Rabbit Recombinant Monoclonal RPS24 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 10 publications.
Images
![Western blot - Anti-RPS24 antibody [EPR16017(B)] (AB196652), expandable thumbnail](https://content.abcam.com/products/images/rps24-antibody-epr16017b-ab196652--western-blot-img158129.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RPS24 antibody [EPR16017(B)] (AB196652)")
![Western blot - Anti-RPS24 antibody [EPR16017(B)] (AB196652), expandable thumbnail](https://content.abcam.com/products/images/rps24-antibody-epr16017b-ab196652--western-blot-img61892.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RPS24 antibody [EPR16017(B)] (AB196652)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS24 antibody [EPR16017(B)] (AB196652), expandable thumbnail](https://content.abcam.com/products/images/rps24-antibody-epr16017b-ab196652--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img33155.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS24 antibody [EPR16017(B)] (AB196652)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS24 antibody [EPR16017(B)] (AB196652), expandable thumbnail](https://content.abcam.com/products/images/rps24-antibody-epr16017b-ab196652--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114604.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS24 antibody [EPR16017(B)] (AB196652)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS24 antibody [EPR16017(B)] (AB196652), expandable thumbnail](https://content.abcam.com/products/images/rps24-antibody-epr16017b-ab196652--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img127750.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS24 antibody [EPR16017(B)] (AB196652)")
Publications
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- International journal of molecular sciences 24:2022Ribosomal Stress Couples with the Hypoxia Response in Dec1-Dependent Orthodontic Tooth Movement.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShigeru Nakamura,Keiji Tanimoto,Ujjal K BhawalPubMed 36614058
- Cells 10:2021Cockayne Syndrome-Associated CSA and CSB Mutations Impair Ribosome Biogenesis, Ribosomal Protein Stability, and Global Protein Folding.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingyue Qiang,Fatima Khalid,Tamara Phan,Christina Ludwig,Karin Scharffetter-Kochanek,Sebastian IbenPubMed 34203326
- Molecular cell 81:2808-2822.e102021Translation stress and collided ribosomes are co-activators of cGAS.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Wan,Szymon Juszkiewicz,Daniel Blears,Prashanth Kumar Bajpe,Zhong Han,Peter Faull,Richard Mitter,Aengus Stewart,Ambrosius P Snijders,Ramanujan S Hegde,Jesper Q SvejstrupPubMed 34111399
- Molecular cell 81:2693-2704.e122021WNK1 is an assembly factor for the human ER membrane protein complex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTino Pleiner,Masami Hazu,Giovani Pinton Tomaleri,Kurt Januszyk,Robert S Oania,Michael J Sweredoski,Annie Moradian,Alina Guna,Rebecca M VoorheesPubMed 33964204
- eLife 9:2020Ribosome collisions trigger cis-acting feedback inhibition of translation initiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSzymon Juszkiewicz et. al.PubMed 32657267
- Molecular cell 79:603-614.e82020The ASC-1 Complex Disassembles Collided Ribosomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSzymon Juszkiewicz et. al.PubMed 32579943
- Cell 175:1507-1519.e162018EMC Is Required to Initiate Accurate Membrane Protein Topogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPatrick J Chitwood et. al.PubMed 30415835
- eLife 7:2018Structures of translationally inactive mammalian ribosomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlan Brown et. al.PubMed 30355441
- Molecular cell 72:469-481.e72018ZNF598 Is a Quality Control Sensor of Collided Ribosomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSzymon Juszkiewicz et. al.PubMed 30293783
- Molecular cell 65:743-750.e42017Initiation of Quality Control during Poly(A) Translation Requires Site-Specific Ribosome Ubiquitination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSzymon Juszkiewicz et. al.PubMed 28065601
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Tested |
| Rat | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/1000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/50 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Component of the small ribosomal subunit (PubMed:23636399). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:23636399). Required for processing of pre-rRNA and maturation of 40S ribosomal subunits (PubMed:18230666). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed:34516797).
Alternative names
Small ribosomal subunit protein eS24, 40S ribosomal protein S24, RPS24
Recommended products
Rabbit Recombinant Monoclonal RPS24 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 10 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR16017(B)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RPS24 also known as Ribosomal Protein S24 is a component of the 40S ribosomal subunit. It has a molecular mass of approximately 15 kDa. RPS24 is an integral part of the small ribosomal subunit and it is expressed in various tissues highlighting its comprehensive involvement in protein synthesis. As a ribosomal protein RPS24 assists in the translation of mRNA into polypeptide chains by contributing to the correct assembly and function of the ribosome.
