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Mouse Monoclonal RPS23 antibody. Suitable for WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human 40S ribosomal protein S23.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS23 antibody [CPTC-YWHAE-1] (AB268130), expandable thumbnail
  • Protein Array - Anti-RPS23 antibody [CPTC-YWHAE-1] (AB268130), expandable thumbnail
  • Western blot - Anti-RPS23 antibody [CPTC-YWHAE-1] (AB268130), expandable thumbnail

Publications

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human 40S ribosomal protein S23. Database link P62266

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBProtein ArrayIHC-P
Human
Tested
Expected
Tested
Recombinant full length protein - Human
Not recommended
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1-2 µg/mL
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1-2 µg/mL
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:23636399, PubMed:25901680, PubMed:25957688, PubMed:28257692). The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules (PubMed:23636399, PubMed:25901680, PubMed:25957688). The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain (PubMed:23636399, PubMed:25901680, PubMed:25957688). The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel (PubMed:23636399, PubMed:25901680, PubMed:25957688). Plays an important role in translational accuracy (PubMed:28257692). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed:34516797).

Alternative names

Small ribosomal subunit protein uS12, 40S ribosomal protein S23, RPS23

Recommended products

Mouse Monoclonal RPS23 antibody. Suitable for WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human 40S ribosomal protein S23.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human 40S ribosomal protein S23. Database link P62266
Clone number
CPTC-YWHAE-1
Purification technique
Affinity purification Protein A/G
Light chain type
kappa
Concentration
Loading...
Purification notes

Purified from Bioreactor concentrate

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Ribosomal protein S23 (RPS23) is an important component of the 40S ribosomal subunit which plays a central role in protein synthesis. Also known simply as S23 this protein has a mass of approximately 15 kDa. RPS23 is ubiquitously expressed in different tissues reflecting its essential role in cellular function. It is highly conserved across species highlighting its importance in fundamental biological processes.

Biological function summary

RPS23 is critical in the assembly and structural stability of ribosomes. Within the ribosomal complex it contributes to the accurate decoding of messenger RNA (mRNA) during translation. RPS23 forms part of the small ribosomal subunit which recognizes and binds mRNA and transfer RNA (tRNA). Its position and structure facilitate correct alignment of the mRNA ensuring precise protein synthesis.

Pathways

RPS23 has essential roles in the protein synthesis pathway and is involved in the translation initiation complex. It interacts closely with other ribosomal proteins like RPS19 to enable the production of polypeptide chains from mRNA templates. Through this process RPS23 connects to cellular growth and differentiation pathways such as the mTOR signaling pathway which influences protein biosynthesis and cell survival.

Associated diseases and disorders

Abnormalities in RPS23 can lead to disorders such as ribosomopathies and cancer. Mutations in RPS23 affect ribosome function and efficiency linking it to diseases that involve rapid cell proliferation. In cancer alterations in ribosomal biogenesis often correlate with aggressive tumor growth where RPS23 may interact with proteins such as p53 that regulate cell cycle and apoptosis. This highlights the significance of maintaining normal RPS23 functions for cellular homeostasis and disease prevention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS23 antibody [CPTC-YWHAE-1] (ab268130), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS23 antibody [CPTC-YWHAE-1] (ab268130)

    Formalin-fixed, paraffin-embedded human testis tissue stained for RPS23 using ab268130 at 2 μg/ml in immunohistochemical analysis.

  • Protein Array - Anti-RPS23 antibody [CPTC-YWHAE-1] (ab268130), expandable thumbnail

    Protein Array - Anti-RPS23 antibody [CPTC-YWHAE-1] (ab268130)

    Protein Array containing more than 19,000 full-length human proteins using ab268130.

    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

  • Western blot - Anti-RPS23 antibody [CPTC-YWHAE-1] (ab268130), expandable thumbnail

    Western blot - Anti-RPS23 antibody [CPTC-YWHAE-1] (ab268130)

    All lanes: Western blot - Anti-RPS23 antibody [CPTC-YWHAE-1] (ab268130) at 2 µg/mL

    Lane 1: HEK293 (Human epithelial cell line from embryonic kidney) cell lysate

    Lane 2: PC-3 (Human prostate adenocarcinoma cell line) cell lysate

    Lane 3: A431 (Human epidermoid carcinoma cell line) cell lysate

    Predicted band size: 16 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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