AB140688

Anti-RPS3 antibody

Name: Anti-RPS3 antibody (ab140688) | Abcam
Brand: Abcam Limited
SKU: AB140688
Price: 535 USD
Availability: InStock

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(10 Publications)

Rabbit Polyclonal RPS3 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human RPS3 aa 150 to C-terminus.

View Alternative Names

OK/SW-cl.26, RPS3, Small ribosomal subunit protein uS3, 40S ribosomal protein S3

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS3 antibody (AB140688)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS3 antibody (AB140688)

Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue sections using ab117809 at 1/1000 dilution.

Unknown

Immunoprecipitation - Anti-RPS3 antibody (AB140688)

Detection of RPS3 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab140688 at 6 µg/mg lysate for IP (Lane 1) and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-RPS3 antibody (ab140688)

Predicted band size: 27 kDa

false

Unknown

Western blot - Anti-RPS3 antibody (AB140688)

All lanes:

Western blot - Anti-RPS3 antibody (ab140688) at 0.1 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 27 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP, IHC-P

applications

Immunogen

Synthetic Peptide within Human RPS3 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P23396

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "African bush elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Corn": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Salamander": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Spotted catshark": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPS3 also known as Ribosomal Protein S3 is an important component of the 40S ribosomal subunit. It has a molecular mass of approximately 26 kDa. Functionally RPS3 plays a role in the translation process as part of the ribosome machinery assisting in the assembly of proteins by decoding messenger RNA. Expression of RPS3 is ubiquitous across various tissues suggesting a fundamental role for the protein in cellular functions.

Biological function summary

Protein synthesis relies heavily on components like RPS3 within the ribosomal complex where it interacts with other ribosomal proteins and rRNA to ensure accurate translation. RPS3 also exhibits DNA repair activities independently of its ribosomal function. It binds to DNA damaged sites adding a layer of protection to help maintain genomic stability by facilitating repair processes.

Pathways

RPS3 participates actively in the mRNA translation and DNA repair pathways. Inside the ribosome biogenesis pathway RPS3 interacts closely with other ribosomal proteins like RPS19 and RPS20 to form functional ribosomal units. In the context of DNA repair it aligns with interaction networks that overlap with the NHEJ (Non-Homologous End Joining) pathway thereby influencing genomic maintenance alongside proteins such as XRCC5.

The dysfunction of RPS3 associates with neurodegenerative diseases and certain types of cancer. Aberrant levels or mutations can disrupt protein synthesis and DNA repair mechanisms potentially leading to tumorigenesis. In the context of cancer RPS3 shows interactions with oncogenes such as MYC linking it to pathway disturbances that can facilitate tumor progression. Similarly its misregulation is noticed in neurodegenerative contexts where protein synthesis and repair imbalances contribute to disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the small ribosomal subunit (PubMed : 23636399, PubMed : 8706699). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399, PubMed : 8706699). Has endonuclease activity and plays a role in repair of damaged DNA (PubMed : 7775413). Cleaves phosphodiester bonds of DNAs containing altered bases with broad specificity and cleaves supercoiled DNA more efficiently than relaxed DNA (PubMed : 15707971). Displays high binding affinity for 7,8-dihydro-8-oxoguanine (8-oxoG), a common DNA lesion caused by reactive oxygen species (ROS) (PubMed : 14706345). Has also been shown to bind with similar affinity to intact and damaged DNA (PubMed : 18610840). Stimulates the N-glycosylase activity of the base excision protein OGG1 (PubMed : 15518571). Enhances the uracil excision activity of UNG1 (PubMed : 18973764). Also stimulates the cleavage of the phosphodiester backbone by APEX1 (PubMed : 18973764). When located in the mitochondrion, reduces cellular ROS levels and mitochondrial DNA damage (PubMed : 23911537). Has also been shown to negatively regulate DNA repair in cells exposed to hydrogen peroxide (PubMed : 17049931). Plays a role in regulating transcription as part of the NF-kappa-B p65-p50 complex where it binds to the RELA/p65 subunit, enhances binding of the complex to DNA and promotes transcription of target genes (PubMed : 18045535). Represses its own translation by binding to its cognate mRNA (PubMed : 20217897). Binds to and protects TP53/p53 from MDM2-mediated ubiquitination (PubMed : 19656744). Involved in spindle formation and chromosome movement during mitosis by regulating microtubule polymerization (PubMed : 23131551). Involved in induction of apoptosis through its role in activation of CASP8 (PubMed : 14988002). Induces neuronal apoptosis by interacting with the E2F1 transcription factor and acting synergistically with it to up-regulate pro-apoptotic proteins BCL2L11/BIM and HRK/Dp5 (PubMed : 20605787). Interacts with TRADD following exposure to UV radiation and induces apoptosis by caspase-dependent JNK activation (PubMed : 22510408).

