AB229571

Anti-RPS26 antibody - N-terminal

Name: Anti-RPS26 antibody - N-terminal (ab229571) | Abcam
Brand: Abcam Limited
SKU: AB229571

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(1 Publication)

Rabbit Polyclonal RPS26 antibody. N-terminal. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human 40S ribosomal protein S26.

View Alternative Names

Small ribosomal subunit protein eS26, 40S ribosomal protein S26, RPS26

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-RPS26 antibody - N-terminal (AB229571)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RPS26 antibody - N-terminal (AB229571)

4% paraformaldehyde-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for RPS26 (green) using ab229571 at 1/500 dilution in ICC/IF.

Blue : Hoechst 33342 staining.

Supplier Data

Western blot - Anti-RPS26 antibody - N-terminal (AB229571)

15% SDS-PAGE gel.

All lanes:

Western blot - Anti-RPS26 antibody - N-terminal (ab229571) at 1/500 dilution

All lanes:

MCF7 (human breast adenocarcinoma cell line) whole cell extract at 30 µg

Predicted band size: 13 kDa

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Supplier Data

Western blot - Anti-RPS26 antibody - N-terminal (AB229571)

15% SDS-PAGE gel.

All lanes:

Western blot - Anti-RPS26 antibody - N-terminal (ab229571) at 1/500 dilution

All lanes:

Rat2 (rat fibroblast cell line) whole cell extract at 30 µg

Predicted band size: 13 kDa

false

Supplier Data

Western blot - Anti-RPS26 antibody - N-terminal (AB229571)

15% SDS-PAGE gel.

All lanes:

Western blot - Anti-RPS26 antibody - N-terminal (ab229571) at 1/500 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma cell line) whole cell extract at 30 µg

Lane 2:

C8D30 whole cell extract at 30 µg

Lane 3:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract at 30 µg

Lane 4:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract at 30 µg

Lane 5:

C2C12 (mouse myoblast cell line) whole cell extract at 30 µg

Predicted band size: 13 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human 40S ribosomal protein S26. The exact immunogen used to generate this antibody is proprietary information.

P62854

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPS26 also known as ribosomal protein S26 is a part of the ribosomal machinery involved in protein synthesis. This small protein has a molecular weight of approximately 14 kDa. RPS26 is widely expressed in various tissues indicating its functional importance in cellular processes. As a component of the 40S ribosomal subunit it plays a role in the translation process by contributing to the assembly and structure of the ribosome.

Biological function summary

RPS26 functions as a fundamental component of the ribosome where it interacts with ribosomal RNA and other ribosomal proteins to facilitate protein synthesis. This protein is part of the ribonucleoprotein complex essential for translating mRNA into polypeptides. The involvement of RPS26 in the intricate ribosomal machinery ensures the accurate and efficient production of cellular proteins that drive numerous cellular activities.

Pathways

RPS26 contributes significantly to protein synthesis pathways notably engaging in the translation initiation pathway. In this pathway RPS26 closely interacts with other ribosomal proteins such as RPS20 and RPS21. Its involvement in ribosome biogenesis pathways also plays a critical role in cellular growth and proliferation further connecting its function to essential cellular processes.

Mutations or disruptions in RPS26 can be associated with Diamond-Blackfan Anemia a disorder that affects red blood cell production. This connection highlights the sensitivity of erythropoiesis to perturbations in ribosomal function. Additionally changes in RPS26 expression levels also hint at its potential involvement in various cancers where it might interact with proteins like MDM2 influencing tumor suppression pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the small ribosomal subunit (PubMed : 23636399, PubMed : 25901680, PubMed : 25957688). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399, PubMed : 25901680, PubMed : 25957688).

See full target information 40S ribosomal protein S26

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 136: PubMed37701987

2023

Loss of ATG4B and ATG4A results in two-stage cell cycle defects in pancreatic ductal adenocarcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Paalini Sathiyaseelan,Suganthi Chittaranjan,Steve E Kalloger,Jennifer Chan,Nancy E Go,Mario A Jardon,Cally J Ho,Theodore Hui,Jing Xu,Christine Chow,Dongxia Gao,Fraser D Johnson,William W Lockwood,Gregg B Morin,Daniel J Renouf,David F Schaeffer,Sharon M Gorski

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPS26 antibody - N-terminal

Immunocytochemistry/ Immunofluorescence - Anti-RPS26 antibody - N-terminal (AB229571)

Western blot - Anti-RPS26 antibody - N-terminal (AB229571)

Western blot - Anti-RPS26 antibody - N-terminal (AB229571)

Western blot - Anti-RPS26 antibody - N-terminal (AB229571)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise