Rabbit Recombinant Monoclonal RPS20 antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Images
![Flow Cytometry (Intracellular) - Anti-RPS20 antibody [EPR8716] - BSA and Azide free (AB240081), expandable thumbnail](https://content.abcam.com/products/images/rps20-antibody-epr8716-bsa-and-azide-free-ab240081--flow-cytometry-intracellular-img50083.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-RPS20 antibody [EPR8716] - BSA and Azide free (AB240081)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS20 antibody [EPR8716] - BSA and Azide free (AB240081), expandable thumbnail](https://content.abcam.com/products/images/rps20-antibody-epr8716-bsa-and-azide-free-ab240081--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img126448.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS20 antibody [EPR8716] - BSA and Azide free (AB240081)")
![OI-RD Scanning - Anti-RPS20 antibody [EPR8716] - BSA and Azide free (AB240081), expandable thumbnail](https://content.abcam.com/products/images/rps20-antibody-epr8716-bsa-and-azide-free-ab240081--oi-rd-scanning-img97699.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-RPS20 antibody [EPR8716] - BSA and Azide free (AB240081)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Expected | Tested |
| Mouse | Predicted | Not recommended | Expected | Predicted |
| Rat | Predicted | Not recommended | Expected | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Version
Target data
Function
Component of the small ribosomal subunit (PubMed:23636399). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:23636399).
Alternative names
Small ribosomal subunit protein uS10, 40S ribosomal protein S20, RPS20
Recommended products
Rabbit Recombinant Monoclonal RPS20 antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR8716
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 2.51 x 10-10 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab240081 is the carrier-free version of Anti-RPS20 antibody [EPR8716] ab133776.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RPS20 also known as Ribosomal Protein S20 is a component of the ribosome with a molecular weight of approximately 13 kDa. It operates within the small 40S subunit of the ribosome and plays an important role in protein synthesis by facilitating translation. RPS20 shows expression mainly in the cytosol and nucleus of cells where ribosome assembly and function occur.
- Biological function summary
This ribosomal protein contributes to accurate translation and assists in the binding of tRNA and mRNA to the ribosome. RPS20 is an essential part of the ribosomal complex that influences the fidelity and efficiency of protein synthesis. Any alterations in ribosomal protein components like RPS20 can lead to downstream effects on protein production impacting cellular processes such as growth and differentiation.
- Pathways
The activity of RPS20 is associated with the ribosome biogenesis and translation pathways which are central to cellular protein synthesis. It closely interacts with other ribosomal proteins such as RPS19 and RPS21 within these pathways to ensure proper ribosome assembly and function. This interplay maintains the balance of protein synthesis influencing cellular metabolism and overall homeostasis.
- Associated diseases and disorders
Mutations or dysregulation in RPS20 are linked to certain forms of ribosomopathies such as Diamond-Blackfan anemia. This disorder is characterized by impaired red blood cell production where ribosomal protein mutations disrupt normal erythropoiesis. RPS20's connection to ribosomopathies also relates to other ribosomal proteins like RPL11 which share involvement in similar pathways and disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Flow Cytometry (Intracellular) - Anti-RPS20 antibody [EPR8716] - BSA and Azide free (ab240081), expandable thumbnail](https://content.abcam.com/products/images/rps20-antibody-epr8716-bsa-and-azide-free-ab240081--flow-cytometry-intracellular-img50083.jpg "Flow Cytometry (Intracellular) - Anti-RPS20 antibody [EPR8716] - BSA and Azide free (ab240081)")
Flow Cytometry (Intracellular) - Anti-RPS20 antibody [EPR8716] - BSA and Azide free (ab240081)
Intracellular Flow Cytometry analysis of A431cells (human epidermoid carcinoma) labeling RPS20 with purified Anti-RPS20 antibody [EPR8716] ab133776 at 1/250 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RPS20 antibody [EPR8716] ab133776).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS20 antibody [EPR8716] - BSA and Azide free (ab240081), expandable thumbnail](https://content.abcam.com/products/images/rps20-antibody-epr8716-bsa-and-azide-free-ab240081--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img126448.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS20 antibody [EPR8716] - BSA and Azide free (ab240081)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS20 antibody [EPR8716] - BSA and Azide free (ab240081)
Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue labelling RPS20 using Anti-RPS20 antibody [EPR8716] ab133776 at 1/100 dilution
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RPS20 antibody [EPR8716] ab133776).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![OI-RD Scanning - Anti-RPS20 antibody [EPR8716] - BSA and Azide free (ab240081), expandable thumbnail](https://content.abcam.com/products/images/rps20-antibody-epr8716-bsa-and-azide-free-ab240081--oi-rd-scanning-img97699.jpg "OI-RD Scanning - Anti-RPS20 antibody [EPR8716] - BSA and Azide free (ab240081)")
OI-RD Scanning - Anti-RPS20 antibody [EPR8716] - BSA and Azide free (ab240081)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
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