AB197382

Anti-RPS27/MPS1 antibody

Name: Anti-RPS27/MPS1 antibody (ab197382) | Abcam
Brand: Abcam Limited
SKU: AB197382

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(2 Publications)

Rabbit Polyclonal RPS27/MPS1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications.

View Alternative Names

MPS1, RPS27, Small ribosomal subunit protein eS27, 40S ribosomal protein S27, Metallopan-stimulin 1, MPS-1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS27/MPS1 antibody (AB197382)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS27/MPS1 antibody (AB197382)

Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue labeling RPS27/MPS1 with ab197382 at a 1/25 dilution.

Supplier Data

Western blot - Anti-RPS27/MPS1 antibody (AB197382)

12%SDS-PAGE

All lanes:

Western blot - Anti-RPS27/MPS1 antibody (ab197382) at 1/300 dilution

Lane 1:

Jurkat cell lysate at 40 µg

Lane 2:

K562 cell lysate at 40 µg

Lane 3:

Raji cell lysate at 40 µg

Predicted band size: 9 kDa

false

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/25 - 1/100", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPS27 also known as MPS1 is a ribosomal protein that is an essential component of the 40S subunit of the ribosome. With a molecular mass of approximately 9 kDa RPS27/MPS1 plays a vital role in protein synthesis. This protein is expressed in multiple tissues including highly proliferative cells such as those found in the bone marrow and lymphoid tissues. Its expression level is generally correlated with cell growth and proliferation rates.

Biological function summary

RPS27/MPS1 is important for ribosome assembly and function. It participates in the stabilization of ribosomal structures. This protein is often found as part of a larger ribosome complex working together with other ribosomal proteins and rRNA to ensure accurate translation of mRNA into proteins. The assembly and function of ribosomes are fundamental for cell growth and division marking RPS27/MPS1 as significant in these processes.

Pathways

RPS27/MPS1 is involved in critical translation initiation and ribosomal biogenesis pathways. It interacts with proteins involved in the mTOR signaling pathway which regulates cell growth and metabolism in response to nutrients and energy status. Additionally it relates to the nucleolus stress response a pathway linked with protein synthesis and cellular stress responses. MPS1 works either directly or indirectly with proteins such as mTOR and p53 impacting cellular homeostasis and growth control mechanisms.

RPS27/MPS1 has been connected to cancer and ribosomopathies. Abnormal expressions or mutations in RPS27/MPS1 can contribute to tumorigenesis due to its role in unchecked cell proliferation. Dysregulation of this protein is associated with breast cancer where it may interact with oncogenes and tumor suppressor genes like p53 and Bcl-2. Additionally changes in RPS27/MPS1 expression or mutation have implications in ribosomopathies a group of diseases characterized by defective ribosome function. These connections highlight the importance of understanding RPS27/MPS1 in disease pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the small ribosomal subunit (PubMed : 23636399, PubMed : 8706699). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399). Required for proper rRNA processing and maturation of 18S rRNAs (PubMed : 25424902). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).

See full target information RPS27

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Reproductive biology and endocrinology : RB&E 19:111 PubMed34266426

2021

Overexpressed MPS-1 contributes to endometrioma development through the NF-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Liu,Junyan Ma,Liqi Zhang,Jun Lin,Xiaohua Liu

Life science alliance 2: PubMed31792062

2019

ADAP1 promotes invasive squamous cell carcinoma progression and predicts patient survival.

Applications

Unspecified application

Species

Unspecified reactive species

Avery Van Duzer,Sachiko Taniguchi,Ajit Elhance,Takahiro Tsujikawa,Naoki Oshimori

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPS27/MPS1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS27/MPS1 antibody (AB197382)

Western blot - Anti-RPS27/MPS1 antibody (AB197382)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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