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AB196652

Anti-RPS24 antibody [EPR16017(B)]

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(16 Publications)

Rabbit Recombinant Monoclonal RPS24 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 16 publications.

View Alternative Names

Small ribosomal subunit protein eS24, 40S ribosomal protein S24, RPS24

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS24 antibody [EPR16017(B)] (AB196652)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS24 antibody [EPR16017(B)] (AB196652)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling RPS24 with ab196652 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on Human tonsil tissue is observed. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-RPS24 antibody [EPR16017(B)] (AB196652)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RPS24 antibody [EPR16017(B)] (AB196652)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized SH-SY5Y (Human neuroblastoma from bone marrow cells) cells labeling RPS24 with ab196652 at 1/250 dilution followed by AlexaFluor®488 Goat anti-Rabbit (ab150077) secondary antibody at 1/400 dilution (green). Cytoplasm staining on SH-SY5Y cell line is observed. The nuclear counter stain is DAPI (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS24 antibody [EPR16017(B)] (AB196652)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS24 antibody [EPR16017(B)] (AB196652)

Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma of cervix tissue labeling RPS24 with ab196652 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on Human squamous cell carcinoma of cervix tissue is observed. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS24 antibody [EPR16017(B)] (AB196652)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS24 antibody [EPR16017(B)] (AB196652)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling RPS24 with ab196652 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on mouse liver tissue is observed. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western blot - Anti-RPS24 antibody [EPR16017(B)] (AB196652)
  • WB

Supplier Data

Western blot - Anti-RPS24 antibody [EPR16017(B)] (AB196652)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RPS24 antibody [EPR16017(B)] (ab196652) at 1/5000 dilution

Lane 1:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 10 µg

Lane 2:

293T (Human epithelial cells from embryonic kidney) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 15 kDa

Observed band size: 15 kDa

false

Western blot - Anti-RPS24 antibody [EPR16017(B)] (AB196652)
  • WB

Supplier Data

Western blot - Anti-RPS24 antibody [EPR16017(B)] (AB196652)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RPS24 antibody [EPR16017(B)] (ab196652) at 1/1000 dilution

Lane 1:

Mouse colon tissue lysate at 10 µg

Lane 2:

Rat colon tissue lysate at 10 µg

Lane 3:

Mouse liver lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 15 kDa

Observed band size: 15 kDa

false

  • Carrier free

    Anti-RPS24 antibody [EPR16017(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16017(B)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPS24 also known as Ribosomal Protein S24 is a component of the 40S ribosomal subunit. It has a molecular mass of approximately 15 kDa. RPS24 is an integral part of the small ribosomal subunit and it is expressed in various tissues highlighting its comprehensive involvement in protein synthesis. As a ribosomal protein RPS24 assists in the translation of mRNA into polypeptide chains by contributing to the correct assembly and function of the ribosome.
Biological function summary

Understanding the function of ribosomal proteins like RPS24 offers insights into protein synthesis. RPS24 achieves its function as part of the 40S ribosomal subunit a critical component of the protein translation machinery in cells. By being part of this complex RPS24 helps in decoding mRNA thereby ensuring the proper translation of the genetic code into proteins. This process is vital for maintaining cellular function and growth supporting the various roles proteins perform within the organism.

Pathways

RPS24 plays a role in the ribosome biogenesis and translation pathways. RPS24 is closely linked with proteins like RPL5 and RPL11 which act within these pathways to regulate ribosome assembly and function. These pathways involve a series of highly coordinated steps controlling ribosome production and activity essential for cell growth and division. By participating in these pathways RPS24 indirectly supports numerous other cellular processes dependent on efficient protein synthesis.

Defects in RPS24 have been associated with Diamond-Blackfan Anemia a rare genetic disorder that affects the bone marrow. RPS24 mutations can impair ribosome function leading to ineffective production of red blood cells. This association with disease highlights the importance of RPS24 in normal hematopoiesis. Additionally RPS24 interacts with proteins such as RPL11 where alterations may contribute to the pathology of this anemia further emphasizing the relevance of RPS24 in both normal and disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the small ribosomal subunit (PubMed : 23636399). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399). Required for processing of pre-rRNA and maturation of 40S ribosomal subunits (PubMed : 18230666). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).
See full target information RPS24
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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:9963 PubMed39551769

2024

Soluble αβ-tubulins reversibly sequester TTC5 to regulate tubulin mRNA decay.

