AB254670

Anti-RPS21 antibody

Name: Anti-RPS21 antibody (ab254670) | Abcam
Brand: Abcam Limited
SKU: AB254670

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(1 Publication)

Rabbit Polyclonal RPS21 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RPS21 aa 1 to C-terminus.

View Alternative Names

Small ribosomal subunit protein eS21, 40S ribosomal protein S21, RPS21

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS21 antibody (AB254670)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS21 antibody (AB254670)

Paraffin-embedded human skin tissue stained for RPS21 using ab254670 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS21 antibody (AB254670)

Paraffin-embedded human gastrointestinal tissue stained for RPS21 using ab254670 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS21 antibody (AB254670)

Paraffin-embedded human lymph node tissue stained for RPS21 using ab254670 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS21 antibody (AB254670)

Paraffin-embedded human fallopian tube tissue stained for RPS21 using ab254670 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-RPS21 antibody (AB254670)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RPS21 antibody (AB254670)

PFA fixed, Triton X-100 permeabilized U-251 MG (Human brain glioma cell line) cells labeling RPS21 using ab254670 at 4µg/ml (green) in ICC/IF.

Supplier Data

Western blot - Anti-RPS21 antibody (AB254670)

Lane 1:

Western blot - Anti-RPS21 antibody (ab254670)

Lanes 2 - 3:

Western blot - Anti-RPS21 antibody (ab254670) at 0.4 µg/mL

Lane 1:

DNA Ladder

Lane 2:

NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)

Lane 3:

NBT-II cell lysate (Rat Wistar bladder tumour cells)

Predicted band size: 9 kDa

false

Supplier Data

Western blot - Anti-RPS21 antibody (AB254670)

Lane 1:

Western blot - Anti-RPS21 antibody (ab254670)

Lanes 2 - 3:

Western blot - Anti-RPS21 antibody (ab254670) at 0.4 µg/mL

Lane 1:

DNA Ladder

Lane 2:

RT-4 Cells

Lane 3:

U-251MG cells

Predicted band size: 9 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human RPS21 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P63220

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPS21 also known as Ribosomal Protein S21 is a component of the 40S small ribosomal subunit. It has a molecular mass of approximately 9.5 kDa. RPS21 is involved in the assembly and stability of ribosomal RNA. It is mostly expressed in the cytoplasm where it participates in the synthesis of proteins. Its expression levels vary between different cell types with significant presence in cells experiencing high rates of protein synthesis.

Biological function summary

The protein plays an important role as a structural constituent of ribosomes contributing to the translation process. It forms part of the ribosomal protein complex which is essential for ribosome biogenesis and function. The stability and functionality of the ribosomal protein complex rely on the presence of RPS21 affecting the overall efficiency of the protein translation machinery. This process is critical for normal cellular growth and division.

Pathways

The ribosome biogenesis and translation pathways are directly impacted by RPS21. It interacts closely with other ribosomal proteins and factors such as RPL5 and EIF4E. These interactions ensure the proper assembly of ribosomes and the initiation of translation. By playing an integral part in these pathways RPS21 influences cellular processes like cell growth maintenance and division.

Aberrations in RPS21 function associate with conditions such as Diamond-Blackfan anemia and certain cancers. Mutations or dysregulation affecting RPS21 can impact protein synthesis leading to these conditions. Other ribosomal proteins such as RPL11 and RPL35 also show involvement in these diseases suggesting a complex interaction network between ribosomal components in pathological states. Understanding RPS21's role in these diseases provides valuable insight into potential therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the small ribosomal subunit (PubMed : 23636399, PubMed : 25901680, PubMed : 25957688). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399, PubMed : 25901680, PubMed : 25957688).

See full target information RPS21

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Histology and histopathology 40:1295-1307 PubMed39698827

2024

TRIM22 mechanism promoting KAT2A ubiquitination degradation to regulate ferroptosis in hepatocellular carcinoma cell invasion and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wang,Xiaoshan Chen,Wei Wei

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPS21 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS21 antibody (AB254670)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS21 antibody (AB254670)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS21 antibody (AB254670)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS21 antibody (AB254670)

Immunocytochemistry/ Immunofluorescence - Anti-RPS21 antibody (AB254670)

Western blot - Anti-RPS21 antibody (AB254670)

Western blot - Anti-RPS21 antibody (AB254670)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise