

Predicted band size: 13 kDa

false

OI-RD Scanning - Anti-RPS20 antibody [EPR8716] (AB133776)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-RPS20 antibody [EPR8716] (AB133776)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8716

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPS20 also known as Ribosomal Protein S20 is a component of the ribosome with a molecular weight of approximately 13 kDa. It operates within the small 40S subunit of the ribosome and plays an important role in protein synthesis by facilitating translation. RPS20 shows expression mainly in the cytosol and nucleus of cells where ribosome assembly and function occur.
Biological function summary

This ribosomal protein contributes to accurate translation and assists in the binding of tRNA and mRNA to the ribosome. RPS20 is an essential part of the ribosomal complex that influences the fidelity and efficiency of protein synthesis. Any alterations in ribosomal protein components like RPS20 can lead to downstream effects on protein production impacting cellular processes such as growth and differentiation.

Pathways

The activity of RPS20 is associated with the ribosome biogenesis and translation pathways which are central to cellular protein synthesis. It closely interacts with other ribosomal proteins such as RPS19 and RPS21 within these pathways to ensure proper ribosome assembly and function. This interplay maintains the balance of protein synthesis influencing cellular metabolism and overall homeostasis.

Mutations or dysregulation in RPS20 are linked to certain forms of ribosomopathies such as Diamond-Blackfan anemia. This disorder is characterized by impaired red blood cell production where ribosomal protein mutations disrupt normal erythropoiesis. RPS20's connection to ribosomopathies also relates to other ribosomal proteins like RPL11 which share involvement in similar pathways and disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the small ribosomal subunit (PubMed : 23636399). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399).
See full target information RPS20
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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Nature structural & molecular biology 32:1932-1946 PubMed40646310

2025

Comparative CRISPRi screens reveal a human stem cell dependence on mRNA translation-coupled quality control.

Applications

Unspecified application

Species

Unspecified reactive species

Geraldine Rodschinka,Sergio Forcelloni,Felix M Kühner,Sascha Wani,Henrick Riemenschneider,Dieter Edbauer,Andrew Behrens,Danny D Nedialkova

Autophagy 21:996-1018 PubMed39842454

2025

The Epstein-Barr virus deubiquitinase BPLF1 regulates stress-induced ribosome UFMylation and reticulophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Jiangnan Liu,Noemi Nagy,Carlos Ayala-Torres,Solenne Bleuse,Francisco Aguilar-Alonso,Ola Larsson,Maria G Masucci

Nature communications 15:10350 PubMed39609413

2024

E3 ubiquitin ligase RNF10 promotes dissociation of stalled ribosomes and responds to ribosomal subunit imbalance.

Applications

Unspecified application

Species

Unspecified reactive species

Janina A Lehmann,Doris Lindner,Hsu-Min Sung,Georg Stoecklin

Nature 633:718-724 PubMed39169182

2024

NAC guides a ribosomal multienzyme complex for nascent protein processing.

Applications

Unspecified application

Species

Unspecified reactive species

Alfred M Lentzsch,Denis Yudin,Martin Gamerdinger,Sowmya Chandrasekar,Laurenz Rabl,Alain Scaiola,Elke Deuerling,Nenad Ban,Shu-Ou Shan

Molecular & cellular proteomics : MCP 22:100548 PubMed37059365

2023

Ubiquitinome Profiling Reveals in Vivo UBE2D3 Targets and Implicates UBE2D3 in Protein Quality Control.

Applications

Unspecified application

Species

Unspecified reactive species

Zeliha Yalçin,Daniëlle Koot,Karel Bezstarosti,Daniel Salas-Lloret,Onno B Bleijerveld,Vera Boersma,Mattia Falcone,Román González-Prieto,Maarten Altelaar,Jeroen A A Demmers,Jacqueline J L Jacobs

RNA biology 19:437-452 PubMed35388737

2022

Preferential translation of p53 target genes.

Applications

Unspecified application

Species

Unspecified reactive species

Miharu Hisaoka,Johanna Schott,Toman Bortecen,Doris Lindner,Jeroen Krijgsveld,Georg Stoecklin

EMBO reports 22:e52170 PubMed34605609

2021

Muscle-specific Cand2 is translationally upregulated by mTORC1 and promotes adverse cardiac remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Agnieszka A Górska,Clara Sandmann,Eva Riechert,Christoph Hofmann,Ellen Malovrh,Eshita Varma,Vivien Kmietczyk,Julie Ölschläger,Lonny Jürgensen,Verena Kamuf-Schenk,Claudia Stroh,Jennifer Furkel,Mathias H Konstandin,Carsten Sticht,Etienne Boileau,Christoph Dieterich,Norbert Frey,Hugo A Katus,Shirin Doroudgar,Mirko Völkers

Journal of cell science 134: PubMed33912921

2021

Ribosome quality control activity potentiates vaccinia virus protein synthesis during infection.

Applications

Unspecified application

Species

Unspecified reactive species

Elayanambi Sundaramoorthy,Andrew P Ryan,Amit Fulzele,Marilyn Leonard,Matthew D Daugherty,Eric J Bennett

Frontiers in cell and developmental biology 9:618899 PubMed33644057

2021

Cisplatin-Resistant Gastric Cancer Cells Promote the Chemoresistance of Cisplatin-Sensitive Cells via the Exosomal RPS3-Mediated PI3K-Akt-Cofilin-1 Signaling Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Yao Sun,Bo Xu,Qiu-Xue Wu,Wen-Lian Chen,Si Cai,Hui Zhang,Qing-Feng Tang

Human mutation 41:1918-1930 PubMed32790018

2020

Expansion of germline RPS20 mutation phenotype to include Diamond-Blackfan anemia.

Applications

Unspecified application

Species

Unspecified reactive species

Saleh Bhar,Fujun Zhou,Lucas C Reineke,Danna K Morris,Payal P Khincha,Neelam Giri,Lisa Mirabello,Katie Bergstrom,Laramie D Lemon,Christopher L Williams,Yukimatsu Toh,M Tarek Elghetany,Richard E Lloyd,Blanche P Alter,Sharon A Savage,Alison A Bertuch
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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