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AB241282

Anti-RPS28 antibody

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(1 Publication)

Rabbit Polyclonal RPS28 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RPS28 aa 1 to C-terminus.

View Alternative Names

Small ribosomal subunit protein eS28, 40S ribosomal protein S28, RPS28

2 Images
Immunoprecipitation - Anti-RPS28 antibody (AB241282)
  • IP

Supplier Data

Immunoprecipitation - Anti-RPS28 antibody (AB241282)

RPS28 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241282 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab241282 at 0.4 μg/ml.

Lane 1 : ab241282 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-RPS28 antibody (ab241282)

Predicted band size: 8 kDa

false

Western blot - Anti-RPS28 antibody (AB241282)
  • WB

Supplier Data

Western blot - Anti-RPS28 antibody (AB241282)

All lanes:

Western blot - Anti-RPS28 antibody (ab241282) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) cell lysate at 50 µg

Predicted band size: 8 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human RPS28 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P62857

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/25000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/25000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab241282 was affinity purified using an epitope specific to RPS28 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPS28 also known as ribosomal protein S28 is a component of the 40S subunit of the ribosome. The protein has a molecular mass of about 7.6 kDa and is part of the S28E family of ribosomal proteins. RPS28 is highly expressed in various tissues reflecting its importance in cells that are actively synthesizing proteins. This protein plays a mechanical role in ribosome function by participating in the assembly of ribosomal structures and facilitating translation.
Biological function summary

Ribosomal proteins like RPS28 are essential for protein biosynthesis. RPS28 is a part of the ribosomal small subunit complex which helps decode messenger RNA (mRNA) into polypeptides. Proper functioning of the ribosome ensures the accurate translation of genetic material into proteins which is necessary for cell growth and maintenance. The protein cooperates with other ribosomal proteins to maintain the integrity and functionality of the ribosomal machinery.

Pathways

RPS28 is involved in the translation pathway which is critical for gene expression. It contributes to the initiation phase of translation working alongside the eukaryotic initiation factor 3 (eIF3) complex. The protein also plays a role in the maturation of ribosomal RNA (rRNA) within the ribosome biogenesis pathway. Through these pathways RPS28 interacts with other ribosomal proteins such as RPS15 and RPS23 which are essential for the assembly and function of the ribosome.

Mutations in RPS28 have been linked to Diamond-Blackfan anemia a rare genetic condition characterized by bone marrow failure and anemia. This disorder demonstrates the protein's importance in hematopoiesis and red blood cell production. Additionally researchers are examining connections between RPS28 and its interaction with the p53 pathway given p53's role in tumor suppression and the DNA damage response suggesting potential implications in certain cancers. Understanding these interactions may contribute to better insights into the molecular basis of these diseases and potential therapeutic approaches.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the small ribosomal subunit (PubMed : 23636399, PubMed : 25901680, PubMed : 25957688). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399, PubMed : 25901680, PubMed : 25957688). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).
See full target information RPS28
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Translational oncology 58:102423 PubMed40414119

2025

GLI2-HRD1 axis facilitates 5-FU resistance in gastric cancer cells by regulating ubiquitination degradation of UCK2.

Applications

Unspecified application

Species

Unspecified reactive species

Chaorong Xue,Xuanzi Zhang,Wanling Yu,Hanbin Lin,Junrong Zhang,Jiawen Liu,Zongqi Weng,Manduo Ouyang,Xinjian Lin
View all publications
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