- Biological function summary
Understanding the function of ribosomal proteins like RPS24 offers insights into protein synthesis. RPS24 achieves its function as part of the 40S ribosomal subunit a critical component of the protein translation machinery in cells. By being part of this complex RPS24 helps in decoding mRNA thereby ensuring the proper translation of the genetic code into proteins. This process is vital for maintaining cellular function and growth supporting the various roles proteins perform within the organism.
- Pathways
RPS24 plays a role in the ribosome biogenesis and translation pathways. RPS24 is closely linked with proteins like RPL5 and RPL11 which act within these pathways to regulate ribosome assembly and function. These pathways involve a series of highly coordinated steps controlling ribosome production and activity essential for cell growth and division. By participating in these pathways RPS24 indirectly supports numerous other cellular processes dependent on efficient protein synthesis.
- Associated diseases and disorders
Defects in RPS24 have been associated with Diamond-Blackfan Anemia a rare genetic disorder that affects the bone marrow. RPS24 mutations can impair ribosome function leading to ineffective production of red blood cells. This association with disease highlights the importance of RPS24 in normal hematopoiesis. Additionally RPS24 interacts with proteins such as RPL11 where alterations may contribute to the pathology of this anemia further emphasizing the relevance of RPS24 in both normal and disease states.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-RPS24 antibody [EPR16017(B)] (ab196652), expandable thumbnail](https://content.abcam.com/products/images/rps24-antibody-epr16017b-ab196652--western-blot-img158129.jpg "Western blot - Anti-RPS24 antibody [EPR16017(B)] (ab196652)")
Western blot - Anti-RPS24 antibody [EPR16017(B)] (ab196652)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-RPS24 antibody [EPR16017(B)] (ab196652) at 1/5000 dilution
Lane 1: Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 10 µg
Lane 2: 293T (Human epithelial cells from embryonic kidney) whole cell lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa
![Western blot - Anti-RPS24 antibody [EPR16017(B)] (ab196652), expandable thumbnail](https://content.abcam.com/products/images/rps24-antibody-epr16017b-ab196652--western-blot-img61892.jpg "Western blot - Anti-RPS24 antibody [EPR16017(B)] (ab196652)")
Western blot - Anti-RPS24 antibody [EPR16017(B)] (ab196652)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-RPS24 antibody [EPR16017(B)] (ab196652) at 1/1000 dilution
Lane 1: Mouse colon tissue lysate at 10 µg
Lane 2: Rat colon tissue lysate at 10 µg
Lane 3: Mouse liver lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS24 antibody [EPR16017(B)] (ab196652), expandable thumbnail](https://content.abcam.com/products/images/rps24-antibody-epr16017b-ab196652--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img33155.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS24 antibody [EPR16017(B)] (ab196652)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS24 antibody [EPR16017(B)] (ab196652)
Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma of cervix tissue labeling RPS24 with ab196652 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on Human squamous cell carcinoma of cervix tissue is observed. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS24 antibody [EPR16017(B)] (ab196652), expandable thumbnail](https://content.abcam.com/products/images/rps24-antibody-epr16017b-ab196652--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114604.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS24 antibody [EPR16017(B)] (ab196652)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS24 antibody [EPR16017(B)] (ab196652)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling RPS24 with ab196652 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on Human tonsil tissue is observed. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS24 antibody [EPR16017(B)] (ab196652), expandable thumbnail](https://content.abcam.com/products/images/rps24-antibody-epr16017b-ab196652--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img127750.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS24 antibody [EPR16017(B)] (ab196652)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS24 antibody [EPR16017(B)] (ab196652)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling RPS24 with ab196652 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on mouse liver tissue is observed. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-RPS24 antibody [EPR16017(B)] (ab196652), expandable thumbnail](https://content.abcam.com/products/images/rps24-antibody-epr16017b-ab196652--immunocytochemistry-immunofluorescence-img53977.jpg "Immunocytochemistry/ Immunofluorescence - Anti-RPS24 antibody [EPR16017(B)] (ab196652)")
Immunocytochemistry/ Immunofluorescence - Anti-RPS24 antibody [EPR16017(B)] (ab196652)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (Human neuroblastoma from bone marrow cells) cells labeling RPS24 with ab196652 at 1/250 dilution, followed by AlexaFluor®488 Goat anti-Rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/400 dilution (green). Cytoplasm staining on SH-SY5Y cell line is observed. The nuclear counter stain is DAPI (blue).
![Immunocytochemistry/ Immunofluorescence - Anti-RPS24 antibody [EPR16017(B)] (ab196652), expandable thumbnail](https://content.abcam.com/products/images/rps24-antibody-epr16017b-ab196652--immunocytochemistry-immunofluorescence-img53977.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-RPS24 antibody [EPR16017(B)] (ab196652)")
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