Applications

Unspecified application

Species

Unspecified reactive species

Alina Batiuk,Markus Höpfler,Ana C Almeida,Deryn Teoh En-Jie,Oscar Vadas,Evangelia Vartholomaiou,Ramanujan S Hegde,Zhewang Lin,Ivana Gasic

The EMBO journal 43:6555-6572 PubMed39533057

2024

LARP1 binds ribosomes and TOP mRNAs in repressed complexes.

Applications

Unspecified application

Species

Unspecified reactive species

James A Saba,Zixuan Huang,Kate L Schole,Xianwen Ye,Shrey D Bhatt,Yi Li,Winston Timp,Jingdong Cheng,Rachel Green

Nature communications 15:6633 PubMed39117603

2024

Human tumor suppressor protein Pdcd4 binds at the mRNA entry channel in the 40S small ribosomal subunit.

Applications

Unspecified application

Species

Unspecified reactive species

Jailson Brito Querido,Masaaki Sokabe,Irene Díaz-López,Yuliya Gordiyenko,Philipp Zuber,Yifei Du,Lucas Albacete-Albacete,V Ramakrishnan,Christopher S Fraser

Cell 187:3652-3670.e40 PubMed38843833

2024

The ribotoxic stress response drives UV-mediated cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Niladri K Sinha,Connor McKenney,Zhong Y Yeow,Jeffrey J Li,Ki Hong Nam,Tomer M Yaron-Barir,Jared L Johnson,Emily M Huntsman,Lewis C Cantley,Alban Ordureau,Sergi Regot,Rachel Green

Nature chemical biology 20:877-884 PubMed38172604

2024

The eRF1 degrader SRI-41315 acts as a molecular glue at the ribosomal decoding center.

Applications

Unspecified application

Species

Unspecified reactive species

João P L Coelho,Matthew C J Yip,Keely Oltion,Jack Taunton,Sichen Shao

Molecular cell 83:2290-2302.e13 PubMed37295431

2023

Mechanism of ribosome-associated mRNA degradation during tubulin autoregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Markus Höpfler,Eva Absmeier,Sew-Yeu Peak-Chew,Evangelia Vartholomaiou,Lori A Passmore,Ivana Gasic,Ramanujan S Hegde

International journal of molecular sciences 24: PubMed36614058

2022

Ribosomal Stress Couples with the Hypoxia Response in Dec1-Dependent Orthodontic Tooth Movement.

Applications

Unspecified application

Species

Unspecified reactive species

Shigeru Nakamura,Keiji Tanimoto,Ujjal K Bhawal

Cells 10: PubMed34203326

2021

Cockayne Syndrome-Associated CSA and CSB Mutations Impair Ribosome Biogenesis, Ribosomal Protein Stability, and Global Protein Folding.

Applications

Unspecified application

Species

Unspecified reactive species

Mingyue Qiang,Fatima Khalid,Tamara Phan,Christina Ludwig,Karin Scharffetter-Kochanek,Sebastian Iben

Molecular cell 81:2808-2822.e10 PubMed34111399

2021

Translation stress and collided ribosomes are co-activators of cGAS.

Applications

Unspecified application

Species

Unspecified reactive species

Li Wan,Szymon Juszkiewicz,Daniel Blears,Prashanth Kumar Bajpe,Zhong Han,Peter Faull,Richard Mitter,Aengus Stewart,Ambrosius P Snijders,Ramanujan S Hegde,Jesper Q Svejstrup

Molecular cell 81:2693-2704.e12 PubMed33964204

2021

WNK1 is an assembly factor for the human ER membrane protein complex.

Applications

Unspecified application

Species

Unspecified reactive species

Tino Pleiner,Masami Hazu,Giovani Pinton Tomaleri,Kurt Januszyk,Robert S Oania,Michael J Sweredoski,Annie Moradian,Alina Guna,Rebecca M Voorhees
View all publications
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Product promise

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