Anti-14-3-3E / Tryptophan 5-Monooxygenase Antibody [CPTC-YWHAE-1]
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(2 Publications)
Mouse Monoclonal YWHAE antibody. Suitable for WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human YWHAE.
View Alternative Names
14-3-3 protein epsilon, 14-3-3E, YWHAE
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3E / Tryptophan 5-Monooxygenase Antibody [CPTC-YWHAE-1] (AB268130)
Formalin-fixed, paraffin-embedded human testis tissue stained for RPS23 using ab268130 at 2 μg/ml in immunohistochemical analysis.
- WB
Unknown
Western blot - Anti-14-3-3E / Tryptophan 5-Monooxygenase Antibody [CPTC-YWHAE-1] (AB268130)
All lanes:
Western blot - Anti-14-3-3E / Tryptophan 5-Monooxygenase Antibody [CPTC-YWHAE-1] (ab268130) at 2 µg/mL
Lane 1:
HEK293 (Human epithelial cell line from embryonic kidney) cell lysate
Lane 2:
PC-3 (Human prostate adenocarcinoma cell line) cell lysate
Lane 3:
A431 (Human epidermoid carcinoma cell line) cell lysate
Predicted band size: 16 kDa
false
- Protein Array
Unknown
Protein Array - Anti-14-3-3E / Tryptophan 5-Monooxygenase Antibody [CPTC-YWHAE-1] (AB268130)
Protein Array containing more than 19,000 full-length human proteins using ab268130.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Reactivity data
Properties and storage information
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Purification technique
Purification notes
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
RPS23 is critical in the assembly and structural stability of ribosomes. Within the ribosomal complex it contributes to the accurate decoding of messenger RNA (mRNA) during translation. RPS23 forms part of the small ribosomal subunit which recognizes and binds mRNA and transfer RNA (tRNA). Its position and structure facilitate correct alignment of the mRNA ensuring precise protein synthesis.
Pathways
RPS23 has essential roles in the protein synthesis pathway and is involved in the translation initiation complex. It interacts closely with other ribosomal proteins like RPS19 to enable the production of polypeptide chains from mRNA templates. Through this process RPS23 connects to cellular growth and differentiation pathways such as the mTOR signaling pathway which influences protein biosynthesis and cell survival.
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Target data
Publications (2)
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BMC nephrology 25:387 PubMed39478440
2024
Applications
Unspecified application
Species
Unspecified reactive species
Functional & integrative genomics 23:16 PubMed36562844
2022
Applications
Unspecified application
Species
Unspecified reactive species
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