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AB221904

Anti-RPS27/MPS1 + RPS27L antibody

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(1 Publication)

Rabbit Polyclonal RPS27L antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RPS27 aa 1-50.

View Alternative Names

Ribosomal protein eS27-like, 40S ribosomal protein S27-like, Small ribosomal subunit protein eS27-like, RPS27L, MPS1, RPS27, Small ribosomal subunit protein eS27, 40S ribosomal protein S27, Metallopan-stimulin 1, MPS-1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-RPS27/MPS1 + RPS27L antibody (AB221904)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RPS27/MPS1 + RPS27L antibody (AB221904)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for RPS27L (green) using ab221904 at 4 μg/ml in ICC/IF.

Western blot - Anti-RPS27/MPS1 + RPS27L antibody (AB221904)
  • WB

Supplier Data

Western blot - Anti-RPS27/MPS1 + RPS27L antibody (AB221904)

All lanes:

Western blot - Anti-RPS27/MPS1 + RPS27L antibody (ab221904) at 0.4 µg/mL

All lanes:

THP-1 cell lysate

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human RPS27 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P42677

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPS27 also known as MPS1 or RPS27L is a ribosomal protein with a molecular weight of approximately 9.6 kDa. This protein is expressed in a wide range of tissues and contributes to the structural and functional integrity of the 40S ribosomal subunit. RPS27 is an integral component of ribosome biogenesis playing a role in the assembly of ribosomal proteins and rRNA necessary for protein synthesis.
Biological function summary

RPS27 performs functions beyond its structural component of the ribosome. It engages in the regulation of cell proliferation and apoptosis acting as part of the molecular machinery that ensures cell cycle progression and programmed cell death. RPS27 interacts with other ribosomal proteins and non-ribosomal factors suggesting its involvement in a larger protein complex that maintains cellular homeostasis. This protein can influence p53-mediated responses highlighting its roles in important cellular processes.

Pathways

RPS27 integrates into the p53 signaling pathway and the mTOR signaling pathway. These pathways regulate vital functions such as cell growth cell cycle and response to DNA damage. RPS27 aligns its activities with proteins like MDM2 and S6K1 which are critical in managing cell survival and metabolic regulation. Its interaction in these pathways positions RPS27 as a regulatory node in controlling cell fate decisions and response to cellular stressors.

RPS27 shows relevance in cancer and anemias. Altered expression or mutations in this protein have associations with tumorigenesis connecting it with cancer-related proteins such as p53 and MDM2. Its involvement in the regulation of erythropoiesis links it to anemias especially those arising from ribosome biogenesis defects. These associations establish RPS27 as a significant target for understanding and potentially addressing these medical conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information 40S ribosomal protein S27-like

Additional targets

RPS27
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of oncology 2022:2378253 PubMed36090903

2022

CKAP2L Promotes Esophageal Squamous Cell Carcinoma Progression and Drug-Resistance by Modulating Cell Cycle.

Applications

Unspecified application

Species

Unspecified reactive species

Wenhu Chen,Yu Wang,Lifang Wang,Hongguang Zhao,Xiaoyan Li
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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