AB241405

Anti-RPS28 antibody

Name: Anti-RPS28 antibody (ab241405) | Abcam
Brand: Abcam Limited
SKU: AB241405
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal RPS28 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RPS28 aa 1 to C-terminus.

View Alternative Names

Small ribosomal subunit protein eS28, 40S ribosomal protein S28, RPS28

2 Images

Supplier Data

Immunoprecipitation - Anti-RPS28 antibody (AB241405)

RPS28 was immunopreciptated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab241405 at 6 μg per reaction. Western blot was performed on the immunoprecipitate using ab241405 at 0.4 μg/mL.

Lane 1 : ab241405 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with an exposure time of 10 seconds.
Prepared using NETN lysis buffer.

All lanes:

Immunoprecipitation - Anti-RPS28 antibody (ab241405)

Predicted band size: 8 kDa

false

Supplier Data

Western blot - Anti-RPS28 antibody (AB241405)

Prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-RPS28 antibody (ab241405) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 8 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human RPS28 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P62857

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/25000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/25000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Drosophila melanogaster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antibody was affinity purified using an epitope specific to RPS28 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPS28 also known as ribosomal protein S28 is a component of the 40S subunit of the ribosome. The protein has a molecular mass of about 7.6 kDa and is part of the S28E family of ribosomal proteins. RPS28 is highly expressed in various tissues reflecting its importance in cells that are actively synthesizing proteins. This protein plays a mechanical role in ribosome function by participating in the assembly of ribosomal structures and facilitating translation.

Biological function summary

Ribosomal proteins like RPS28 are essential for protein biosynthesis. RPS28 is a part of the ribosomal small subunit complex which helps decode messenger RNA (mRNA) into polypeptides. Proper functioning of the ribosome ensures the accurate translation of genetic material into proteins which is necessary for cell growth and maintenance. The protein cooperates with other ribosomal proteins to maintain the integrity and functionality of the ribosomal machinery.

Pathways

RPS28 is involved in the translation pathway which is critical for gene expression. It contributes to the initiation phase of translation working alongside the eukaryotic initiation factor 3 (eIF3) complex. The protein also plays a role in the maturation of ribosomal RNA (rRNA) within the ribosome biogenesis pathway. Through these pathways RPS28 interacts with other ribosomal proteins such as RPS15 and RPS23 which are essential for the assembly and function of the ribosome.

Mutations in RPS28 have been linked to Diamond-Blackfan anemia a rare genetic condition characterized by bone marrow failure and anemia. This disorder demonstrates the protein's importance in hematopoiesis and red blood cell production. Additionally researchers are examining connections between RPS28 and its interaction with the p53 pathway given p53's role in tumor suppression and the DNA damage response suggesting potential implications in certain cancers. Understanding these interactions may contribute to better insights into the molecular basis of these diseases and potential therapeutic approaches.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the small ribosomal subunit (PubMed : 23636399, PubMed : 25901680, PubMed : 25957688). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399, PubMed : 25901680, PubMed : 25957688). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).

See full target information RPS28

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 41:17 PubMed34998399

2022

The RNA-binding protein GRSF1 promotes hepatocarcinogenesis via competitively binding to YY1 mRNA with miR-30e-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Han,Chen Huang,Xiaofei Wang,Dongdong Tong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-RPS28 antibody

Immunoprecipitation - Anti-RPS28 antibody (AB241405)

Western blot - Anti-RPS28 antibody (AB241405)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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