See full target information RPS3

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

RNA (New York, N.Y.) 30:1572-1585 PubMed39293933

2024

Translation elongation inhibitors stabilize select short-lived transcripts.

Applications

Unspecified application

Species

Unspecified reactive species

Nicolle A Rosa-Mercado,Allen R Buskirk,Rachel Green

Cell chemical biology 31:1349-1362.e5 PubMed38810651

2024

Specificity profiling of deubiquitylases against endogenously generated ubiquitin-protein conjugates.

Applications

Unspecified application

Species

Unspecified reactive species

Valentina Rossio,Joao A Paulo,Xinyue Liu,Steven P Gygi,Randall W King

International journal of general medicine 16:5651-5664 PubMed38059157

2023

Lnc-SNHG5 Promoted Hepatocellular Carcinoma Progression Through the RPS3-NFκB Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Hao,Yu Liu,Fengli Guo,Guiming Fu,Junya Ning,Xianhui Ruan,Xiangqian Zheng,Ming Gao

Nucleic acids research 51:10950-10969 PubMed37811880

2023

An interaction between eIF4A3 and eIF3g drives the internal initiation of translation.

Applications

Unspecified application

Species

Unspecified reactive species

Jeeyoon Chang,Min-Kyung Shin,Joori Park,Hyun Jung Hwang,Nicolas Locker,Junhak Ahn,Doyeon Kim,Daehyun Baek,Yeonkyoung Park,Yujin Lee,Sung Ho Boo,Hyeong-In Kim,Yoon Ki Kim

Nucleic acids research 51:4208-4222 PubMed37070189

2023

Selective translational control of cellular and viral mRNAs by RPS3 mRNA binding.

Applications

Unspecified application

Species

Unspecified reactive species

Tal Havkin-Solomon,Elad Itzhaki,Nir Joffe,Nina Reuven,Yosef Shaul,Rivka Dikstein

Nucleic acids research 51:4415-4428 PubMed37013984

2023

Translation regulation of specific mRNAs by RPS26 C-terminal RNA-binding tail integrates energy metabolism and AMPK-mTOR signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Tal Havkin-Solomon,Davide Fraticelli,Anat Bahat,Daniel Hayat,Nina Reuven,Yosef Shaul,Rivka Dikstein

Viruses 14: PubMed35632744

2022

PRRSV Induces HMGB1 Phosphorylation at Threonine-51 Residue to Enhance Its Secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Wang,Jingyi Zhang,Yu Fu,Linying Jia,Yali Zhang,Liang Bai,Weirong Wang,Daxin Cheng,Enqi Liu

eLife 9: PubMed32744497

2020

EDF1 coordinates cellular responses to ribosome collisions.

Applications

Unspecified application

Species

Unspecified reactive species

Niladri K Sinha,Alban Ordureau,Katharina Best,James A Saba,Boris Zinshteyn,Elayanambi Sundaramoorthy,Amit Fulzele,Danielle M Garshott,Timo Denk,Matthias Thoms,Joao A Paulo,J Wade Harper,Eric J Bennett,Roland Beckmann,Rachel Green

Nature communications 9:2613 PubMed29973597

2018

microRNA-122 amplifies hepatitis C virus translation by shaping the structure of the internal ribosomal entry site.

Applications

Unspecified application

Species

Unspecified reactive species

Philipp Schult,Hanna Roth,Rebecca L Adams,Caroline Mas,Lionel Imbert,Christian Orlik,Alessia Ruggieri,Anna M Pyle,Volker Lohmann

Nature communications 8:15730 PubMed28589942

2017

Misfolded polypeptides are selectively recognized and transported toward aggresomes by a CED complex.

Applications

Unspecified application

Species

Unspecified reactive species

Joori Park,Yeonkyoung Park,Incheol Ryu,Mi-Hyun Choi,Hyo Jin Lee,Nara Oh,Kyutae Kim,Kyoung Mi Kim,Junho Choe,Cheolju Lee,Ja-Hyun Baik,Yoon Ki Kim

View all publications

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Product promise

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Anti-RPS3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS3 antibody (AB140688)

Immunoprecipitation - Anti-RPS3 antibody (AB140688)

Western blot - Anti-RPS3 antibody (AB140688)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Product promise